# Introduction odern world requires people to adapt to the increased stress on the psyche associated with economic and political instability, social problems, and man-made and environmental factors, which together lead to the development of urban stress, accompanied by fatigue, irritability, tension and even unmotivated hatred and aggression [1]. When the body is under the influence of extreme environmental factors, both physiological shifts and psychological changes of varying degrees of severity can occur, with a pattern of manifestations commonly including a kind of "blockade" of cognitive processes, in which the volume of perception narrows, synthesis processes in thinking are disrupted, and purposeful behaviour becomes disorganized [2]. Accordingly, the discovery, development and use of drugs that increase stress resistance, resistance Research on the need for new drugs for those neurological disorders in Uzbekistan has been done and successfully produced an injectable solution of Seroxidol. This drug is an inhibitor of free radical processes and a membrane protector with antihypoxic, stress-protective, nootropic, anticonvulsant and anxiolytic effects. Seroxidol increases the resistance to the effects of various damaging factors (shock, hypoxia and ischaemia, cerebral circulation disorders, alcohol intoxication and antipsychotic drugs with neuroleptics) and improves the functional state of the ischaemic myocardium. It effectively restores myocardial contractility in cases of reversible cardiac dysfunction. Seroxidol contains ethyl methyl hydroxypyridine succinate and sodium metabisulfite. # Purpose of the study: This study pursued the development of a method for the quantitative determination of ethyl methyl hydroxypyridine succinate by UV spectrophotometry of the drug Seroxidol in a 50 mg/ml solution for injection and its bioequivalence. # II. # Materials and Methods Class A volumetric glassware was used in the work: conical flasks of 50 ml and 100 ml, volumetric pipettes, an AS-220/X ser # B635963283 analytical balance (Ohaus, Germany), a SHIMADZU UV-1900UV spectrophotometer, and cuvettes with a thickness of 10 mm. The object of the study was injection solutions corresponding to the TPA Seroxidol, a 50 mg/ml solution for injection. Determination was carried out by UV spectrophotometry. The acute toxicity of the preparations was studied in 60 white mice of both sexes, weighing 19-21 g. From the compared preparations Seroxidol produced by LLC "MEDIOFARM", Uzbekistan and "Mexidol®" produced by LLC "Ellara", Russia, a 2.5% solution was prepared and administered to the mice once intravenously at doses of 150 mg/kg, 175 mg/kg, 200 mg/kg, 225 mg/kg and 250 mg/kg (0.12-0.2 ml) [3]. The animals were kept under continuous observation during the first hour, then under hourly observation throughout the first day of the experiment and once a day for the next 13 days of the experiment. As indicators of the functional state of animals, the general condition of the mice and their behaviour, the intensity and nature of the motor activity, the presence of seizures, coordination of movements, reaction to external stimuli and tone of skeletal muscles, appetite, body weight, and number and consistency of faecal masses were monitored [7]. During the experiment, the clinical state of the animals was monitored: the presence/absence of signs of poisoning, the time of their appearance, and death. All experimental animals were kept under standard conditions on a common diet with free access to water and food [3]. After completion of the experiment, the average lethal doses (LD50) were determined [5]. # III. # Results and Discussion Two millilitres of the medicine were placed in a volumetric flask with a capacity of 100 ml, and the volume of the solution was brought up to the mark with 0.01 mol/l hydrochloric acid and stirred. Next, 1.0 ml of the resulting solution was placed in a 100 ml volumetric flask, and the volume of the solution was brought to the mark with 0.01 mol/L hydrochloric acid and mixed (test solution). The optical density of the resulting solution was measured on a spectrophotometer at the absorption maximum at a wavelength of 297 nm in a cuvette with a layer thickness of 10 mm, using 0.01 mol/L hydrochloric acid as a reference solution. (accurately weighed) of ethyl methyl hydroxypyridine succinate was placed in a volumetric flask with a capacity of 100 ml, and 50.0 ml of 0.01 mol/l hydrochloric acid solution was added. ?? = ?? 1 ??? 0 ? 1 ? 100 ? 100 ? ?? ? (100 ? ??) ?? 0 ? 2 ? 1 ? 100 ? 1 ? 100 ? 100 ? 100 = ?? 1 ??? 0 ? ?? ? (100 ? ??) ?? 0 ? 20000 , After the complete dissolution of the sample, the volume of the solution was brought to the mark with the same solvent and mixed. Then, 1.0 ml of the resulting solution was placed in a 100 ml volumetric flask, the volume of the solution was brought to the mark with the same solvent, and the solution was mixed. One millilitre of WS contained approximately 0.00001 g of ethyl methyl hydroxypyridine succinate. The solution must be freshly prepared. The data obtained are presented in Figures 1, 2 and 3. Experiments have shown that after a single intravenous administration of the Seroxidol preparation, produced by MEDIOFARM LLC, Uzbekistan, and Mexidol®, produced by Ellara LLC, Russia, at a dose of 150 mg/kg, no visible changes were observed in the behaviour and functional state of the animals. All mice were active and responded to external stimuli, and food and water consumption was normal. No pathological changes in the hair and skin, diuresis, no diuresis, and no changes in the consistency and amount of faeces were observed. No signs of intoxication were observed. In this group, until the end of the experiment, no deaths were observed among the animals. When the drugs were administered at a dose of 175 mg/kg, the mice developed clonic-tonic seizures, 1 mouse died in the generic group, and 2 mice died in the comparison group. [4] When the drugs were administered at a dose of 200 mg/kg, a decrease in motor activity, rapid breathing, impaired coordination of movements, a weakening of the reaction to external stimuli, and a decrease in food and water consumption were observed in experimental animals. Three mice died in the comparison group. At a dose of 225 mg/kg, the animals ceased to respond to external stimuli, and no food or water consumption was observed. During the experiment, 5 individuals in each group died [4]. The administration of a dose of 250 mg/kg of either Seroxidol produced by LLC "MEDIOFARM", Uzbekistan, or "Mexidol®" produced by LLC "Ellara", Russia, caused the immediate death of some animals after the administration of the medicine. By the end of the experiment, the condition of the surviving animals returned to normal as the signs of intoxication decreased. The LD50 of the drug Seroxidol produced by LLC "MEDIOFARM", Uzbekistan, was 200.0 (188.7 ÷ 211.1) mg/kg. The LD50 of the drug "Mexidol®" produced by LLC "Ellara", Russia, was 193.9 (178.5 ÷ 209.6) mg/kg. The acute toxicities of the compared drugs are shown in Table 2. IV. # Conclusion This method was carried out in accordance with the requirements of the TPA. One millilitre of the drug Seroxidol contained 0.049 mg of ethyl methyl hydroxypyridine succinate. Thus, the data obtained show that the preparations Seroxidol (50 mg/ml solution for injection) produced by "MEDIOFARM" LLC (Uzbekistan) and "Mexidol®" (50 mg/ml solution for injection)produced by "Ellara" LLC, (Russia) at 5 ml each are biologically equivalent in terms of acute toxicity. 12![Fig. 1: Typical spectrum of a standard sample of the tested succinate](image-2.png "Fig. 1 :Fig. 2 :") 3![Fig. 3: Spectrum of the "placebo" solution](image-3.png "Fig. 3 :") 1? ,%fS 2SÎ?" ?? , %X 1 =0.0490.04940.00000230.001520.00423.812X 2 =0.047X 3 =0.051X 4 =0.050? 5 =0.050 2Seroxidol produced by LLC "MEDIOFARM", Uzbekistan"Mexidol®" produced by LLC "Ellara", Russia? animalsweight, gdose mg/k g mlmethod of administration lethalityweight, gdose mg /kg mlway introduction lethality1210.13No210.13No2200.12No200.12No3211500.13i/vNo191500.11i/vNo4200.12No210.13No5200.12No210.13No6210.13No190.11No1200.14No200.14No2190.13death200.14death3 419 191750.13 0.13i/vNo No21 191750.15 0.13i/vNo death5200.14No200.14No6210.15No190.13No1210.17death210.17death2200.16death200.16No3 419 192000.15 0.15i/vNo death21 192000.17 0.15i/vdeath No5200.16No210.17No6200.16No210.17death1190.17death200.18death2190.17No210.19death3 420 202250.18 0.18i/vdeath death20 212250.18 0.19i/vdeath No5210.19death190.17death6210.19death210.19death1200.20death210.21death219210.19death21200.21death3 420 20192500.21 0.20i/?vdeath death19 21212500.20 0.19i/vdeath death50.20death0.21death60.19death0.21deathLD 50200.0 (188.7÷211.1) mg/kg193.9 (178.5÷209.6) mg/kg * Stress factors in the socio-cultural space of a modern large kind // Izvestia Volg GTU ABarkovskaya Yu MPNazarova 2014. -T. 16. -No. 5. -S. 37-42 Barkovskaya A.Yu., Nazarova M.P 2014 16 Stress factors in the socio-cultural space of the modern large genus. News of VolgSTU. InRuss). * Extreme conditions of action: concept, content, classification // Bulletin of the KRSU GSZaitsev No. 10. -S. 25-29 2014 4 Zaitsev G.S. Extreme conditions of professional activity: concept, content and classification. InRuss). * Mavlanov Sh.R. Study of the bioequivalence of the drug "Seroxidol". Infection, immunity and pharmacology DMSarvarova NAYunuskhodjaeva Journal 2181-5534. ?6/2021 * Methods of physical and chemical analysis of drugs QAUbaydullayev AAMukhitdinov Tashkent-2017 Part II. 3-chapter 58 page. 240 p * Methods of spectrophotometry in the UV and visible regions in inorganic analysis ZMarchenko MBalcezhin Binom Knowledge Laboratory-2014 * A review of the epidemiological evidence for the 'antioxidant hypothesis'. // Public health nutrition SAStanner JHughes CNKelly JButtriss 10.1079/PHN2003543 15153272 7