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\title{Study on Prevalence and Monetary Loss Attributed to Hydatidosis in Cattle Slaughtered at Jimma Municipal Abattoir, Southwestern Ethiopia}
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             \author[1]{Mukarim  Abdurahaman}

             \author[2]{Ayub  Temam}

             \author[3]{Benti  Deresa}

             \affil[1]{  Jimma University}

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\date{\small \em Received: 8 December 2015 Accepted: 4 January 2016 Published: 15 January 2016}

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\begin{abstract}
        


A cross-sectional study was conducted on bovine hydatidosis from November 2015 to June 2016 with the objectives of investigating its prevalence and Monetary loss in cattle slaughtered in Jimma municipality abattoir. Routine ante mortem and post-mortem inspection was performed on a total of 400 selected slaughtered cattle. Infection organs 223 cattle positive, 200 (89.7%) had cysts more in lungs, 20 (8.9%) in liver, 1 (0.45%) in kidney, 1(0.45%) in spleen, whereas, the rest of 1(0.45%) in heart infections involved organs. A significant association was observed (P<0.05) between the disease positivity and age groups, body condition. It was concluded that these zonotic cestodes deserve due attention to safeguard public health and that further studies are needed on epidemiology and public health importance of Echinococcus granulosus in the study area.

\end{abstract}


\keywords{bovine, hydatidosis, prevalence, monetary loss jimma, abattoir.}

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\let\tabcellsep& 	 	 		 
\section[{I. Introduction}]{I. Introduction}\par
ydatidosis caused by the larval stage (metacestode) of Echinococcus granulosus is the most widespread parasitic zoonoses \hyperref[b27]{(Ibrahim, 2010;}\hyperref[b24]{Getaw et al., 2010)}. Dogs are the usual definitive hosts while a large number of mammalian species are intermediate hosts, including domestic ungulates and man. It is a familiar with many different countries (cosmopolitan) zoonotic infection \hyperref[b6]{(Azlaf and Dakkak, 2006)}.\par
Despite the large efforts that have been put into the research and control of echinococcosis, it still remains a disease of worldwide significance. In some areas of the world, Cystic echinococcosis caused by E. granulosus is a re-emerging disease in places where it was previously at low levels (Urquhart et al., 1996;  Kebede et al., 2009a).\par
Echinococcus granulosus infection is endemic in East and South Africa, Central and South America, South Eastern and Central Europe, Middle East, Russia and China. The highest incidence is reported mainly from sheep and cattle rearing areas \hyperref[b4]{(Arene, 1995)}. The disease is most important in livestock production which is based mainly on extensive grazing system. Several reports from different parts of Ethiopia indicate that Author ? ? ?: Jimma University, College of Agriculture and Veterinary Medicine (JUCAVM), P.O.Box 307. e-mails: ayubtemam1@gmail.com, batijidu@yahoo.com, mukevet@yahoo.com hydatid cyst is prevalent in livestock population of the country \hyperref[b28]{(Jobre et al., 1996;}\hyperref[b31]{Kebede et al., 2010)}.\par
According to Abebe and Yilma (2011) a prevalence of 72.4\%, 37.72\%, 33.78\% and 13.7\% in cattle slaughtered in Asella, Adama, Gonder, and Dire Dawa was documented respectively indicating its importance in the livestock industry. Its distribution is higher in developing countries especially in rural communities where there is close contact between dogs (definitive host) and various domestic animals intermediate hosts \hyperref[b17]{(Eckert and Deplazes, 2004)}. By affecting many animal species, intermediate animal hosts and humans, hydatid cyst causes tremendous economic losses worldwide and specially in those areas where the parasite is endemic  {\ref (Urquhart et al., 1996)}.\par
Knowledge about the prevalence of the diseases together with associated risk factors as part of the epidemiology of the disease is crucial for any attempt of prevention and control of the disease in question. Moreover, determination of the economic significance of the disease is important for decision making, planning, and implementation of local control strategies. The present study were, therefore, conducted in the area with objective of determining the prevalence of Hydatidosis, its associated risk factor in cattle slaughtered at Jimma municipal abattoir and to estimate the economic significance of the disease in cattle. 
\section[{II. Material and Method a) Study area}]{II. Material and Method a) Study area}\par
The study was conducted in Jimma town which is located at about 352km south west of Addis Ababa. The area receives a bimodal rain fall with an average annual rain fall of 1530mm. The long rainy season occur during the months of June to September while the short rainy season occurs during the months of March to May. The climatic condition of the town is "Weynadega" and the town is located at an altitude of 1915masl. The annual maximum and minimum temperature ranges from 24-30C? and 7-14C? respectively. According to the statistical data obtained (CSA, 2009), Jimma district has a livestock population of 2,016,823 cattle, 288,411 goats, 942,908 sheep and 74574 horses, 49,489 donkey, 28,371 mules and 1,139,735 poultry. 
\section[{b) Study animals}]{b) Study animals}\par
The study animals were local breeds of cattle coming from different wereda of the Jimma zone to Jimma municipal abattoir. Only male cattle and sheep were slaughtered, but majority of animals slaughtered in the abattoir were male cattle. The majority of slaughter animals came from seven weredas, this are Agaro, Asandabo, Bilida, Dedo, Jimma, Sarbo and seka. The body condition score was classified into poor, medium and good (fat),  {\ref (Nicholson and Butter worth, 1986)}. The age was determined by dentition formula according to the method described by De Lahunta and Habel (1986), and animals categorized into three age groups (< or = 5, 5 -8, and > 8 years). 
\section[{c) Sample size and sampling method}]{c) Sample size and sampling method}\par
The study animals were selected from the slaughter line using simple random sampling technique. The required sample size was determined based on prevalence of 61\% \hyperref[b34]{(Koskei, 1998)} using the formula given by \hyperref[b48]{(Thrusfield, 2005)}. The study considered 95\% confidence interval and 5\% precision level. Accordingly a total of 384 animals were calculated, but to increase precision, the number of examine animals were reached to 400. For this study sex, age, origin and body condition of animals were considered as risk factors. 
\section[{d) Study Design}]{d) Study Design}\par
A cross sectional study was conducted from November 2015 to June, 2016 by collecting data on events associated with hydatidosis in cattle slaughtered in Jimma municipal abattoir. This study was conducted to determine update information on the prevalence and economic impact on bovine hydatidosis at Jimma municipal abattoir. (Two slaughtering days per week) visits were made to abattoir. 
\section[{e) Study Methodology i. Ante mortem inspections}]{e) Study Methodology i. Ante mortem inspections}\par
Pre-slaughter examinations of cattle were conducted in the lairage in order to determine the sex, age, body condition and origin of animals. Identification number was given for each animal to examine after evisceration. During ante-mortem examination, animals were clinically examined for any sign of illness while standing and moving according to  {\ref (Urquhart et al., 1996)}. And following the judgments passed by \hyperref[b19]{(FAO, 1994)}. 
\section[{ii. Post mortem inspection}]{ii. Post mortem inspection}\par
During post mortem examination organs especially liver, lung, spleen, kidney and heart as a whole were systematically inspected for the presence of hyatid cyst by applying the routine meat inspection procedure of primary examination followed by secondary examination. The primary examination involves visualizations and palpation of organs, were as secondary examination involves further systemic incision of each organs into pieces and whenever evidence of hydatid cyst was found, it was classified as live or calcified and the cyst distribution into organs was recorded. 
\section[{iii. Examination of cysts for fertility and viability}]{iii. Examination of cysts for fertility and viability}\par
Based on the presence or absence of brood capsules containing protoscolices in hydatid fluid, cysts were identified and classified as fertile and infertile according to the method described by \hyperref[b35]{Macpherson (1985)}. Individual hydatid cysts were carefully incised and examined for protoscolices, which resembled white dots on the germinal epithelium; such cysts were characterized as fertile cysts.\par
Fertile cysts were subjected to viability test. A drop of the sediment containing the protoscolices were placed on the microscope glass slide and covered with cover slip and observed for amoeboid like peristaltic movements with 40x objective. For clear vision, a drop of 0.1\% aqueous eosin solution was added to equal volume of protoscolices in hydatid fluid on microscope slide with the principle that viable protoscolices should completely or partially exude the dye while the dead ones absorb it \hyperref[b35]{(Macpherson et al., 1985)}. Furthermore, infertile cysts were further classified as sterile or calcified. Sterile hydatid cysts were characterized by their smooth inner lining usually with slightly turbid fluid in their content. Typical calcified cysts produce a grittysound heard at incision \hyperref[b46]{(Soulsby, 1982}).\par
An attempt was made to estimate the annual economic losses from hydatidosis in cattle taking into account losses from cost of organ condemnation and from carcass weight.The retail market price of average size offal (lung, liver, kidney, heart and spleen) and the cost of one kg beef were obtained from information gathered from local butchers. Annual economic loss due to organ condemnation was determined by considering annual slaughter rate of cattle and prevalence of hydatidosis per organ and an estimated 5\% carcass weight loss  {\ref (Getaw et}   
\section[{iv. Determination of Monetary losses due to hydatid cyst}]{iv. Determination of Monetary losses due to hydatid cyst}\par
Phr-percent involvement of heart Cphr-current mean retail price of heart Pli-percent involvement of liver Cpli -current mean retail price of liver Psp-percent involvement of spleen Cpsp -current mean retail price of spleen. Pkid-percent involvement of kidney Cpkid -current mean retail price of kidney N: B-All prices are determined from the price at Jimma town.\par
Total economic loss was evaluated by considering both loss from organ condemnation and loss from carcass weight loss. Total loss = direct loss (loss from organ condemnation) + indirect loss (loss from carcass weight loss). 
\section[{f) Data Analysis and Management}]{f) Data Analysis and Management}\par
The data obtained was coded in Microsoft excel sheet 2007 and subjected to descriptive statistics and chi-square in order to assess the magnitude of the difference of comparable variables using SPSS version 20.0 software. Statistically significant association between variables is considered to exist if the p-value is less than 0.05. 
\section[{III. Results}]{III. Results} 
\section[{a) Prevalence and Risk Factors i. Age group}]{a) Prevalence and Risk Factors i. Age group}\par
Out of the total 400 heads of cattle slaughtered and examined, 218 (54.5\%) were infected with hydatid cyst, more cysts involving different visceral organs (lung and liver). Rate of infection in different age groups (<5 and, 5-8 and >8 years) was assessed and described in (Table \hyperref[tab_0]{1}). Prevalence in age groups have shown as statistically highly significant variation (P<0.05, ?2 = 16.615) with young group having higher infections.\par
ii. Body condition score Prevalence was also assessed in terms of body condition score (Table \hyperref[tab_1]{2}). It was found that cattle having poor body condition had the highest prevalence (74\%) followed by medium (46.6\%) and good (52.5\%). There was highly significant difference revealed between body condition scores (P<0.05, ?2=28.332) with poor animals groups having higher infections.\par
iii. Origin of animals Prevalence of Hydatidosis in cattle slaughtered at Jimma Municipal abattoir in origin of animals at Bilida (61\%) was higher infected but, at Sarbo 43.5\% was less infected (Table \hyperref[tab_2]{3}). 
\section[{b) Cyst Distribution}]{b) Cyst Distribution}\par
Overall distribution of cysts in different organs of cattle slaughtered at Jimma Municipal abattoir was described (Table \hyperref[tab_3]{4}). Of the 223 cattle positive, 200 (89.7\%) had cysts merely in lungs, 20 (8.9\%) in liver, 1 (0.45\%) in kidney, 1(0.45\%) in spleen, whereas, the rest of 1(0.45\%) in heart infections involved organs. 
\section[{c) Characters of hydatid cyst in different organs}]{c) Characters of hydatid cyst in different organs}\par
Out of 98 organ infected by cysts to tested for fertility, 50(17.3\%) cysts of lung, 45(54.87\%) cyst of liver, 1(100\%) cysts of kidney,1(100\%) cysts of spleen, and 1(100\%)cysts of heart origins had protoscolices detected and hence, fertile. Out of the total cyst counts, 98(26.2\%) cyst counts are fertile, 216(57.8\%) are sterile and 60(16\%) calcified. Fertility status of cysts from different organs has shown, but the cysts of lung origin being highly fertile (Table \hyperref[tab_4]{5}).\par
Due to aesthetic value and to break the life cycle of the Echinococcus parasites infected organs are condemned. A total of lung, liver, kidney, spleen and heart were condemned due to hydatidosis with an economic loss of 89249.2ETB, 22312.3 ETB, 676.89 ETB, 225.63ETB and 676.89 ETB respectively. The direct and indirect economic loss was about 133140.91 ETB and 3249072 ETB respectively. The total annual financial loss due to bovine hydatidosis was estimated to be 3362212.9 ETB, (Table \hyperref[tab_5]{6}).    
\section[{IV. Discussion}]{IV. Discussion}\par
In the present study the prevalence of Bovine hydatidosis in Jimma Municipal abattoir was found to be 54.5\% which was comparable with the results of other works conducted, this study was much higher compared to the prevalence reported at Jimma 31.44\% \hyperref[b49]{(Tolossa et al,. 2009}) and 22.4\% \hyperref[b38]{(Moges, 2003)}, Konso 22.57\% \hyperref[b22]{(Fikre, 1994)}, Adigrat 20.3\% \hyperref[b33]{(Kebede, et al., 2009b}) and Nekemte 31.19\% \hyperref[b21]{(Feyissa, 1987)}. Much lower prevalence was also reported by Kebede, 2009b (7.5\%) in Shire and Tsehaye, 1995 (7.2\%) in Debre Birhan and also high in Asella 61.0\% \hyperref[b34]{(Koskei, 1998)}, 62.96\% around Bale Robe \hyperref[b55]{(Woubet, 1988)}, and 59.9\% Bahirdar \hyperref[b39]{(Nebiyou, 1990)}.\par
The present prevalence rate was high (54.5\%). This might be due to the abundance and frequent contact between the intermediate and infected final hosts. It could also be associated to slaughtering of aged cattle which have had considerable chance of exposure to the parasitic ova, backyard slaughtering of small ruminants and provision of infected offal's to pet animals around homesteads. Moreover, poor public awareness about the disease and presence of few slaughter houses could have contributed to such a higher prevalence rate.\par
Generally, variation among the prevalence of hydatidosis at different geographical location could be associated to the strain difference of Echinococcus granulosus that exist in different geographical locations \hyperref[b36]{(McManus, 2006)}. Additionally variation could be with age factors of the animals and other factors like difference in culture, socio-economical activities and attitudes to dogs and their population. Similar to the present finding, it was reported that cystic Echinococcosis infection was higher for older animals (Azlaff \& Dakkak, 2006; Fayesa et al., 2010). Animals with more than eight years of age were found to be highly infected that stastically significant (P value < 0.05). This could be mainly due to the fact that aged animals have longer exposure time to Echinococcus granulosus eggs. In addition, older animals might have weaker immunity to combat against infection \hyperref[b26]{(Himonas, 1987)}. This finding is similar to the finding of Fikre Lobago (1994), Hagos Yihdego (1997), \hyperref[b52]{Umur (2003)}, \hyperref[b6]{Azlaf and Dakkak (2006)} and \hyperref[b18]{(Esatgil and Tuzer, 2007)}.\par
The prevalence of hydatidosis by origin of slaughtered cattle was assessed and statistically significant difference (P value < 0.05) was found indicating that geographical regions play an important role in distribution of the cysts. This could be due to the difference in the socio-economic status and animal husbandry practices of community in all areas from where animals were brought for slaughter and frequent contact of animals with infected definite host.\par
The prevalence of hydatidosis among different organs involved in harboring of the cyst showed that lung was found to be the most commonly affected organ (50\%) followed by liver (43\%) and this was equivalent with Bizuwork (2013), 50.5\% for lung and 40.6\% for liver and also similar result of 54.5\% and 43.5\% was reported by Debas and Ibrahim (2013) on lung and liver respectively. This finding was higher than finding of Abunna et al., (2012) who reports 12.5\% and 4.25\% prevalence for lung and liver respectively while 92.7\% in lung and 53.2\% in liver which is higher than this study was also reported by Abera et al.,  {\ref (2013)}.\par
In this study number of cysts collected from lung 200(89.7\%) was greater than that collected from liver 61(8.9\%) and that of spleen, heart and kidney in which 1(0.45\%) was recorded. Comparable results were reported by (Alemu and Yitagele, 2013), 47.04\% and 44.2\% for lung and liver respectively and 9.41\% for spleen, heart and kidney. This might be due to the fact that cattle are slaughtered at older age, during the time the liver capillaries are dilated and most oncospheres directly pass to the lung; additionally, it is possible for the hexacanth embryo to enter the lymphatic circulation In direct Monetary loss Total Monetary loss=direct loss + in direct loss and be carried via the thoracic duct to the heart and lungs in such a way that the lungs may be infected before or instead of liver \hyperref[b5]{(Arene, 1985)}.\par
Additionally, the lung and liver which are most commonly infected organs, this could be due to the fact that lungs and livers posses the first great capillaries of sites encountered by migrating Echinocooccus onchosphere (hexacanth embryo) which adopt the portal vein route the first large capillaries encountered by migrating blood borne onchospheres and primarily negotiate pulmonary and hepatic filtering system sequentially before any other organ is involved. However, development of hydatid cysts occur occasionally in other organs like spleen, kidney and heart and other organs and tissues when onchosphers escaped into general systemic circulation  {\ref (Urquhart et al., 1996)}.\par
Lung harbored highest number of calcified cysts (20.4\%) followed by liver (1.2\%). This finding is comparable with finding of \hyperref[b37]{Mesele et al. (2013)} 60.2\% ,22.6\% and 4.3\% respectively for lung, liver and spleen and higher than the report of \hyperref[b10]{Bizuwork et al.(2013)} with the prevalence's of 36.8\% for lung,14.6\% for liver and 0\% for spleen. This can be due to the host defence mechanisms of killing more efficiently the parasitic larvae at the early stage of development \hyperref[b26]{(Himonas, 1987)}.\par
The percentage of fertile cysts in this study was 26.2\%. This is higher compared to the fertility rate of 26.9\%, 24.4\% and 19.3\% reported by Fayesa et al.\par
(2010), \hyperref[b45]{Solomon (2011)} and \hyperref[b56]{Zelalem (2008)} respectively from different parts of Ethiopia. But the present study was quite lower compared to the 96.9\% reported from South Africa \hyperref[b5]{(Arene, 1985)}. Yet much lower fertility record such as 1.76\%, 9.85\% and 6.2\% were reported in cattle from Wolayita Soddo \hyperref[b42]{(Nigatu, et al., 2009)}, Nekemt \hyperref[b9]{(Bersissa, 1994)} and Bahir Dar  {\ref (Nebiyou,1990)} respectively. The variation in fertility rates among different species and in different geographical Zones could be due to difference in strain of Echinococcus granulosus (McManus, 2006). Strain of the parasite and the host can modify the infective pattern of the parasite \hyperref[b23]{(Gemmel et al., 2002)}.\par
Comparison of fertile cyst from different organs was found to be lower for lung (17.3\%) than liver (54.87\%). This finding was agreement with finding of (Debele et al., 2014) 66.7 and 40.7\% for lung and liver respectively and the present finding was higher than Debas and Ibrahim(2013) 25\% and 7.5\% for lung and liver respectively.\par
Out of a total 400 cattle carcasses, 218(54.5\%) were infected with hydatid cysts. Of these cysts 98 (20\%) fertile, 216(57.75\%) sterile and 60(16\%) were calci fied. Higher infected when compare with \hyperref[b49]{(Tolossa et al,. 2009}) in Jimma out of a total 512 cattle, 161(31.44\%) were infected with hydatid cysts. a total of 1171 hydatid cysts being collected from the infected animals. Of these cysts, 223(19.4\%) were fertile, 505(43.13\%) were sterile and 349(29.80\%) calcified. These indicate that cattle are an important intermediate host for the perpetuation of the life cycle of the parasite in Jimma and its surroundings.\par
The annual economic loss incurred by hydatidosis was calculated to be 3362212.9 ETB. The result was relatively comparable with the report of \hyperref[b56]{(Zelalem, 2008)}  As described above Hydatid disease is generally considered to be a rural disease because of its way of transmission cycle, which involves domestic herbivorous animals (cattle, sheep, pigs and so on) and dogs. However, it is possible that urban residents may have been in contact with Echinococcus granulosus eggs, in this matter backyard slaughtering and inappropriate disposal affected organs plays major role for the continuity of parasite life cycle. 
\section[{V. Conculusion}]{V. Conculusion}\par
The overall prevalence observed in the study indicated relatively high and an important zoonotic disease in the area and this could be due to several factors of which keeping dogs in close association with cattle. Hydatidosis also causes substantial visible and invisible economic losses in cattle of the study area as a result of condemnation of edible offal and carcass weight loss. The most preferred predilection sites of hydatid cyst in cattle like liver, kidney, heart and lungs and condemnations of these important organs having a single or multiple hydatid foci is really a huge loss. From the result obtained in the present study and considering the reality in Jimma municipal abattoir and its surrounding, it is mandatory for launching a control program proper disposal of affected offal's freely for dogs and wild canids (the usual practice in the community) should be stopped and all the condemned organs should be either buried or incinerated. Moreover, further studies are needed on genotyping, epidemiology and public health importance of Echinococcus granulosus in the study area 
\section[{VI. Acknowledgments}]{VI. Acknowledgments}\par
The authors acknowledge Jimma University College of Agriculture and Veterinary Medicine (JUCAVM) for financing the project in the period from November 2016 to June 2016. \begin{figure}[htbp]
\noindent\textbf{}\includegraphics[]{image-2.png}
\caption{\label{fig_0}}\end{figure}
  \begin{figure}[htbp]
\noindent\textbf{1} \par 
\begin{longtable}{P{0.3122037914691943\textwidth}P{0.17523696682464454\textwidth}P{0.11078199052132702\textwidth}P{0.08459715639810426\textwidth}P{0.16718009478672985\textwidth}}
v. Total Monetary Loss Estimation\tabcellsep \tabcellsep \tabcellsep \\
\tabcellsep \tabcellsep \tabcellsep \tabcellsep Year 2016\\
\tabcellsep \tabcellsep \multicolumn{2}{l}{d) Estimated Monetary loss incurred by hydatidosis}\tabcellsep Volume XVI Issue II Version I\\
\tabcellsep \tabcellsep \tabcellsep \tabcellsep D D D D )\\
\tabcellsep \tabcellsep \tabcellsep \tabcellsep ( G\\
\tabcellsep \tabcellsep \tabcellsep \tabcellsep Medical Research\\
Age group (years) Group 1 (< 5years (young)), Group 2 (5-8 years (adult))\tabcellsep \multicolumn{3}{l}{Number of cattle examined Infected Infected Prevalence 38 23 62.2\% 303 159 52.3\%}\tabcellsep Global Journal of\\
Group3 (>8years (old))\tabcellsep 39\tabcellsep 36\tabcellsep 61\%\\
Total\tabcellsep 400\tabcellsep 218\tabcellsep 54.5\%\\
?2 = 16.615, P = 0.034\tabcellsep \tabcellsep \tabcellsep \\
\tabcellsep \tabcellsep \tabcellsep © 2016 Global Journals Inc. (US)\end{longtable} \par
 
\caption{\label{tab_0}Table 1 :}\end{figure}
 \begin{figure}[htbp]
\noindent\textbf{2} \par 
\begin{longtable}{P{0.36258741258741256\textwidth}P{0.15454545454545454\textwidth}P{0.10104895104895105\textwidth}P{0.23181818181818178\textwidth}}
Body condition score\tabcellsep Animals\tabcellsep \tabcellsep Animals\\
\tabcellsep Examined\tabcellsep Infected\tabcellsep Prevalence\\
Poor\tabcellsep 77\tabcellsep 57\tabcellsep 74\%\\
Medium\tabcellsep 146\tabcellsep 68\tabcellsep 46.6\%\\
Good\tabcellsep 177\tabcellsep 93\tabcellsep 52.5\%\\
Total\tabcellsep 400\tabcellsep 218\tabcellsep 54.5\%\\
?2 = 28.332 , P = 0.00\tabcellsep \tabcellsep \tabcellsep \end{longtable} \par
 
\caption{\label{tab_1}Table 2 :}\end{figure}
 \begin{figure}[htbp]
\noindent\textbf{3} \par 
\begin{longtable}{P{0.33190476190476187\textwidth}P{0.14166666666666666\textwidth}P{0.14166666666666666\textwidth}P{0.23476190476190478\textwidth}}
Origin of animals\tabcellsep Number of examined\tabcellsep Number of infected\tabcellsep Total \% of infected\\
Agaro\tabcellsep 48\tabcellsep 28\tabcellsep 58\%\\
Asandabo\tabcellsep 45\tabcellsep 26\tabcellsep 57\%\\
Bilida\tabcellsep 94\tabcellsep 58\tabcellsep 61\%\\
Dedo\tabcellsep 59\tabcellsep 30\tabcellsep 50\%\\
Jimma\tabcellsep 48\tabcellsep 25\tabcellsep 52\%\\
Sarbo\tabcellsep 79\tabcellsep 35\tabcellsep 43.5\%\\
Seka\tabcellsep 28\tabcellsep 16\tabcellsep 56.5\%\\
Total\tabcellsep 400\tabcellsep 218\tabcellsep 54.5\%\\
X 2 = 64.742, p = 0.000\tabcellsep \tabcellsep \tabcellsep \end{longtable} \par
 
\caption{\label{tab_2}Table 3 :}\end{figure}
 \begin{figure}[htbp]
\noindent\textbf{4} \par 
\begin{longtable}{P{0.39580152671755725\textwidth}P{0.16870229007633586\textwidth}P{0.28549618320610687\textwidth}}
Organs affected\tabcellsep \tabcellsep \\
\tabcellsep Number of cases\tabcellsep Percentage\\
Lung only\tabcellsep 200\tabcellsep 89.7\%\\
Liver only\tabcellsep 20\tabcellsep 8.9\%\\
Kidney only\tabcellsep 1\tabcellsep 0.45\%\\
Spleen\tabcellsep 1\tabcellsep 0.45\%\\
Heart\tabcellsep 1\tabcellsep 0.45\%\\
Total\tabcellsep 223\tabcellsep 100\%\end{longtable} \par
 
\caption{\label{tab_3}Table 4 :}\end{figure}
 \begin{figure}[htbp]
\noindent\textbf{5} \par 
\begin{longtable}{P{0.85\textwidth}}
abattoir\end{longtable} \par
 
\caption{\label{tab_4}Table 5 :}\end{figure}
 \begin{figure}[htbp]
\noindent\textbf{6} \par 
\begin{longtable}{P{0.25032362459546925\textwidth}P{0.2365695792880259\textwidth}P{0.1733009708737864\textwidth}P{0.055016181229773455\textwidth}P{0.03576051779935275\textwidth}P{0.09902912621359224\textwidth}}
Year 2016\tabcellsep \tabcellsep \tabcellsep \tabcellsep \\
20\tabcellsep \tabcellsep \tabcellsep \tabcellsep \\
Volume XVI Issue II Version I\tabcellsep \tabcellsep \tabcellsep \tabcellsep \\
D D D D )\tabcellsep \tabcellsep \tabcellsep \tabcellsep \\
( G\tabcellsep \tabcellsep \tabcellsep \tabcellsep \\
Medical Research\tabcellsep \tabcellsep \tabcellsep \tabcellsep \\
Global Journal of\tabcellsep \tabcellsep \multicolumn{3}{l}{condemned and their price in ETB during study period}\\
Organs\tabcellsep \multicolumn{4}{l}{No.of organs condemned \% of condemned price per organs}\tabcellsep Total price in ETB\\
\tabcellsep Lung\tabcellsep 200\tabcellsep 89\tabcellsep 20\tabcellsep 4000\\
\tabcellsep Liver\tabcellsep 20\tabcellsep 8.9\tabcellsep 50\tabcellsep 1000\\
\tabcellsep Kidney\tabcellsep 1\tabcellsep 0.45\tabcellsep 30\tabcellsep 30\\
\tabcellsep Spleen\tabcellsep 1\tabcellsep 0.45\tabcellsep 10\tabcellsep 10\\
\tabcellsep Heart\tabcellsep 1\tabcellsep 0.45\tabcellsep 30\tabcellsep 30\\
\tabcellsep Total\tabcellsep 223\tabcellsep 100\tabcellsep 140\tabcellsep 5070\end{longtable} \par
  {\small\itshape [Note: Direct economic loss from loss of organs condemned=NAS X ph [(plu x cplu) +(pli x cpli) +(psp x cpsp) +(phr x cphr) + (pkid x cpkid). NAS=Number of animals slaughtered annually in Jimma municipal abattoir were=9600]} 
\caption{\label{tab_5}Table 6 :}\end{figure}
 \begin{figure}[htbp]
\noindent\textbf{} \par 
\begin{longtable}{}
\end{longtable} \par
 
\caption{\label{tab_6}}\end{figure}
 			\footnote{© 2016 Global Journals Inc. (US)} 			\footnote{© 2016 Global Journals Inc. (US) Study on Prevalence and Monetary Loss Attributed to Hydatidosis in Cattle Slaughtered at Jimma Municipal Abattoir, Southwestern Ethiopia} 		 		\backmatter  			  				\begin{bibitemlist}{1}
\bibitem[ Ethiopia Vet. J]{b2}\label{b2} 	 		\textit{},  	 	 		\textit{Ethiopia Vet. J}  		11  (2)  p. .  	 
\bibitem[Harar]{b8}\label{b8} 	 		\textit{},  		 			Ethiopia Harar 		.  	 	 		\textit{Journal of Veterinary Medicine and Animal Health}  		7  (1)  p. .  	 
\bibitem[ European Journal of Applied Sciences]{b14}\label{b14} 	 		\textit{},  	 	 		\textit{European Journal of Applied Sciences}  		5  (1)  p. .  	 
\bibitem[Dar et al.]{b32}\label{b32} 	 		\textit{},  		 			Dar 		,  		 			North-Western 		,  		 			Ethiopia 		.  	 	 		\textit{East Mediterr Hlth J}  		16 p. .  	 
\bibitem[Trop]{b50}\label{b50} 	 		\textit{},  		 			Trop 		.  		113 p. .  	 
\bibitem[Urquhart and Armour]{b53}\label{b53} 	 		\textit{},  		 			G Urquhart 		,  		 			J Armour 		.  		 	 
\bibitem[ Global Journal of Medical Research]{b58}\label{b58} 	 		\textit{},  	 	 		\textit{Global Journal of Medical Research}  		 	 
\bibitem[Cyprus]{b44}\label{b44} 	 		‘93: 981-1992. regions in Ethiopia, an assessment trial on its prevalence, economic and public health importance’.  		 			Cyprus 		.  	 	 		\textit{Revue Méd. Vét}  		147 p. .  	 
\bibitem[Agricultural sample survey Report on livestock and livestock characteristics (private peasant holdings) ()]{b11}\label{b11} 	 		‘Agricultural sample survey’.  	 	 		\textit{Report on livestock and livestock characteristics (private peasant holdings)},  				 (Addis Ababa, Ethiopia)  		2012. 2011-2012. 446 p. 188.  		 			Central Statistical Agency 		 	 
\bibitem[Macpherson et al. ()]{b35}\label{b35} 	 		‘An Echinococcosis pilot control program for North West Turkana’.  		 			C N L Macpherson 		,  		 			E Zeyhele 		,  		 			T Roming 		.  	 	 		\textit{Ann.trop. med parasit}  		1985. 78 p. .  	 
\bibitem[Polydorou ()]{b43}\label{b43} 	 		\textit{Animal Health and Economics: Case Study of Echinococcosis with a reference to},  		 			F Polydorou 		.  		1981.  	 
\bibitem[De Lahunta and Habel ()]{b12}\label{b12} 	 		\textit{Applied veterinary anatomy},  		 			A De Lahunta 		,  		 			R E Habel 		.  		1986. W.B. Saunders Company. p. .  	 
\bibitem[Zewdu et al. ()]{b57}\label{b57} 	 		‘Bovine Hydatidosis in Ambo Municipality Abattoir, West Shoa, Ethiopia. Ethiop’.  		 			E Zewdu 		,  		 			T Yechale 		,  		 			M Assefa 		.  	 	 		\textit{Vet. J}  		2010. 14  (1)  p. .  	 
\bibitem[Belina et al. ()]{b7}\label{b7} 	 		\textit{Bovine hydatidosis: prevalence, public health and its economic significance in and around},  		 			D Belina 		,  		 			G Fekadu 		,  		 			E Zegaye 		,  		 			S Belina 		.  		2015.  	 
\bibitem[Mesele et al. ()]{b37}\label{b37} 	 		‘Cystic Echinococcosis of Cattle in Jimma Municipal Abattoir’.  		 			A Mesele 		,  		 			T Solomon 		,  		 			Sh Desie 		.  	 	 		\textit{South West Ethiopia. Global Veterinaria}  		2013. 11  (6)  p. .  	 
\bibitem[Dvm Thesis et al.]{b16}\label{b16} 	 		‘Echinococcosis, a Zoonosis of Increasing Concern’.  		 			, Dvm Thesis 		,  		 			Aau 		,  		 			Fvm 		,  		 			Ethiopia Dz 		.  	 	 		\textit{Clin. Microbio, Rev}  		17 p. .  	 
\bibitem[Fikre ()]{b22}\label{b22} 	 		\textit{Echinococcosis/Hydatidosis in Konso (Southern Ethiopia), An Assessment trial of its Prevalence, Economic and Public Health Importance},  		 			L Fikre 		.  		1994. FVM, AAU, DZ, Ethiopia.  	 	 (DVM Thesis) 
\bibitem[Kebede et al. ()]{b33}\label{b33} 	 		‘Echinococcosis/hydatidosis: its prevalence, economic and public health significance in Tigray region’.  		 			W Kebede 		,  		 			A Hagos 		,  		 			Z Girma 		,  		 			F Lobago 		.  	 	 		\textit{Trop. Anim. Hlth. and Prod}  		2009b. 41 p. .  	 
\bibitem[Eckert and Deplazes ()]{b17}\label{b17} 	 		 			J Eckert 		,  		 			P Deplazes 		.  		\textit{Biological, Epidemiological, and Clinical Aspects of},  				2004.  	 
\bibitem[Azlaf and Dakkak ()]{b6}\label{b6} 	 		‘Epidemiological study of the cystic echinococcosis in Morocco’.  		 			R Azlaf 		,  		 			A Dakkak 		.  	 	 		\textit{Vet. Parasitol}  		2006. 137 p. .  	 
\bibitem[Debela et al. ()]{b15}\label{b15} 	 		‘Epidemiology and Financial Loss of Bovine Hydatidosis inCattle Slaughtered at Nekemte Municipal Abattoir, Ethiopia’.  		 			A Debela 		,  		 			D Fanta 		,  		 			D Benti 		,  		 			M Nebyu 		,  		 			A Zerihun 		.  	 	 		\textit{Acta Parasitologica Globalis}  		2014. 5  (2)  p. .  	 
\bibitem[Tsehay ()]{b51}\label{b51} 	 		\textit{Epidemiology of Bovine Faciolosis and Hydatidosis in Debre-Brahan region},  		 			T Tsehay 		.  		1995. FVM, AAU, DZ, Ethiopia.  	 	 (DVM Thesis) 
\bibitem[Esatgil and Tuzer ()]{b18}\label{b18} 	 		 			M U Esatgil 		,  		 			E Tuzer 		.  		\textit{Prevalence of hydatidosis in slaughtered animals in Thrace, Turkey. Turkiye Parazitoloji Dergisi},  				2007. 31 p. .  	 
\bibitem[Fao ()]{b19}\label{b19} 	 		\textit{Food and Agriculture Organization of the United Nations Rome},  		 			Fao 		.  		1994.  	 	 (Manual on meat inspection for developing countries) 
\bibitem[Gemmel et al. ()]{b23}\label{b23} 	 		 			M A Gemmel 		,  		 			M G Roberts 		,  		 			T C Beard 		,  		 			S Campanods 		,  		 			J R Lawson 		,  		 			J M Nonnomaker 		.  		\textit{control of Echinococcosis, WHO/ OIE manual in Echinococcosis in humans and animals},  				2002. p. .  	 
\bibitem[Himonas ()]{b26}\label{b26} 	 		 			C Himonas 		.  		\textit{The Fertility of Hydatid cyst in Food Animals in},  				 (Greece, Helmenth, Zoonosis, Martin, Nijohoft, Publisher, Neitherland)  		1987. p. .  	 
\bibitem[Bersissa ()]{b9}\label{b9} 	 		\textit{Hydatidosis in Nekemte: prevalence in slaughtered cattle and Sheep, estimated economic loss and incidence in stray dogs},  		 			K Bersissa 		.  		1994. AAU, FVM, DZ, Ethiopia. p. 15.  	 	 (DVM Thesis) 
\bibitem[Nigatu et al. ()]{b42}\label{b42} 	 		\textit{Hydatidosis of slaughtered Cattle in walaita soddo abattoir, southern Ethiopia, Tropical animal health prod},  		 			K Nigatu 		,  		 			M W Habtamu 		,  		 			T Getachew 		.  		2009. 41 p. .  	 
\bibitem[Hagos ()]{b25}\label{b25} 	 		\textit{Hydatidosis/echinococcosis: prevalence and economic impact in bovine},  		 			Y Hagos 		.  		1997.  	 
\bibitem[Alemu and Yitagele ()]{b3}\label{b3} 	 		‘Hydatidosis: Prevalence, Cyst Distribution and Economic Significance in Cattle Slaughtered at Arbaminch Municipality Abattoir, Southern Ethiopia’.  		 			T Alemu 		,  		 			T Yitagele 		.  	 	 		\textit{Global Veterinaria}  		2013. 11  (3)  p. .  	 
\bibitem[Abebe and Yilma ()]{b0}\label{b0} 	 		‘Infectionprevalence of hydatidosis (Echinococcusgranulosus, Bats ch, 1786) in domestic animals in Ethiopia: Ethiop’.  		 			F Abebe 		,  		 			J Yilma 		.  	 	 		\textit{Vet. J}  		2011. 15  (2)  p. .  	 
\bibitem[Jobre et al. ()]{b28}\label{b28} 	 		 			Y Jobre 		,  		 			F Lobago 		,  		 			R G Tiruneh 		,  		 			P Dorchies 		.  		\textit{Hydatidosis in three selected},  				1996.  	 
\bibitem[Kebede et al. ()]{b31}\label{b31} 	 		 			N Kebede 		,  		 			A Mitiku 		,  		 			G Tilahun 		.  		\textit{Retrospective survey of human hydatidosis in Bahir},  				2010.  	 
\bibitem[Negassa et al. ()]{b40}\label{b40} 	 		‘Livestock production and marketing’.  		 			A Negassa 		,  		 			S Rashid 		,  		 			B Gebremedhin 		.  		 \url{http://www.ifpri.org/sites/default/files/publications/esspwp26.pdf}  	 	 		\textit{International food policy research institute},  				 (Addis Ababa, Ethiopia)  		2010.  	 
\bibitem[Moges ()]{b38}\label{b38} 	 		 			E Moges 		.  		\textit{Study on Bovine Faciolosis and Hydatidosis at Jimma abattoir},  				 (FVM, AAU, DZ, Ethiopia)  		2003.  	 	 (DVM Thesis) 
\bibitem[Mcmanus ()]{b36}\label{b36} 	 		\textit{Molecular discrimination of Hydatid cestodes, parasitaol Int},  		 			D P Mcmanus 		.  		2006. p. .  	 
\bibitem[Bizuwork et al. ()]{b10}\label{b10} 	 		‘Occurrences and financial significance of bovine cystic echinococcosis in Southern Wollo’.  		 			A Bizuwork 		,  		 			G Kebede 		,  		 			T Tibat 		,  		 			G Tilahun 		,  		 			T Kassa 		.  	 	 		\textit{Northeastern Ethiopia Journal of Veterinary Medicine and Animal Health}  		2013. 5  (2)  p. .  	 
\bibitem[Zelalem ()]{b56}\label{b56} 	 		‘prevalence and economic impact of hydatidosis in Addis Ababa abattoir’.  		 			F Zelalem 		.  	 	 		\textit{JUCAVM}  		2008. p. 38.  		 			School of veterinary Medicine 		 	 	 (DVM Thesis) 
\bibitem[Umur ()]{b52}\label{b52} 	 		‘Prevalence and Economic Importance of cystic Echinococcosis in Slaughtered Ruminants in Burdur’.  		 			S Umur 		.  	 	 		\textit{Turkey. J. Vet. Med}  		2003. 50 p. .  	 
\bibitem[Debas and Ibrahim ()]{b13}\label{b13} 	 		\textit{Prevalence and Economic Importance of Hydatidosis inCattle Slaughtered at North Gonder Elfora Abattoir},  		 			E Debas 		,  		 			N Ibrahim 		.  		2013.  	 
\bibitem[Kebede et al. ()]{b30}\label{b30} 	 		\textit{Prevalence and Financial Effects of Hydatidosis in Cattle Slaughtered in Birre-Sheleko and Dangila Abattoirs, Northwestern Ethiopia},  		 			N Kebede 		,  		 			Z Gebre-Egziabher 		,  		 			G Tilahun 		,  		 			A Wossene 		.  		2009a. Zoonoses Public Hlth. 58 p. .  	 
\bibitem[Terefe et al. ()]{b47}\label{b47} 	 		‘Prevalence and financial loss estimation of hydatidosis of cattle slaughtered at Addis Ababa abattoirs enterprise’.  		 			D Terefe 		,  		 			K Kebede 		,  		 			D Beyene 		,  		 			A Wondimu 		.  	 	 		\textit{Journal of Veterinary Medicine and Animal Health}  		2012. 4  (3)  p. .  	 
\bibitem[Koskei ()]{b34}\label{b34} 	 		\textit{Prevalence and strain differentiation of Echinococcus granulosus in some selected sites of Ethiopia},  		 			P K Koskei 		.  		1998.  		 			Berlin and Ethiopia: Freie University and Addis Ababa University 		 	 	 (MSc Thesis) 
\bibitem[Tolossa et al. ()]{b49}\label{b49} 	 		‘Prevalence of bovine cysticercosis and hydatidosis in Jimma municipal abattoir, SouthWest Ethiopia’.  		 			T Tolossa 		,  		 			W Tigre 		,  		 			G Teka 		,  		 			P Domy 		.  	 	 		\textit{Onderstepoort J. Vet. Res}  		2009. 76 p. .  	 
\bibitem[Abunna et al. ()]{b1}\label{b1} 	 		\textit{Prevalence of bovine hydatidosis in Kombolcha ELFORA abattoir},  		 			F Abunna 		,  		 			S Fentaye 		,  		 			B Megersa 		,  		 			A Regassa 		.  		2012. North Eastern.  	 
\bibitem[Woubet ()]{b55}\label{b55} 	 		\textit{Prevalence of cattle hydatidosis and its economic significance in Bale Robe municipal abattoir},  		 			S Woubet 		.  		1988.  	 
\bibitem[Getaw et al. ()]{b24}\label{b24} 	 		‘Prevalence of Hydatidosis and its economic importance in ruminants slaughtered at Adama municipal Abattoir, Central Oromia’.  		 			A Getaw 		,  		 			D Beyene 		,  		 			D Ayana 		,  		 			B Megersa 		,  		 			F Abunna 		.  	 	 		\textit{Acta Tropica}  		2010. 113 p. .  	 
\bibitem[Arene ()]{b5}\label{b5} 	 		\textit{Prevalence of hydatidosis in domestic livestock in the Niger Delta},  		 			F O I Arene 		.  		1985. p. .  	 	 (at Mekele Municipal abattoir, zoonosis and infection in dogs (Mekele Tigray) 
\bibitem[Arene ()]{b4}\label{b4} 	 		‘Prevalence of hydatidosis in domestic livestock in the Niger Delta’.  		 			F A I Arene 		.  	 	 		\textit{Trop. Anim. Health Prod}  		1995. 17 p. .  	 
\bibitem[Feyissa ()]{b21}\label{b21} 	 		\textit{Prevalence of hydatidosis in Nekemte municipal slaughter house},  		 			R Feyissa 		.  		1987. FVM, AAU, DZ, Ethiopia.  	 	 (DVM Thesis) 
\bibitem[Soulsby ()]{b46}\label{b46} 	 		 			E J Soulsby 		.  		\textit{Helminths/Arthropods and Protozoa of domesticate animals},  				 (London, Bailliere Tindall)  		1982. p. .  	 
\bibitem[Ibrahim ()]{b27}\label{b27} 	 		‘Study of cystic echinococcosis in slaughtered animals in Al Baha Region, Saudi Arabia, Interaction between some biotic and abiotic factors’.  		 			M M Ibrahim 		.  	 	 		\textit{Acta}  		2010.  	 
\bibitem[Nebiyou ()]{b39}\label{b39} 	 		\textit{Study of hydatidosis/ Echinococcosis in cattle slaughtered at Bahir-Dar Municipal Abattoir},  		 			G Nebiyou 		.  		1990. FVM, AAU, DZ, Ethiopia.  	 	 (DVM. Thesis) 
\bibitem[Kebede ()]{b29}\label{b29} 	 		\textit{Study on prevalence, Economic and Public Health importance of bovine hydatidosis in slaughtered Animals at Addis Ababa abattoir},  		 			E Kebede 		.  		2009. Ethiopia, Msc Thesis, AAU, FVM, DZ, Ethiopia. p. .  	 
\bibitem[Solomon ()]{b45}\label{b45} 	 		‘Study on the prevalence and Economic significance of Hydatidosis in Cattle slaughtered at Addis Ababa Municipal Abattoir Ethiopia, DVM Thesis, School of veterinary medicine’.  		 			G Solomon 		.  	 	 		\textit{JUCAVM}  		2011. p. 18.  	 
\bibitem[Feyesa et al. ()]{b20}\label{b20} 	 		‘Study on the prevalence of cystic hydatidosis and its economic significance in cattle slaughtered at Hawassa Municipal abattoir’.  		 			R Feyesa 		,  		 			M Alemante 		,  		 			B Jemere 		.  	 	 		\textit{Ethiopia. Trop. Anim. Heal. Prod}  		2010. 42 p. .  	 
\bibitem[Nicholson and Butterworth ()]{b41}\label{b41} 	 		‘The Guide to Body Condition Scoring of zebu Cattle’.  		 			M J Nicholson 		,  		 			M H Butterworth 		.  	 	 		\textit{International livestock Center for Africa}  		1986.  	 
\bibitem[Thrusfield ()]{b48}\label{b48} 	 		 			M Thrusfield 		.  		\textit{Veterinary Epidemiology},  				 (Oxford, Black Well Science, Ltd., 2)  		2005. p. 88.  	 
\bibitem[Duncan et al. ()]{b54}\label{b54} 	 		\textit{Vet parasitology 2 nd edn},  		 			J L Duncan 		,  		 			A M Dunn 		,  		 			F W Jennings 		.  		1996. 122 p. .  		 			Black well science Ltd UK 		 	 
\end{bibitemlist}
 			 		 	 
\end{document}
