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\title{Comparison between Threshold of Bitterness Perception and Blood Pressure for Resident Health Examination in Yakumo Town}
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             \author[1]{Naomi  Katayama}

             \author[2]{Akemi  Ito}

             \author[3]{Mayumi  Hirabayashi}

             \author[4]{Shoko  Kondo}

             \author[5]{Yui  Nakayama}

             \author[6]{Takafumi  Nakada}

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\date{\small \em Received: 11 December 2019 Accepted: 4 January 2020 Published: 15 January 2020}

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\begin{abstract}
        


Japan is a super-aged society. Malnutrition, sarcopenia, and flail in the elderly are problems. It has also reported that abnormal olfaction and taste (function decline) occur as an early symptom of Alzheimer?s dementia. Taste and smell have a close relationship with appetite. It is need to study the provision of meals that enhance and the combination of foods from the middle age to the elderly. Because Alzheimer?s dementia gradually progresses from the middle-ages, and symptoms spear after becoming an older adult. For 16 years, we have conducted research on taste and olfaction in Yakumo town in Hokkaido, Japan, Where the population does not move much. In this report, we report on the results of the taste test using TASTDISC (Bitterness) in 2019 at Yakumo Town Resident Examination, which has been ongoing since 2007.

\end{abstract}


\keywords{bitterness; taste; gender; healthy older adult; yakumo study.}

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\let\tabcellsep& 	 	 		 \par
sarcopenia, and flail in the elderly are problems. It has also reported that abnormal olfaction and taste (function decline) occur as an early symptom of Alzheimer's dementia. Taste and smell have a close relationship with appetite. It is need to study the provision of meals that enhance and the combination of foods from the middle age to the elderly. Because Alzheimer's dementia gradually progresses from the middle-ages, and symptoms spear after becoming an older adult. For 16 years, we have conducted research on taste and olfaction in Yakumo town in Hokkaido, Japan, Where the population does not move much. In this report, we report on the results of the taste test using TASTDISC (Bitterness) in 2019at Yakumo Town Resident Examination, which has been ongoing since 2007.From the database, 298 participants (169 females and 129 males) were selected form data in August 2019. The bitterness test performed using the bitterness test paper with liquid TASTEDISC (Sanwa Chemical Laboratory Co., Ltd) which include five different densities of Quinine hydrochloride on a liquid with test paper namely:, 1(0.001\%), 2(0.02\%),3(0.1\%), 4(0.5\%), 5(4.0\%). As a result, 12males out of 129 male participants (9.3\%) and 9females of 169 female participants (5.3\%) had abnormal values in bitter taste test (Taste disc) results. Feeling bitterness can also protect us from ingesting dangerous foods (various toxins). However, a low threshold of bitterness in important toenjoy the taste of spring vegetables as such as wild vegetables and the delicious beer taste. Bitterness studies are increasingly needed. 
\section[{Introduction}]{Introduction}\par
apan is aging and has become a super-aged society as of 2020. The Japanese government is working with prefectures to take measures to extend healthy life expectancy, aiming for healthy longevity. We are recruiting participants for various events, such as cooking classes for preventing under nutrition of the elderly, exercise for preventing dementia, and cooking classes. Taste mainly studied for saltiness and sweetness. This is because the salty taste is closely related to cardiovascular areas such as blood pressure, and the government calls for salt reduction from the perspective of preventing hypertension. Also, the sweetness is related to blood sugar level, HbA1c, etc. It is involved in obesity, diabetes, and even Alzheimer's dementia and a great deal of research has reported to improve lifestyle-related diseases. However, the taste has sourness, bitterness, and umami. Therefore, this study tries to understand what is the threshold value of the bitter taste that determines the taste of beer from the middle ages to the elderly. The bitterness is food is known as a poison such as an alkaloid mainly contained in vegetables. A small amount of bitter taste, like spices, can change the taste of a meal and help to create a variety of tastes. The best example is "the hops" which are indispensable for making beer. At first, even if there is resistance to bitterness, it is that we will want to repeat or eat it. We get used to it. A low threshold of bitterness to quickly detect dangerous tastes and avoid poisonous foods and drinks. From the above, it is also necessary to study the threshold of bitterness that decline with age. This time, we will report the results of a bitterness threshold test conducted at the time of resident screening in Yakumo Town in Hokkaido, Japan, where the population does not move much. 
\section[{II.}]{II.} 
\section[{Materials and Methods}]{Materials and Methods} 
\section[{a) Participants}]{a) Participants}\par
Yakumo is located in the south of Hokkaido, the northern island of Japan. Townspeople make a living mainly in agriculture and fisheries. The study in Yakumo is a prospective cohort study. This research has been ongoing since 1981. The reason is that Yakumo Town has the least migration of population in Japan. The participants had managed their everyday life themselves. And the Nagoya University Graduate School of Medicine, professionals in the fields of epidemiology, internal medicine, orthopedics, neuropsychology, ophthalmology, otolaryngology, and urology joined the Yakumo Study. The participants had been engaged in a variety of jobs. Therefore, this town can be regarded as representative of today's Japanese society. From the database, 298participants (169 females and 129 males) were selected form data in August 2019 (Table \hyperref[tab_0]{1}). 
\section[{b) Assessment of bitter taste identification}]{b) Assessment of bitter taste identification}\par
The gustatory test was performed using test paper with liquid TASTEDISC (Sanwa Chemical Laboratory Co., Ltd) which include five different densities of Quinine hydrochloride on a liquid with test paper namely:, 1(0.001\%), 2(0.02\%),3(0.1\%), 4(0.5\%), 5(4.0\%).The inspection method is as follows. 1) Show participants the taste choice paper: Sweet, Salty, Sour, Bitter. Taste something but I don't know, No taste. 2) Hold the filter paper disc with tweezers. The bitterening solution is dropped on it and moistened. 3) The moistened disc is gently placed on the canaliculus chordae tympani innervation area of the participant's tongue. The canaliculus chordaetympani innervation area is located 2 cm left and right from the tip of it. 4) Instruct the user to answer one of the taste choice paper in 2\textasciitilde 3 seconds with the mouth open. 5) The examiner then removes the disc from the participant's tongue with tweezers. 6) If a correct answer is not obtained to the participants, the test is continuing to use a solution having a higher concentration in order. 7) After garglling with water to prevent residual teste, perform the next taste test at intervals of 1 minute or more. This method was in accordance with the test method of the teste test kit (TASTEDISC: Sanwa Chemical Laboratory Co., Ltd). 
\section[{c) Ethical review board}]{c) Ethical review board}\par
This study is conducted with the approval of the Ethical Review Board (Nagoya women's university Ethics Committee: 'hito wo mochiita kennkyuu ni kansuru iinnkai'). The approval number is 30-14. 
\section[{d) Statistical processing}]{d) Statistical processing}\par
The test results were confirmed to be normal distribution by F-test. Data that werethe tolerance range (in this study, it called the normal range) distributed were compared with Student-t without correlation of parametric test. The data that is not normally distributed was compared without correlated Mann-Whitney one of the non-parametric test. 
\section[{III.}]{III.} 
\section[{Results}]{Results} 
\section[{a) Participant's body composition and blood pressure}]{a) Participant's body composition and blood pressure}\par
Data on body composition and blood pressure of participants show by age. The males showed in Table \hyperref[tab_1]{2}. And the females showed in Table \hyperref[tab_2]{3}. All data showed as averages by age. For both males and females, the mean values of blood pressure for each generation were in the normal range. Body fat percentage was higher in females than in males, and BMI and body fat were almost the tolerance range (in this study, it called the normal range) for both males and females.    
\section[{b) Assessment of bitter taste identification}]{b) Assessment of bitter taste identification}\par
Bitter taste identification performed by using test paper TASTDESC (Sanwa Chemical Laboratory Co., Ltd).Table \hyperref[tab_3]{4} shows the bitterness measurement results for male and female by age. The bitterness results using TASTDISC can teste for sensitivity to Quinine hydrochloride concentrations. As a result, 12 males of 129 male participants (9.3\%) and nine females of 169 female participants (5.3\%) had abnormal values in the result of bitterness test. Males almost twice as many as females required consultation. 
\section[{c) Statistical processing results}]{c) Statistical processing results}\par
The bitter test result was statistically processed. Table \hyperref[tab_4]{5} and Table \hyperref[tab_5]{6} show the results of comparison of the bitterness test results using TASTDISC with the tolerance range (in this study, it called the normal range) systolic and diastolic blood pressure values and others. The results did not show a statistically significant difference in either case.    \hyperref[tab_6]{7} shows the results of the comparison of the bitterness test results using TASTDISC with the tolerance range (in this study, it called the normal range) BMI and others. The results did not show a statistically significant difference in either case.\par
Table \hyperref[tab_7]{8} shows the results of the comparison of the bitterness test results using TASTDISC with the tolerance range (in this study, it called the normal range) Body fat and others. The results showed that there was a statistically significant difference between the tolerance range (in this study, it called the normal range)    \hyperref[tab_11]{10} shows the results of the comparison of the bitterness test results using TASTDISC with the tolerance range (in this study, it called the normal range) Waist circumference range and In Japan, the tolerance range (in this study, it called the normal range) waist circumference of the male is less than 85 cm (Table \hyperref[tab_8]{9}), and female is less than 90 Cm (Table10). The results did not show a statistically significant difference in either case.    \hyperref[tab_11]{10}). The results did not show a statistically significant difference in either case.\par
IV. 
\section[{Discussion}]{Discussion}\par
The taste is mainly divided into 5 flavors, sweetness, saltiness, sourness, bitterness, and umami \hyperref[b0]{1,}\hyperref[b1]{2,}\hyperref[b2]{3,}\hyperref[b3]{4)} . It has reported that the cognitive threshold of taste changes with temperature \hyperref[b4]{5)} . Of these five tastes, saltiness and bitterness are to be thin when the temperature is high when the temperature is low \hyperref[b5]{6)} . Many medicines have a bitter taste and may be difficult for children to take, especially \hyperref[b6]{7)} . Many researchers have reported the results of studies of coating the bitter drug with another taste \hyperref[b7]{8)} . However, bitterness is an ability necessary to identify dangerous things (poisonous substances, etc.) \hyperref[b8]{9)} . The problem is that with age, the taste may deteriorate, and we may not feel the taste \hyperref[b9]{10)} . Particularly today, it has begun to be reported that taste perception due to Alzheimer's dementia deteriorates \hyperref[b10]{11,}\hyperref[b11]{12,}\hyperref[b12]{13)} . Furthermore, it has reported that patients were suffering from coronavirus report sysgeusia \hyperref[b13]{14,}\hyperref[b14]{15)} . It can say that it is need to study taste. According to the results of our previous research on saltiness, there was no statistically significant difference in saltiness threshold results between all normal ranges, such as blood pressure, BMI, body fat percentage, waist circumference, and the other values \hyperref[b14]{15)} . However, this time, when comparing bitterness with the normal range of blood pressure, BMI, body fat percentage, waist circumference as in the case of salty taste, there was a statistically significant difference in body fat percentage. Participants with a low body fat percentage had a high threshold of bitterness, and participants with abundant body fat percentage had a high sensitivity of bitterness. Regarding this result, we need to further investigate. And the relationship between diet and other factors which we need to study in the future.\par
V. 
\section[{Conclusions}]{Conclusions}\par
We obtained bitterness test results, TASTDISC, at the time of health check-up in Yakumo Town, Hokkaido, where population migration is low. From the database, 298 participants (169 females and 129 males) were selected form data in August 2019. The Bitterness test performed using test paper with liquid TASTEDISC (Sanwa Chemical Laboratory Co., Ltd) which include five different densities of Quinine hydrochloride on a liquid with test paper namely:, 1(0.001\%), 2(0.02\%), 3(-.1\%), 4(0.5\%), 5(4.0\%). As a result, 12 males out of 129 male participants (9.3\%) and nine females of 169 female participants (5.3\%) had abnormal values in the bitterness taste test (TASTEDISC) results. The tolerance range (in this study, it called the normal range) of bitterness, blood pressure (systole, diastole), and body composition (BMI, body fat percentage, abdominal circumference) compared with other values. As a result, there was no statistically significant difference in the bitterness threshold between the normal range of blood pressure, BMI, and abdominal circumference and the others. However, there was a statistically significant difference in the threshold of bitterness between the normal range of body fat percentage and the other range. Bitterness thresholds were lower in participants with high body fat than in participants with low body fat. It is necessary to increase the number of participants and analyse it in the future.\begin{figure}[htbp]
\noindent\textbf{1} \par 
\begin{longtable}{P{0.4112903225806452\textwidth}P{0.0913978494623656\textwidth}P{0.0913978494623656\textwidth}P{0.10053763440860215\textwidth}P{0.0913978494623656\textwidth}P{0.06397849462365592\textwidth}}
Participants\tabcellsep 40's\tabcellsep 50's\tabcellsep 60's\tabcellsep 70's\tabcellsep 80's\\
Male (129)\tabcellsep 10\tabcellsep 24\tabcellsep 49\tabcellsep 40\tabcellsep 6\\
Female (169)\tabcellsep 23\tabcellsep 40\tabcellsep 66\tabcellsep 37\tabcellsep 3\\
Total (298)\tabcellsep 33\tabcellsep 64\tabcellsep 115\tabcellsep 77\tabcellsep 9\end{longtable} \par
 
\caption{\label{tab_0}Table 1 :}\end{figure}
 \begin{figure}[htbp]
\noindent\textbf{2} \par 
\begin{longtable}{}
\end{longtable} \par
 
\caption{\label{tab_1}Table 2 :}\end{figure}
 \begin{figure}[htbp]
\noindent\textbf{3} \par 
\begin{longtable}{P{0.3387857142857143\textwidth}P{0.014571428571428572\textwidth}P{0.032785714285714286\textwidth}P{0.08985714285714286\textwidth}P{0.03764285714285714\textwidth}P{0.08135714285714285\textwidth}P{0.04007142857142857\textwidth}P{0.08742857142857142\textwidth}P{0.06678571428571428\textwidth}P{0.06071428571428571\textwidth}}
\multicolumn{2}{l}{Number}\tabcellsep Age\tabcellsep Height\tabcellsep Weight\tabcellsep Waist\tabcellsep BMI\tabcellsep Body fat\tabcellsep Systolic\tabcellsep Dyastolic\\
\tabcellsep \tabcellsep \tabcellsep \tabcellsep \tabcellsep \tabcellsep \tabcellsep rate\tabcellsep blood\tabcellsep blood\\
\tabcellsep \tabcellsep \tabcellsep \tabcellsep \tabcellsep \tabcellsep \tabcellsep \tabcellsep pressure\tabcellsep pressure\\
\tabcellsep \tabcellsep \tabcellsep cm\tabcellsep g\tabcellsep cm\tabcellsep kg/m/m\tabcellsep \%\tabcellsep mmHg\tabcellsep mmHg\\
Average of 40's Female\tabcellsep 23\tabcellsep 45.2\tabcellsep 158.0\tabcellsep 57.2\tabcellsep 76.7\tabcellsep 22.8\tabcellsep 33.2\tabcellsep 122.3\tabcellsep 70.1\\
Average of 50's Female\tabcellsep 40\tabcellsep 54.3\tabcellsep 155.5\tabcellsep 56.4\tabcellsep 76.8\tabcellsep 23.3\tabcellsep 33.4\tabcellsep 131.6\tabcellsep 77.4\\
Average of 60's Female\tabcellsep 66\tabcellsep 64.5\tabcellsep 153.8\tabcellsep 55.7\tabcellsep 77.7\tabcellsep 23.5\tabcellsep 33.9\tabcellsep 137.1\tabcellsep 77.0\\
Average of 70's Female\tabcellsep 37\tabcellsep 72.8\tabcellsep 150.6\tabcellsep 52.8\tabcellsep 76.2\tabcellsep 23.3\tabcellsep 33.1\tabcellsep 140.1\tabcellsep 74.7\\
Average of 80's Female\tabcellsep 3\tabcellsep 82.0\tabcellsep 147.4\tabcellsep 49.6\tabcellsep 78.1\tabcellsep 22.9\tabcellsep 31.1\tabcellsep 149.0\tabcellsep 77.0\\
Total average of Female\tabcellsep 169\tabcellsep 61.6\tabcellsep 154.0\tabcellsep 55.3\tabcellsep 77.0\tabcellsep 23.3\tabcellsep 33.4\tabcellsep 134.7\tabcellsep 75.7\\
\multicolumn{2}{l}{(n=298)}\tabcellsep \tabcellsep \multicolumn{2}{l}{Normal 0.001\%, 0.02\%}\tabcellsep \multicolumn{2}{l}{Observation 0.1\%, 0.5\%}\tabcellsep \multicolumn{2}{l}{Consultation 4.0\% ormore}\tabcellsep \\
\multicolumn{2}{l}{Male 40's (n=10)}\tabcellsep \tabcellsep 4\tabcellsep \tabcellsep 4\tabcellsep \tabcellsep 2\tabcellsep \tabcellsep \\
\multicolumn{2}{l}{Male 50's (n=24)}\tabcellsep \tabcellsep 11\tabcellsep \tabcellsep 11\tabcellsep \tabcellsep 2\tabcellsep \tabcellsep \\
\multicolumn{2}{l}{Male 60's (n=49)}\tabcellsep \tabcellsep 27\tabcellsep \tabcellsep 17\tabcellsep \tabcellsep 5\tabcellsep \tabcellsep \\
\multicolumn{2}{l}{Male 70's (n=40)}\tabcellsep \tabcellsep 23\tabcellsep \tabcellsep 15\tabcellsep \tabcellsep 2\tabcellsep \tabcellsep \\
Male 80's (n=6)\tabcellsep \tabcellsep \tabcellsep 2\tabcellsep \tabcellsep 3\tabcellsep \tabcellsep 1\tabcellsep \tabcellsep \\
\multicolumn{2}{l}{Male total (n=129)}\tabcellsep \tabcellsep 67\tabcellsep \tabcellsep 50\tabcellsep \tabcellsep 12\tabcellsep \tabcellsep \\
\multicolumn{2}{l}{Female 40's (n=23)}\tabcellsep \tabcellsep 16\tabcellsep \tabcellsep 7\tabcellsep \tabcellsep 0\tabcellsep \tabcellsep \\
\multicolumn{2}{l}{Female 50's (n=40)}\tabcellsep \tabcellsep 32\tabcellsep \tabcellsep 8\tabcellsep \tabcellsep 0\tabcellsep \tabcellsep \end{longtable} \par
 
\caption{\label{tab_2}Table 3 :}\end{figure}
 \begin{figure}[htbp]
\noindent\textbf{4} \par 
\begin{longtable}{P{0.21364864864864863\textwidth}P{0.1768918918918919\textwidth}P{0.057432432432432436\textwidth}P{0.2963513513513513\textwidth}P{0.10567567567567568\textwidth}}
\tabcellsep \multicolumn{2}{l}{Systolic biood pressure (mmHg)}\tabcellsep \multicolumn{2}{l}{Tastedisc test result (Normal=1, Ovservation = 2, Consultation =3)}\\
\tabcellsep Less than 120\tabcellsep 120 or more\tabcellsep Systolic blood pressure? Less than 120\tabcellsep Systolic blood pressure 120 or more\\
Average±Standard deviaton\tabcellsep 108.934±19.003\tabcellsep 144.616±16.889\tabcellsep 1.459±0.601\tabcellsep 1.435±0.625\\
? test\tabcellsep \multicolumn{2}{l}{P=0.0001**}\tabcellsep \multicolumn{2}{l}{P=0.326}\\
Unpaired student-ï½?" test\tabcellsep \tabcellsep \tabcellsep \multicolumn{2}{l}{P=0.768}\\
Mann-Whaitny test\tabcellsep \multicolumn{2}{l}{P=0.0001**}\tabcellsep \tabcellsep \\
* P<0.05, ** P<0.01\tabcellsep \tabcellsep \tabcellsep \tabcellsep \end{longtable} \par
 
\caption{\label{tab_3}Female 60's (n=66) 45 16 5 Female 70's (n=37) 23 10 4 Female 80's (n=3) 3 0 0 Female total(n=169) 119 41 9 TastediscTable 4 :}\end{figure}
 \begin{figure}[htbp]
\noindent\textbf{5} \par 
\begin{longtable}{}
\end{longtable} \par
 
\caption{\label{tab_4}Table 5 :}\end{figure}
 \begin{figure}[htbp]
\noindent\textbf{6} \par 
\begin{longtable}{P{0.2173813169984686\textwidth}P{0.17052067381317\textwidth}P{0.059877488514548244\textwidth}P{0.30719754977029096\textwidth}P{0.0950229709035222\textwidth}}
\tabcellsep \multicolumn{2}{l}{Diastolic blood pressure (mmHg)}\tabcellsep \multicolumn{2}{l}{Tastedisc test result (Normal=1, Ovservation = 2, Consultation =3)}\\
\tabcellsep Less than 90\tabcellsep 90 or more\tabcellsep Diastolic blood pressure Less than 90\tabcellsep Diastolic blood pressure 90 or more\\
Average±Standard deviaton\tabcellsep 73.984±8.903\tabcellsep 97.180±8.329\tabcellsep 1.417±0.605\tabcellsep 1.549±0.673\\
? test\tabcellsep P=0.291\tabcellsep \tabcellsep \multicolumn{2}{l}{P=0.183}\\
Unpaired student-ï½?" test\tabcellsep \multicolumn{2}{l}{P=0.0001**}\tabcellsep \multicolumn{2}{l}{P=0.615}\\
Mann-Whaitny test\tabcellsep \tabcellsep \tabcellsep \tabcellsep \\
\tabcellsep \multicolumn{2}{l}{BMI (kg/m/m/)}\tabcellsep \multicolumn{2}{l}{Saltness test result (Normal=1, Ovservation = 2, Consultation =3)}\\
\tabcellsep Less than 25.0\tabcellsep 2.50 or more\tabcellsep BMI Less than 25.0\tabcellsep BMI 2.50 or more\\
Average±Standard deviaton\tabcellsep 27.548±2.143\tabcellsep 32.993±2.439\tabcellsep 1.578±0.665\tabcellsep 1.439±0.624\\
? test\tabcellsep \multicolumn{2}{l}{P=0.0001**}\tabcellsep \multicolumn{2}{l}{P=0.246}\\
Unpaired student-ï½?" test\tabcellsep \tabcellsep \tabcellsep \multicolumn{2}{l}{P=0.110}\\
Mann-Whaitny test\tabcellsep \multicolumn{2}{l}{P=0.0001**}\tabcellsep \tabcellsep \\
* P<0.05, ** P<0.01\tabcellsep \tabcellsep \tabcellsep \tabcellsep \end{longtable} \par
 
\caption{\label{tab_5}Table 6 :}\end{figure}
 \begin{figure}[htbp]
\noindent\textbf{7} \par 
\begin{longtable}{P{0.24174311926605505\textwidth}P{0.15336391437308866\textwidth}P{0.062385321100917435\textwidth}P{0.29892966360856266\textwidth}P{0.09357798165137615\textwidth}}
\tabcellsep \multicolumn{2}{l}{Body fat (\%)}\tabcellsep \multicolumn{2}{l}{Saltness test result (Normal=1, Ovservation = 2, Consultation =3)}\\
\tabcellsep Less than 25.0\tabcellsep 2.50 or more\tabcellsep Body fat \% Less than 25.0\tabcellsep Body fatt \% 2.50 or more\\
Average±Standard deviaton\tabcellsep 27.548±2.143\tabcellsep 32.993±2.439\tabcellsep 1.578±0.665\tabcellsep 1.388±0.593\\
? test\tabcellsep \multicolumn{2}{l}{P=0.0001**}\tabcellsep P=0.097\tabcellsep \\
Unpaired student-ï½?" test\tabcellsep \tabcellsep \tabcellsep P=017*\tabcellsep \\
Mann-Whaitny test\tabcellsep \multicolumn{2}{l}{P=0.0001**}\tabcellsep \tabcellsep \\
* P<0.05, ** P<0.01\tabcellsep \tabcellsep \tabcellsep \tabcellsep \end{longtable} \par
 
\caption{\label{tab_6}Table 7 :}\end{figure}
 \begin{figure}[htbp]
\noindent\textbf{8} \par 
\begin{longtable}{}
\end{longtable} \par
 
\caption{\label{tab_7}Table 8 :}\end{figure}
 \begin{figure}[htbp]
\noindent\textbf{9} \par 
\begin{longtable}{}
\end{longtable} \par
 
\caption{\label{tab_8}Table 9 and}\end{figure}
 \begin{figure}[htbp]
\noindent\textbf{9} \par 
\begin{longtable}{P{0.252555910543131\textwidth}P{0.15207667731629393\textwidth}P{0.06517571884984025\textwidth}P{0.30143769968051115\textwidth}P{0.07875399361022364\textwidth}}
\tabcellsep Waist (cm)\tabcellsep \tabcellsep \multicolumn{2}{l}{Salttness test result (Normal=1, Ovservation = 2, Consultation =3)}\\
\tabcellsep Less than 90.0\tabcellsep 90.0 or more\tabcellsep Waist Less than 90.0\tabcellsep Waist 90.0 or more\\
Average±Standard deviaton\tabcellsep 75.729±7.477\tabcellsep 93.733±4.338\tabcellsep 1.346±0.575\tabcellsep 1.250±0.452\\
? test\tabcellsep \multicolumn{2}{l}{P=0.0019**}\tabcellsep P=0.175\tabcellsep \\
Unpaired student-ï½?" test\tabcellsep \tabcellsep \tabcellsep P=0.452\tabcellsep \\
Mann-Whaitny test\tabcellsep \multicolumn{2}{l}{P=0.0001**}\tabcellsep \tabcellsep \\
* P<0.05, ** P<0.01\tabcellsep \tabcellsep \tabcellsep \tabcellsep \end{longtable} \par
 
\caption{\label{tab_10}Table 9 :}\end{figure}
 \begin{figure}[htbp]
\noindent\textbf{10} \par 
\begin{longtable}{}
\end{longtable} \par
 
\caption{\label{tab_11}Table 10 :}\end{figure}
 		 		\backmatter   			 
\subsection[{Acknowledgements}]{Acknowledgements}\par
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\end{document}
