# I. Introduction aintaining the integrity of natural dentition is important for the proper function and aesthetics of an individual. Endodontic therapy can play a vital role in achieving this goal. Occasionally mishaps occur during endodontic treatment, one of them is perforation of the root canal wall and furcation, which can significantly impact the long term survival of the tooth. Perforations can be defined as the mechanical or pathological communications between the root canal system and the external tooth surface. These perforations can be repaired nonsurgically with suitable biocompatible, nontoxic, radiopaque and nonabsorbent material, thus preventing bacterial contamination. In permanent teeth, several materials have been suggested for perforation repairs such as amalgam, calcium hydroxide, reinforced zinc oxide-eugenol cement, mineral trioxide aggregate (MTA), calciumenriched mixture (CEM) cement, and Biodentine. Author ?: e-mail: vidhyachandran0@gmail.com MTA is the commonly used material with a wide range of qualities. Since its introduction by Mahmoud Torabinejad in 1992, it gained an important role and emerged as a widely accepted material for various purposes. MTA was introduced by Lee et al. in 1993 for the repair of lateral root perforations. [2] It consists of dicalcium silicate, tricalcium silicate, tricalcium aluminate, and tetra calcium aluminoferrite. Although MTA has certain drawbacks such as long setting time, difficulty in manipulation, relatively high price, it has a superior sealing ability compared to other restorative materials when used for repairing perforations. In 2011 M/S Septodont introduced their new tricalcium silicate based restorative cement under the name, biodentine. It is mainly composed of highly pure tricalcium silicate, which regulates the setting reaction, calcium carbonate (filler), zirconium dioxide (radiopacifier), calcium chloride (setting accelerator), water reducing agent (superplasticizer), and water. It has been claimed that this material can be used for pulp capping, pulpotomy, apexification, root perforation, internal and external resorption, and also as a root-end filling material in periapical surgery. It is easy to handle, has a short setting time (12 minutes), has high alkaline Ph(12) and is a biocompatible material. These properties make it a favorable material for repair of perforation. # II. Materials and Method A randomized controlled in vitro trial was planned, and the study was conducted in the Department of Pedodontics and preventive dentistry, Govt Dental College, Kozhikode, Kerala in collaboration with the College of Pharmaceutical Sciences, Govt Medical College, Kozhikode. Five hundred twenty permanent molars were divided into two groups each having two hundred sixty teeth each. Molars were amputated 3mm below the furcation area. Endodontic access cavity was prepared. Orifices of the canals were negotiated, and the temporary filling was placed over the orifices of each canal. Perforation was created between the orifices to the furcation area by using a high-speed long shank round bur No:4. The tooth was completely covered, including cavity walls and pulpal floor by two successive layers of nail varnish except the area 1mm around the margin of the perforation. Group 1: MTA was mixed according to the manufacturer's instruction and carried to the perforation site with the help of MTA gun and adapted to the perforation defect with the help of hand pluggers. Moist cotton pellet was placed over MTA to allow its setting for 24 hours. Group 2: Biodentine was mixed according to the manufacturer's instruction and carried to the perforation site with the help of an amalgam carrier and adapted to the perforation defect with the help of a plugger. After sealing of the defect and setting of the material, all specimens were kept in 100% humidity for 24 hours. 2% methylene blue dye was applied inside the access cavity of all 24 hours to check the microleakage. Teeth were placed under running tap water for 30 minutes to remove all residual dye # III. Result Five hundred twenty teeth were selected. Of which 260 teeth were with MTA and the remaining with The absorbance value of 10 teeth were recorded at a time. 26 such such samples were evaluated. The data obtained were tabulated and computed statistically using independent T-test. Mean and standard deviation were estimated for each group to evaluate the absorbance of MTA and biodentine. Samples (10 teeth)) were then placed in vials containing 65% nitric acid for 3 days for the extraction of the dye. The vials were centrifuged at 14000rpm for 5minutes to separate debris from the extracted dye. About 2ml of the supernatant was then analyzed in a UV visible spectrophotometer at 550nm wavelength using concentrated nitric acid as the blank, and readings were recorded as absorbance units. # Graph 1: Comparison of mean absorbance values of MTA and Biodentine # IV. Discussion The success of the furcation repair depends on various factors like size and location of the defect, time and duration of exposure to contamination, the material used for repair, the possibility of sealing the perforation, etc. A factor that is under the control of the operator is the choice of material to be used that enhances treatment outcome. To obtain success, the perforation repair material should ideally trigger the formation of new bone, periodontal ligament and cementum. In the search of the ideal material, numerous sealing materials and techniques have been tested over the years with varying success. The search for alternative agents has been aimed to overcome the drawbacks of previously used materials to reduce the cost and to increase the feasibility of both professionals and patients. The present study is the first of its kind to compare the sealing ability of Biodentine and MTA in repairing the furcal perforation in permanent molars using the dye penetration technique. There are several methods that can be employed to evaluate the sealing ability of repair materials other than dye penetration technique, like SEM, fluid filtration technique, bacterial and protein leakage model, etc. Recent methods include radioactive isotopes, artificial caries, neutron activation analysis and electrical conductivity. The present study has utilized methylene blue as a dye because it is inexpensive, easy to use, has a high degree of staining and a molecular weight even lower than that of bacterial toxins. With regard to dyes particle size, pH and chemical reactivity are believed to affect the degree of penetration. Biodentine is very similar to MTA in basic composition. The manufacturers claim that the addition of setting accelerators and softeners, in a new predosed capsule formulation for use in a mixing device predominantly improves the physical properties of the material, making it more user-friendly. Biodentine does not require two-step obturation as the setting is faster and thus has a lower risk of bacterial contamination making it superior to MTA. Allwyn Samuel et al (2016) evaluated the sealing ability of biodentine and MTA in primary molars using SEM and concluded that biodentine showed significantly less leakage (0.149) compared to MTA(0,583). Soundappan et al (2014) comparison with MTA and intermediate restorative material (IRM) using SEM. They conducted the study using thirty permanent central incisors and stated that in overall comparison, MTA and IRM were significantly superior when compared to Biodentine in terms of marginal adaptation when used as retrograde filling material. El Choudary HM et al (2011) evaluated sealing ability of four calcium containing cements (MTA, Portland cement, biodentine and tech biosealer). The study showed there was no significant difference between the mean microleakage values obtained in the four tested materials after 24 hours, 1 month, 6 months and 1year. But the microleakage values for each individual material were significantly higher at 24 hours than at the other time intervals. # V. Conclusion Artificially made furcal perforations in permanent molars were randomly sealed with MTA and Biodentine. The teeth were then evaluated for methylene blue dye leakage. The dye extracted using nitric acid was assessed in a UV spectrophotometer and the absorbance units were recorded. In this study, MTA showed higher microleakage and hence higher absorbance values than Biodentine. From the above inferences, the study concluded that Biodentine with better sealing ability compared to MTA and may be a good alternative to MTA in the management of furcal perforations in permanents molars, thereby increasing the longevity of the tooth. 1GROUPMATERIAL USEDNMEAN ABSORBANCE1MTA260.05562BIODENTIN260.0046 2YearVolume XX Issue VII Version ID D D D )(Medical ResearchGlobal Journal ofGROUPMATERIAL USEDNSTANDARD DEVIATIONP VALUE1MTA260.05560.0012BIODENTINE260.00460.0012020J © 2020 Global Journals Financial support and sponsorship: Nil. ## Conflicts of interest: There are no conflicts of interest. * Microleakage of CEM cement and ProRoot MTA as furcal perforation repair materials in primary teeth RHaghgoo SArfa SAsgary Iran Endod J 8 2013 PMC free article. 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