# Introduction tability (sustainability) is a factor in the quality of medicines. The criterion for the stability of a medicinal substance is the preservation of its quality, i.e. appearance, solubility, authenticity, good quality and quantitative content. The decrease in the quantitative content of the pharmacologically active substance in the drug confirms its instability. The decrease in the quantitative content of the drug by 10% should not occur within 3-4 years in the finished dosage forms. To increase stability, chemical processes occurring during storage of drugs are investigated and methods are created to inhibit these processes. The solution of these problems is possible only on the basis of the development of methods for analyzing medicinal substances in the presence of their decomposition products. The results of these studies are taken into account when developing the technology of obtaining drugs and ND. Products of organic synthesis make up a significant part of the arsenal of medicines of modern medicine. Despite the significant number of drugs used in medical practice, the search for more effective and safe ones is constantly underway. Largely, this refers to groups of drugs used to treat inflammatory processes caused by bacterial microflora [1,2]. # II. # Material and Methods Material of the study is the substance "Intralin" -(cefazolin sodium salt/5-thia-1-azabicyclo [4.2.0] octa-2-Author ? ? ? ?: Tashkent Pharmaceutical Institute, Tashkent, Uzbekistan. e-mail: author.uzb@mail.ru en-2-carboxy, 3-[[(5-methyl-1,3,4-thiadiazole-2-yl) thio] methyl]-8-oxo-7-[[(1H-tetrazole-1-yl) acetyl] amino]-, sodium salt (?Y-trans)-) 0.5 or 1,0 g, powder for the preparation of injection solutions released by JV "Jurabek Laboratories" LLC. The experiments were carried out on 3 series of antibiotics obtained in the laboratory by the methods. All samples of antibiotics were pre-analyzed in accordance with the requirements of the FS project developed by the author. In determining the expiration dates, they were guided by the requirements of the Global Fund XI and the Interim Instruction I-42-2-82. According to these documents, the establishment of stability is possible using the following methods: ? Test under long-term storage conditions; ? Testing under conditions of "accelerated aging" according to the Interim Instruction I 42-2-82. The method of "accelerated aging", based on the law of van't Hoff, establishes the relationship between the shelf life of the substance and storage temperature ?? = ?? ?????????? 10 ??? ? ?????? 10 Where, experimental substance series: t? is the temperature of experimental storage. t xp -storage temperature. Investigations were carried out on 3 series of cefazolin substance (040113, 070113, 100113) at an experimental storage temperature of 60 º?. Samples were placed in vials of 0.5 or 1.0 g of the drug from a glass tube of the type FO-1-15 or 00-1-20 according to TU 64-2-10-87 or imported in accordance with ISO 8362-1 and ISO 8362-4, hermetically sealed with stoppers of rubber stamps I-51ili 52-599 / 1, or IR-119po TU 38.006108-90 or imported according to ISO 8362-2, compressed with aluminum caps of type K-2, as per ISO 6462-686 or imported according to ISO 8362-6. Quality control was carried out at time intervals (11.5 days), equivalent to 6 months of storage in natural conditions according to the indicators given in table 1 [4,5]. The results of the experiment (Table 2) showed that the cefazolin substance remains stable for 69 days of experimental storage, which corresponds to 1104 days of storage under natural conditions, calculated according to the van't-Hoff rule: compliance rate. Hence, the shelf life, which is 3 years. Storage temperature, which allows to ensure the established shelf life is: The maximum allowable storage temperature is: Thus, as a result of the studies conducted by the method of "accelerated aging", the shelf life and temperature storage of the cefazolin substance has been established. Thus, a preliminary shelf life of 3 years can be established. The study of the stability of cefazolin was also carried out by the method of long-term storage. For this, cefazolin series 151210, 171210, and 201210 were stored in dry and dark place that ground-glass jars with ground glass stoppers at room temperature. Quality control was carried out on the main indicators, at intervals equal to 1 year of storage. Studies (tab. 3) showed that the stability of the substance cefazolin in long-term storage conditions is maintained for at least 3 years. III. # Conclusion 1. It was found that the stability and shelf life of the investigated drug, established by the method of "accelerated aging" at a temperature of 60 °C, is not less than 3 years, and the storage temperature is from 20 to 26 °C. 2. It is established that the substance of cefazolin in the conditions of long-term storage at room temperature in a dark place is not less than 3 years. 1IndicatorsMethodsStandardDescriptionVisualPowder white or almost white, very hygroscopicSolubilityVisualEasily soluble in water, very little soluble in 96% alcohol1. IR absorption spectrum of drug sample obtained in disks withInfrared spectrometrypotassium bromide (about 2 mg of the drug in 200 mg of potassium bromide) from 4000 to 450 cm "should correspond to the spectrum ofthe RSO cefazolin sodium salt (about 2 mg of the RSO cefazolinAuthenticitysodium salt in 200 mg of potassium bromide).UV Spectrophotometer2. The ultraviolet absorption spectrum of the solution in the region from 220 to 350 nm should have an absorption maximum at a wavelength of 272+2 nm. Chemical reactions 3. The drug gives a characteristic reaction to sodium. Quantitative content HPCL From 850 to 1050 mcg, calculated on the dry matter 2SeriesLife expectancy, in daysDescriptionIR-spectrumAuthenticity UV spectrumCharacteristic reaction to sodiumQuantitative content, %12345670White or almost white crystalline odorless powder;The spectrum RSO cefazolin corresponds to the spectrum ofMaximum at 272+2 nm.Yellow precipitate99,911,5-// --// --// --// -99,8504011323-// --// --// --// -99,8634,5-// --// --// --// -99,8446-// --// --// --// -99,9657,5-// --// --// --// -99,7569-// --// --// --// -99,710White or almost white crystalline odorless powder;The spectrum cefazolin RSO corresponds to the spectrum ofMaximum at 272 + 2 nm.Yellow precipitate99,9511,5-// --// --// --// -99,9707011323-// --// --// --// -99,8534,5-// --// --// --// -99,8446-// --// --// --// -99,8557,5-// --// --// --// -99,7269-// --// --// --// -99,700White or almost white crystalline odorless powder;The spectrum cefazolin RSO corresponds to the spectrum ofMaximum at 272+2 nm.Yellow precipitate99,9010011311,5-// --// --// --// -99,8523-// --// --// --// -99,8234,5-// --// --// --// -99,9146-// --// --// --// -99,9357,5-// --// --// --// -99,7569-// --// --// --// -99,72 3SeriesExpiry date, in yearsDescriptionIR spectrumAuthenticity UF spectrumcharacteristicre actiontosodiumQuantitative content, %1234567White or almost white crystalline powder;The spectrum corresponds to the spectrum ofMaximum at 272+2 nm.Yellow precipitate99,40151210odorlesscefazolin RSO1-// --// --// --// -99,812-// --// --// --// -99,793-// --// --// --// -99,720White or almost white crystalline powder;The spectrum corresponds to the spectrum ofMaximum at 272+2 nm.Yellow precipitate99,81171210odorlesscefazolin RSO1-// --// --// --// -99,782-// --// --// --// -99,733-// --// --// --// -99,69White or almostThe spectrum0white crystalline powder;corresponds to the spectrum ofMaximum at 272+2 nm.Yellow precipitate99,87201210without smellcefazolin RSO1-// --// --// --// -99,812-// --// --// --// -99,783-// --// --// --// -99,75 * Recommendations of the World Health Organization for the study of the stability of reproducible pharmaceutical products. Farmateka, ? 6/95 APMeshkovsky * The problem of stability and shelf life of drugs is relevant for Russia. Farmateka, ? 1/96 MMashkovsky 38 * About the recommendations of the WHO for the study of the stability of the reproduced pharmaceutical products. Farmateka, ? 1/96 IPRudakova * Dusmatov Development and validation of the wet mass spectroscopy method in the analysis of the INTRALIN drug RAKhusainova KAUbaidullaev AF Journal of Pharmacy 2/2017 * In accordance with modern requirements, in accordance with the General technical regulation on