In literature, several methods have been described for analysis of Losartan potassium in API and formulations. Various methods are HPLC based [1,2], (CE) capillary electrophoresis [3], voltametric determination [4] and some are spectrophotometric [5][6][7]. But there is no single analytical method have been reported for determination of losartan as simple and economical like this method. Because I have used simple water for analysis of these all brandsand in very less time period I have analysed the drugs.we have done this types of assay for other drugs which will be useful for small scale laboratory and where expensive Author: Faculty of Pharmacy, Jinnah University for Women, Karachi, Pakistan. e-mails: safila117@yahoo.com, safila117@gmail.com instrument not available we can easily find out these drugs in a very short period of time. The serial number as an identification of purchased brands are given in Table 1. Using 20 tablets of six different brand of losartan from the marketed sample were weighed and average mean were calculated. By calculating the average weighed powder of each brand equivalent to 10 mg of losartan was transferred in a volumetric flask containing small water then solution was sonicated for about 5 min and than make up volume upto 100 ml with water.Same procedure was repeat for all brands for preparation of solutions. # e) Procedure After preparation of standard and sample solutions of different brands, strength of all solutions 100 ppm in 100 ml. By using 234 nm wavelength absorbance noted and calculate % assay of each drug. # III. # Results and Discussions Pharmaceutical assay was carried out by using spectrophotometer on six brands of losartan tablets. # Conclusion A simple, rapid, and economical UV method has been established for determination of losartan alone or in their formulations. This method has several advantages, including simple sample preparation and rapid analysis. It is suitable for analysis of antihypertensive agent losartan in their formulations in a single run, in contrast with previous published methods. This makes the method suitable for routine analysis in QC quality-control laboratories. ![Introduction osartan (fig 1) is a phenyl tetrazole substituted imidazole compound and it is a angiotensin II receptor blocker (ARB II ) type I antagonist and it is used in the treatment of hypertension. Administration of Losartan results in a decrease TPR total peripheral resistance and cardiac venous return.](image-2.png "") 1![Figure 1 : Structure of Losartan](image-3.png "Figure 1 :") ![UV visible spectrophotometer (1601), Shimadzu double beam was used to analysis of spectra. The water is used as a solvent for active and formulations.b) Wavelength SelectionAbout 100 ppm of losartan active solution was prepared in water. This solutions scanned in 200-400 nm UV region. The highest wavelength (?max) was observed at 234 nm and therefor this wavelength was used for analysis of samples.c) Standard solution of losartanAccurately weighed 10 mg of losartan was transferred to a volumetric flask and add distilled water to produce 100 ml.the conc of solution is 100 ppm in 100 ml.d) Sample Preparation of different brandsThe six different brands AZA, COZAAR, LOSAAN, ZOSTAT, LOSARK and EZIDAY purchased from different pharmacies in Karachi, Pakistan. All tablets of each brand have same batch number and were labeled to conatin losartan 50 mg . All the six brands have 5 year shelf life.](image-4.png "") - 1Absorbance atBrand Name Code Average wt of tablet mgWt for 100 ppm234 nm% assayAZALSR10.160.0162.627101.0385cozaarLSR20.1560.0152.673102.8077losaanLSR30.1530.0152.653102.0385zostatLSR40.180.0182.647101.8077losarkLSR50.2340.0232.639101.5ezidayLSR60.1750.0172.675102.8846 © 2014 Global Journals Inc. (US) * Simultaneous determination of losartan and hydrochlo-rothiazide in tablets by high-performance liquid chromatography GCarlucci GPalumbo PMazzeo MGQuaglia J Pharm Biomed Anal 23 2000 * Determination of Losartan, Telmisartan, and Valsartan by Direct Injection of Human Urine into a Column-Switching Liquid Chromatographic System with Fluorescence Detection MariaDel Rosario BContreras YClavijo STorres DDelgado Y J Pharm Biomed Anal 50 2009 * Comparison of liquid chromat-ography, capillary electrophoresis and super-critical fluid chromatography in the determination of Losartan Potassium drug substance in Cozaar tablets RCWilliams MSAlasandro VLFasone RJBoucher JFEdwards J Pharm Biomed Anal 14 1996 * HIHabib SAWeshahy SToubar MmaEl-Alamin 2008 * Development and validation of an UV derivative spectrophotometric determination of Losartan potas-sium in tablets OCLastra IGLemus HJSã¡nchez RFParez J Pharm Biomed Anal 33 2003 * Determination of losartan and triamterene in pharmaceutical compounds and urine using cathodic adsorptive stripping voltammetry AAEnsafi RHajian Anal Sci 24 2008