\documentclass[11pt,twoside]{article}\makeatletter

\IfFileExists{xcolor.sty}%
  {\RequirePackage{xcolor}}%
  {\RequirePackage{color}}
\usepackage{colortbl}
\usepackage{wrapfig}
\usepackage{ifxetex}
\ifxetex
  \usepackage{fontspec}
  \usepackage{xunicode}
  \catcode`⃥=\active \def⃥{\textbackslash}
  \catcode`❴=\active \def❴{\{}
  \catcode`❵=\active \def❵{\}}
  \def\textJapanese{\fontspec{Noto Sans CJK JP}}
  \def\textChinese{\fontspec{Noto Sans CJK SC}}
  \def\textKorean{\fontspec{Noto Sans CJK KR}}
  \setmonofont{DejaVu Sans Mono}
  
\else
  \IfFileExists{utf8x.def}%
   {\usepackage[utf8x]{inputenc}
      \PrerenderUnicode{–}
    }%
   {\usepackage[utf8]{inputenc}}
  \usepackage[english]{babel}
  \usepackage[T1]{fontenc}
  \usepackage{float}
  \usepackage[]{ucs}
  \uc@dclc{8421}{default}{\textbackslash }
  \uc@dclc{10100}{default}{\{}
  \uc@dclc{10101}{default}{\}}
  \uc@dclc{8491}{default}{\AA{}}
  \uc@dclc{8239}{default}{\,}
  \uc@dclc{20154}{default}{ }
  \uc@dclc{10148}{default}{>}
  \def\textschwa{\rotatebox{-90}{e}}
  \def\textJapanese{}
  \def\textChinese{}
  \IfFileExists{tipa.sty}{\usepackage{tipa}}{}
\fi
\def\exampleFont{\ttfamily\small}
\DeclareTextSymbol{\textpi}{OML}{25}
\usepackage{relsize}
\RequirePackage{array}
\def\@testpach{\@chclass
 \ifnum \@lastchclass=6 \@ne \@chnum \@ne \else
  \ifnum \@lastchclass=7 5 \else
   \ifnum \@lastchclass=8 \tw@ \else
    \ifnum \@lastchclass=9 \thr@@
   \else \z@
   \ifnum \@lastchclass = 10 \else
   \edef\@nextchar{\expandafter\string\@nextchar}%
   \@chnum
   \if \@nextchar c\z@ \else
    \if \@nextchar l\@ne \else
     \if \@nextchar r\tw@ \else
   \z@ \@chclass
   \if\@nextchar |\@ne \else
    \if \@nextchar !6 \else
     \if \@nextchar @7 \else
      \if \@nextchar (8 \else
       \if \@nextchar )9 \else
  10
  \@chnum
  \if \@nextchar m\thr@@\else
   \if \@nextchar p4 \else
    \if \@nextchar b5 \else
   \z@ \@chclass \z@ \@preamerr \z@ \fi \fi \fi \fi
   \fi \fi  \fi  \fi  \fi  \fi  \fi \fi \fi \fi \fi \fi}
\gdef\arraybackslash{\let\\=\@arraycr}
\def\@textsubscript#1{{\m@th\ensuremath{_{\mbox{\fontsize\sf@size\z@#1}}}}}
\def\Panel#1#2#3#4{\multicolumn{#3}{){\columncolor{#2}}#4}{#1}}
\def\abbr{}
\def\corr{}
\def\expan{}
\def\gap{}
\def\orig{}
\def\reg{}
\def\ref{}
\def\sic{}
\def\persName{}\def\name{}
\def\placeName{}
\def\orgName{}
\def\textcal#1{{\fontspec{Lucida Calligraphy}#1}}
\def\textgothic#1{{\fontspec{Lucida Blackletter}#1}}
\def\textlarge#1{{\large #1}}
\def\textoverbar#1{\ensuremath{\overline{#1}}}
\def\textquoted#1{‘#1’}
\def\textsmall#1{{\small #1}}
\def\textsubscript#1{\@textsubscript{\selectfont#1}}
\def\textxi{\ensuremath{\xi}}
\def\titlem{\itshape}
\newenvironment{biblfree}{}{\ifvmode\par\fi }
\newenvironment{bibl}{}{}
\newenvironment{byline}{\vskip6pt\itshape\fontsize{16pt}{18pt}\selectfont}{\par }
\newenvironment{citbibl}{}{\ifvmode\par\fi }
\newenvironment{docAuthor}{\ifvmode\vskip4pt\fontsize{16pt}{18pt}\selectfont\fi\itshape}{\ifvmode\par\fi }
\newenvironment{docDate}{}{\ifvmode\par\fi }
\newenvironment{docImprint}{\vskip 6pt}{\ifvmode\par\fi }
\newenvironment{docTitle}{\vskip6pt\bfseries\fontsize{22pt}{25pt}\selectfont}{\par }
\newenvironment{msHead}{\vskip 6pt}{\par}
\newenvironment{msItem}{\vskip 6pt}{\par}
\newenvironment{rubric}{}{}
\newenvironment{titlePart}{}{\par }

\newcolumntype{L}[1]{){\raggedright\arraybackslash}p{#1}}
\newcolumntype{C}[1]{){\centering\arraybackslash}p{#1}}
\newcolumntype{R}[1]{){\raggedleft\arraybackslash}p{#1}}
\newcolumntype{P}[1]{){\arraybackslash}p{#1}}
\newcolumntype{B}[1]{){\arraybackslash}b{#1}}
\newcolumntype{M}[1]{){\arraybackslash}m{#1}}
\definecolor{label}{gray}{0.75}
\def\unusedattribute#1{\sout{\textcolor{label}{#1}}}
\DeclareRobustCommand*{\xref}{\hyper@normalise\xref@}
\def\xref@#1#2{\hyper@linkurl{#2}{#1}}
\begingroup
\catcode`\_=\active
\gdef_#1{\ensuremath{\sb{\mathrm{#1}}}}
\endgroup
\mathcode`\_=\string"8000
\catcode`\_=12\relax

\usepackage[a4paper,twoside,lmargin=1in,rmargin=1in,tmargin=1in,bmargin=1in,marginparwidth=0.75in]{geometry}
\usepackage{framed}

\definecolor{shadecolor}{gray}{0.95}
\usepackage{longtable}
\usepackage[normalem]{ulem}
\usepackage{fancyvrb}
\usepackage{fancyhdr}
\usepackage{graphicx}
\usepackage{marginnote}

\renewcommand{\@cite}[1]{#1}


\renewcommand*{\marginfont}{\itshape\footnotesize}

\def\Gin@extensions{.pdf,.png,.jpg,.mps,.tif}

  \pagestyle{fancy}

\usepackage[pdftitle={Seroprevalence of HIV, HBV and HCV Infectivity among Blood Donors in Sudan},
 pdfauthor={}]{hyperref}
\hyperbaseurl{}

	 \paperwidth210mm
	 \paperheight297mm
              
\def\@pnumwidth{1.55em}
\def\@tocrmarg {2.55em}
\def\@dotsep{4.5}
\setcounter{tocdepth}{3}
\clubpenalty=8000
\emergencystretch 3em
\hbadness=4000
\hyphenpenalty=400
\pretolerance=750
\tolerance=2000
\vbadness=4000
\widowpenalty=10000

\renewcommand\section{\@startsection {section}{1}{\z@}%
     {-1.75ex \@plus -0.5ex \@minus -.2ex}%
     {0.5ex \@plus .2ex}%
     {\reset@font\Large\bfseries}}
\renewcommand\subsection{\@startsection{subsection}{2}{\z@}%
     {-1.75ex\@plus -0.5ex \@minus- .2ex}%
     {0.5ex \@plus .2ex}%
     {\reset@font\Large}}
\renewcommand\subsubsection{\@startsection{subsubsection}{3}{\z@}%
     {-1.5ex\@plus -0.35ex \@minus -.2ex}%
     {0.5ex \@plus .2ex}%
     {\reset@font\large}}
\renewcommand\paragraph{\@startsection{paragraph}{4}{\z@}%
     {-1ex \@plus-0.35ex \@minus -0.2ex}%
     {0.5ex \@plus .2ex}%
     {\reset@font\normalsize}}
\renewcommand\subparagraph{\@startsection{subparagraph}{5}{\parindent}%
     {1.5ex \@plus1ex \@minus .2ex}%
     {-1em}%
     {\reset@font\normalsize\bfseries}}


\def\l@section#1#2{\addpenalty{\@secpenalty} \addvspace{1.0em plus 1pt}
 \@tempdima 1.5em \begingroup
 \parindent \z@ \rightskip \@pnumwidth 
 \parfillskip -\@pnumwidth 
 \bfseries \leavevmode #1\hfil \hbox to\@pnumwidth{\hss #2}\par
 \endgroup}
\def\l@subsection{\@dottedtocline{2}{1.5em}{2.3em}}
\def\l@subsubsection{\@dottedtocline{3}{3.8em}{3.2em}}
\def\l@paragraph{\@dottedtocline{4}{7.0em}{4.1em}}
\def\l@subparagraph{\@dottedtocline{5}{10em}{5em}}
\@ifundefined{c@section}{\newcounter{section}}{}
\@ifundefined{c@chapter}{\newcounter{chapter}}{}
\newif\if@mainmatter 
\@mainmattertrue
\def\chaptername{Chapter}
\def\frontmatter{%
  \pagenumbering{roman}
  \def\thechapter{\@roman\c@chapter}
  \def\theHchapter{\roman{chapter}}
  \def\thesection{\@roman\c@section}
  \def\theHsection{\roman{section}}
  \def\@chapapp{}%
}
\def\mainmatter{%
  \cleardoublepage
  \def\thechapter{\@arabic\c@chapter}
  \setcounter{chapter}{0}
  \setcounter{section}{0}
  \pagenumbering{arabic}
  \setcounter{secnumdepth}{6}
  \def\@chapapp{\chaptername}%
  \def\theHchapter{\arabic{chapter}}
  \def\thesection{\@arabic\c@section}
  \def\theHsection{\arabic{section}}
}
\def\backmatter{%
  \cleardoublepage
  \setcounter{chapter}{0}
  \setcounter{section}{0}
  \setcounter{secnumdepth}{2}
  \def\@chapapp{\appendixname}%
  \def\thechapter{\@Alph\c@chapter}
  \def\theHchapter{\Alph{chapter}}
  \appendix
}
\newenvironment{bibitemlist}[1]{%
   \list{\@biblabel{\@arabic\c@enumiv}}%
       {\settowidth\labelwidth{\@biblabel{#1}}%
        \leftmargin\labelwidth
        \advance\leftmargin\labelsep
        \@openbib@code
        \usecounter{enumiv}%
        \let\p@enumiv\@empty
        \renewcommand\theenumiv{\@arabic\c@enumiv}%
	}%
  \sloppy
  \clubpenalty4000
  \@clubpenalty \clubpenalty
  \widowpenalty4000%
  \sfcode`\.\@m}%
  {\def\@noitemerr
    {\@latex@warning{Empty `bibitemlist' environment}}%
    \endlist}

\def\tableofcontents{\section*{\contentsname}\@starttoc{toc}}
\parskip0pt
\parindent1em
\def\Panel#1#2#3#4{\multicolumn{#3}{){\columncolor{#2}}#4}{#1}}
\newenvironment{reflist}{%
  \begin{raggedright}\begin{list}{}
  {%
   \setlength{\topsep}{0pt}%
   \setlength{\rightmargin}{0.25in}%
   \setlength{\itemsep}{0pt}%
   \setlength{\itemindent}{0pt}%
   \setlength{\parskip}{0pt}%
   \setlength{\parsep}{2pt}%
   \def\makelabel##1{\itshape ##1}}%
  }
  {\end{list}\end{raggedright}}
\newenvironment{sansreflist}{%
  \begin{raggedright}\begin{list}{}
  {%
   \setlength{\topsep}{0pt}%
   \setlength{\rightmargin}{0.25in}%
   \setlength{\itemindent}{0pt}%
   \setlength{\parskip}{0pt}%
   \setlength{\itemsep}{0pt}%
   \setlength{\parsep}{2pt}%
   \def\makelabel##1{\upshape ##1}}%
  }
  {\end{list}\end{raggedright}}
\newenvironment{specHead}[2]%
 {\vspace{20pt}\hrule\vspace{10pt}%
  \phantomsection\label{#1}\markright{#2}%

  \pdfbookmark[2]{#2}{#1}%
  \hspace{-0.75in}{\bfseries\fontsize{16pt}{18pt}\selectfont#2}%
  }{}
      \def\TheFullDate{2022 1970-01-01 (revised: 35 Year 2022 01 January 1970)}
\def\TheID{\makeatother }
\def\TheDate{2022 1970-01-01}
\title{Seroprevalence of HIV, HBV and HCV Infectivity among Blood Donors in Sudan}
\author{}\makeatletter 
\makeatletter
\newcommand*{\cleartoleftpage}{%
  \clearpage
    \if@twoside
    \ifodd\c@page
      \hbox{}\newpage
      \if@twocolumn
        \hbox{}\newpage
      \fi
    \fi
  \fi
}
\makeatother
\makeatletter
\thispagestyle{empty}
\markright{\@title}\markboth{\@title}{\@author}
\renewcommand\small{\@setfontsize\small{9pt}{11pt}\abovedisplayskip 8.5\p@ plus3\p@ minus4\p@
\belowdisplayskip \abovedisplayskip
\abovedisplayshortskip \z@ plus2\p@
\belowdisplayshortskip 4\p@ plus2\p@ minus2\p@
\def\@listi{\leftmargin\leftmargini
               \topsep 2\p@ plus1\p@ minus1\p@
               \parsep 2\p@ plus\p@ minus\p@
               \itemsep 1pt}
}
\makeatother
\fvset{frame=single,numberblanklines=false,xleftmargin=5mm,xrightmargin=5mm}
\fancyhf{} 
\setlength{\headheight}{14pt}
\fancyhead[LE]{\bfseries\leftmark} 
\fancyhead[RO]{\bfseries\rightmark} 
\fancyfoot[RO]{}
\fancyfoot[CO]{\thepage}
\fancyfoot[LO]{\TheID}
\fancyfoot[LE]{}
\fancyfoot[CE]{\thepage}
\fancyfoot[RE]{\TheID}
\hypersetup{citebordercolor=0.75 0.75 0.75,linkbordercolor=0.75 0.75 0.75,urlbordercolor=0.75 0.75 0.75,bookmarksnumbered=true}
\fancypagestyle{plain}{\fancyhead{}\renewcommand{\headrulewidth}{0pt}}

\date{}
\usepackage{authblk}

\providecommand{\keywords}[1]
{
\footnotesize
  \textbf{\textit{Index terms---}} #1
}

\usepackage{graphicx,xcolor}
\definecolor{GJBlue}{HTML}{273B81}
\definecolor{GJLightBlue}{HTML}{0A9DD9}
\definecolor{GJMediumGrey}{HTML}{6D6E70}
\definecolor{GJLightGrey}{HTML}{929497} 

\renewenvironment{abstract}{%
   \setlength{\parindent}{0pt}\raggedright
   \textcolor{GJMediumGrey}{\rule{\textwidth}{2pt}}
   \vskip16pt
   \textcolor{GJBlue}{\large\bfseries\abstractname\space}
}{%   
   \vskip8pt
   \textcolor{GJMediumGrey}{\rule{\textwidth}{2pt}}
   \vskip16pt
}

\usepackage[absolute,overlay]{textpos}

\makeatother 
      \usepackage{lineno}
      \linenumbers
      
\begin{document}

             \author[1]{Amged  Hussein}

\renewcommand\Authands{ and }

\date{\small \em Received: 1 January 1970 Accepted: 1 January 1970 Published: 1 January 1970}

\maketitle


\begin{abstract}
        


Numerous infectious diseases are spread by blood transfusion, particularly viral infections. The hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus (HIV), and other pathogenic organisms are transmitted through inappropriate screening of blood products (Nilima Sawke, et al., 2013).These infected blood products are causing fatal, persistent and life frightening disorders (WHO, 2012). Aim of the current study was to estimate a statistical of the incidence of HBV, HCV, and HIV among blood donors in Sudan. Results:In the blood supplies system in Sudan the total average of voluntary blood donors (VBD) was10.1%, whereas the family replacement donation (FBD) was 89.9% (3), the total incidence in blood donors was 3.2%.

\end{abstract}


\keywords{human immunodeficiency virus, , seroprevalence, blood donors.}

\begin{textblock*}{18cm}(1cm,1cm) % {block width} (coords) 
\textcolor{GJBlue}{\LARGE Global Journals \LaTeX\ JournalKaleidoscope\texttrademark}
\end{textblock*}

\begin{textblock*}{18cm}(1.4cm,1.5cm) % {block width} (coords) 
\textcolor{GJBlue}{\footnotesize \\ Artificial Intelligence formulated this projection for compatibility purposes from the original article published at Global Journals. However, this technology is currently in beta. \emph{Therefore, kindly ignore odd layouts, missed formulae, text, tables, or figures.}}
\end{textblock*}


\let\tabcellsep& 	 	 		 
\section[{Introduction}]{Introduction}\par
lood transfusion transfers of blood and its components such as red blood cells, platelets, and plasma from donor to the recipient \hyperref[b12]{(WHO, 2011)}. The donation of the blood saves the lives of millions of people universally, and it is essential to the helpfulness of the health system by supporting current transfusions worldwide (WHO, GDB, 2011).The following tests were mandatory performed in Sudan, at all blood centers at all levels following WHO, international organizations and regulatory bodies for blood safety: Hepatitis B surface antigen (HBsAg), anti-HIV1, HIV2, and an approved test for anti-HCV. All three tests have to be negative. \hyperref[b3]{(Roger Y. Dodd, 2001)}. All reactive results of blood donor's samples for infectious transmitted disease were should be retested in duplicate by the same assay. (WHO, 2012). All confirmed contaminated blood components units by TTI through repeatedly testing samples were not used for therapeutic applications and should normally be destroyed unless useful for non-therapeutic purposes or investigations. All blood donors have reactive testing( D D D D )\par
F results should be evaluated by a confirmatory tests and there should be a mechanism to inform blood donors the positive testing results. (WHO, Geneva, 2013). It is recommended that national testing algorithms for TTI shall be developed and used to enable consistent resolution of discordant indeterminate or unconfirmed results. (Jain C,. et al, 2011). In some African countries, in addition to TTI markers other serological tests were performed, for instance, anti-HBC testing may be performed on whole blood donations to further reduce the risk of exposure of recipients to HBV by contaminated blood or blood components to supply of safe blood products for transfusion, it's compulsory to introduce an advanced technology like a nucleic acid test (NAT) because of excellent clinical sensitivity and good specificity to detect infected blood components as it identified pathogens prior in the 'window period' than enzymes immune assay \hyperref[b22]{(Gerard C,, . et al , 1995)}. Even though, it has some margin in blood components with a lesser range of viremia, which can even free quantifiable by NAT (WHO, Geneva, 2012). Even with this margin, the grouping of both enzymes immune assay and NAT has notably condensed the hazard of pathogen spread during transfusion \hyperref[b9]{(11)}. Also, many scientific research data showed that the comparison between p24 antigen detection or conventional serological testing, it is estimated that the use of NAT reduces the detection time from 22 to 11 days for HIV; from 70 to 10 days for HCV, and from 60 to 30 days for HBV infection (H. \hyperref[b4]{Sheikholeslami,. et al, 2010)}. Additional testing for other agents or markers such as anti-HTLV I, II, anti-T.cruzi, or West Nile virus (WNV) may be taking into account the epidemiological situation in any given region or country or the frequency of donating blood (H.W.  {\ref Reesink, 2000)}. In addition to testing TTI markers serologically, Nucleic Acid Testing (NAT) testing of blood donations for the virus genomes has been introduced in some countries to increase the chance of identifying infected blood donors. Testing for the presence of nucleic acid may be performed for viruses such as HCV, HBV, HIV, HTLV, and WNV and or Parvovirus B19, and the application of this technology may be extended to other transmissible microbes (M.M.  {\ref E Nageh,. et al, 1994)}. Nucleic Acid Testing (NAT) require a sophisticated laboratory environment, special equipment, and specially trained laboratory personnel. To supply of safe blood products for transfusion, it's compulsory to introduce an advanced technology like a nucleic acid test (NAT) because of excellent clinical sensitivity and good specificity to detect infected blood components as it identified pathogens prior in the 'window period' than enzymes immune assay to supply of safe blood product for transfusion, it's compulsory to introduce an advanced technology like a nucleic acid test (NAT) because of excellent clinical sensitivity and good specificity to detect infected blood components as it identified pathogens prior in the 'window period' than enzymes immune assay (WHO, GDB,2011).Even though, it has some margin in blood components with a lesser range of viremia, which can even free quantifiable by NAT. Even with this margin, the grouping of both enzymes immune assay and NAT has notably condensed the hazard of pathogen spread during transfusion (WHO, Geneva, 2002).Even though, it has some margin in blood components with a lesser range of viremia, which can even free quantifiable by NAT(WHO, Geneva,2012). Even with this margin, the grouping of both enzymes immune assay and NAT reduced the hazard of pathogen spread through blood \hyperref[b21]{(Widman FK, 1985)}. Mainly because of an extraordinary risk of false-positive testing results due to contamination when NATs were performed to donor samples, therefore very stringent handling and logistics are mandatory.  {\ref (WHO, 2008} {\ref (WHO, -2015))}. In contrast to testing of individual blood donor specimen's serologically for TTI markers, NAT testing can be performed following assembling various samples in mini-pools . (WHO, Geneva , 2014 ). However, this requires thoroughly validated laboratory systems including samples labeling, a validated strategy and pooling process, a validated algorithm to resolve pool results to individual donors. Hence, specific logistics systems shall be established at all laboratory and blood transfusion services process to collect suitably label samples. (WHO, Geneva, 2011). Contiguously tracing blood samples through the whole process from blood donation, through pooling samples, testing, and release of the testing results may present a particularly demanding challenge. A system should exist in the country or region for approval of laboratory testing systems, such as accredited laboratory or council. (WHO.  {\ref GDB, 1998} {\ref GDB, -1999))}.The blood transfusion department contains clinical methods and guidelines for blood screening before transfusion. If the screening procedure and other regulations are not followed well, there is a possibility to carry the risk of spreading blood transfusion contagious pathogens like HIV, HBV, HCV, Bacteria (syphilis), and others (WHO Geneva, 2012). Also, there is a 1\% of chance of transfusion -related infection in each unit of blood even if the procedure is followed well  {\ref (WHO, 2002)} .Therefore, the risk of blood transfusion -transmitted infection today is minimized than constantly, the delivery of safe blood products stays behind inquiry to infection with accepted and until now to be predictable human pathogens (WHO, June 2011). 
\section[{II.}]{II.} 
\section[{Main Text}]{Main Text}\par
In the present study were incorporated 500 blood donors. All the donors have been screened with a medical consultant before donation, who attended as voluntary and replacement in blood transfusion centers in all states from January 2014 to December 2015. 
\section[{III.}]{III.} 
\section[{Sample Collection}]{Sample Collection}\par
Five milliliters (5ml) of venous blood were collected from each donor after taking history and clinical examination using plain vacationer tubes during donated blood. All samples were allowed to clot formation and then were centrifuged at 3000 rpm for 10 minutes. All serum samples were separated into sterile 2ml cry vial containers and stored at -20°C until used. All serum samples were shipped and transported from the states to the national blood directorate in the Khartoum within the acceptable period, and temperature using cool boxes containing ice bags, temperature -controlled in each cool box using thermostats.\par
IV. 
\section[{Serology}]{Serology}\par
All donors samples were screened by ELISA kits from fortress diagnostic Unit 2C Antrim technology park, Antrim BT41 IQS (United Kingdom): the least most negligible (cut off) was considered as per company guidelines for reporting positive and negative outcomes. Actual positive and negative samples were used subjectively as an outside run in each screening for our laboratory intention. The donated blood was discarded if the serum sample was positive for any infectivity. The statistical analysis was done using Microsoft ware office excel 2007.\par
V. 
\section[{Results}]{Results}\par
Two hundred samples were collected from blood donors for TTI markers (HIV, HBV, and HCV) testing. One hundred samples were collected from family replacement blood donors and another one hundred samples were collected from voluntary blood donors. All models were tested for HIV, HBV, and HCV using the ELISA technique. 4 models have positive results for HBV and one model had positive effects for HCV from the FBD group, in contrast all models from VBD were negative for TTI markers as presented in fig  
\section[{Discussion}]{Discussion}\par
Overall average laboratory testing principle in the blood transfusion services in Sudan was found to be 54.18 \% The world health organization (WHO) recommends that all donated blood be screened for HIV1\&11, HBV, HCV, and syphilis (WHO, 2009) .The lowest incidence and prevalence of transfusiontransmissible infections is generally found among regular voluntary non-remunerated donors rather than first-time or occasional donors and family replacement blood donors (WHO,  {\ref GDB reports, 2001} {\ref GDB reports, -2002))}.The tested results of samples from family replacement blood donors (FRBD) show that there are 5.6\% seropositive for HBV was found in family replacement blood donors while in contrast the number of representatives from voluntary blood donors (VBD) was found free of viral transfusion transmissible inactions such as HIV, HBV and HCV. The obtained results by this study was in high agreement With results done in the African countries, and the results shows that the prevalence of hepatitis B among blood donors in WHO African Region countries were 5-15\% and the prevalence of hepatitis C among blood donors in Cameroon 8.8\% , in Tanzania and Africa 5-15\% (DR Neelam, June 2006 ).HIV causes significant health problems in sub -Saharan Africa where the prevalence of HIV among blood donors ranges between 2-20\% similarly: the prevalence of HCV was 4.8\% in Cameron,1.5\% in Tanzania (WHO, 2013). And high in Egypt 13.6 \% (Martin, H and Jeffery's, 2011). Hepatitis B prevalence was 2.1\% and Hepatitis C, 13.6\% among blood donors in Egypt \hyperref[b6]{(Egypt, 2016)}. 
\section[{VII.}]{VII.} 
\section[{Conclusion}]{Conclusion}\par
Comparison of seroprevalence of (TTI) between family replacement and voluntary blood donors shows that there are 5.6\% of family replacement blood donors has positive HBV results, which increase the risk of transfusion of infected blood in contrast all voluntary blood donors show that the testing result for (TTI) markers are negative which that the blood supply through voluntary blood donors is safest than family replacement blood donation system. The seroprevalence rate was low in voluntary blood donors compared to family replacement blood donors because regularly voluntary blood donors are safe and recommended by WHO. 
\section[{VIII.}]{VIII.} 
\section[{Limitation of the Study}]{Limitation of the Study}\par
In this study, rapid and ELISA techniques were used to diagnose the infection. Although it has a specific accuracy, it is currently used to diagnose diseases. Also no previous studies data in Sudan were used for comparison. 
\section[{List of abbreviations Not applicable}]{List of abbreviations Not applicable} 
\section[{Declarations Ethical approval and consent to participant}]{Declarations Ethical approval and consent to participant}\par
Approval of conducting this study was obtained from the National public health laboratory, Khartoum, Sudan. Written consent was taken from each member of the study.\par
Approval of this study was obtained from the National public health laboratory, Khartoum, Sudan. Written consent was taken from each member of the study. 
\section[{Consent for publication}]{Consent for publication}\par
Not applicable. \begin{figure}[htbp]
\noindent\textbf{}\includegraphics[]{image-2.png}
\caption{\label{fig_1}}\end{figure}
 			\footnote{© 2022 Global JournalsSeroprevalence of HIV, HBV and HCV Infectivity among Blood Donors in Sudan} 		 		\backmatter   			 
\subsection[{Acknowledgment}]{Acknowledgment}\par
We acknowledge the support provided by technical staff from blood banks and centers in all states. Also, to all my collages in national blood transfusion center and coagulation reference laboratorynational public health laboratory. Results of tested quality parameters the samples collected from these units. 			  			 
\subsection[{Availability of data and materials}]{Availability of data and materials}\par
The datasets generated during and/or analyzed in this study are not publicly available due to the National public health laboratory, Khartoum, Sudan, ethical policy to protect participant confidentiality. 
\subsection[{Competing interest}]{Competing interest}\par
The authors declare that they have no competing interests. 
\subsection[{Funding}]{Funding}\par
No funding was obtained for this study Authors contributions KM and AA contributed to literature search and manuscript writing. KM had the main idea of the study and contributed to manuscript writing; EW contributed to clinic work; AH contributed to statistical analysis. A supervised the study and critically reviewed the manuscript .All authors read and approved the final draft of the manuscript.			 			  				\begin{bibitemlist}{1}
\bibitem[Who ()]{b17}\label{b17} 	 		\textit{blood donor counseling, implementation guidelines in collaboration with centers for disease control and prevention},  		 			Geneva Who 		.  		2014. U.S..  	 	 () and international federation of red cross and red crescent societies) 
\bibitem[Who ()]{b26}\label{b26} 	 		\textit{Blood donor selection, guidelines on assessing donor suitability for blood donation},  		 			Geneva Who 		.  		 NLM classification: WH 460.  		2012.  	 
\bibitem[blood Safety, key global fact and figures (2011)]{b7}\label{b7} 	 		\textit{blood Safety, key global fact and figures},  		 \url{http://www.who.int/bloodsafety/globaldatabase/en}  		June 2011.  		 			WHO 		 	 
\bibitem[Nageh ()]{b0}\label{b0} 	 		\textit{Blood transfusion a basic text, world health organization regional publication},  		 			M M Nageh 		.  		1994. eastern Mediterranean, Series.  	 
\bibitem[Blood transfusion safety manual WHO ()]{b27}\label{b27} 	 		‘Blood transfusion safety manual’.  		 \url{http://www.who.int/bloodsafety/en}  	 	 		\textit{WHO}  		2012.  	 
\bibitem[Egypt ()]{b6}\label{b6} 	 		\textit{Comparison of seropositivity of HIV, HBV, HCV and Syphilis and Malaria in replacement and voluntary blood donors in Western India},  		 			Egypt 		.  		2016. 2011.  	 	 (blood transfusion in history, available from: www.ishim.net/../blood transfusion history) 
\bibitem[department of blood safety Geneva ()]{b16}\label{b16} 	 		 \url{http://www.who.int/bloodsafety/transfusionservices/en/Blood\textunderscore Safety\textunderscore Eng.pdf}  	 	 		\textit{department of blood safety Geneva},  				2002.  	 
\bibitem[Sheikholeslami ()]{b4}\label{b4} 	 		‘eastern Mediterranean health journal, transfusion audit of blood products using the world Health organization basic information sheet in Qazvin’.  		 			H Sheikholeslami 		.  	 	 		\textit{EMHJ}  		2010. 16  (12) .  		 			Islamic republic of Iran 		 	 
\bibitem[Gerard ()]{b22}\label{b22} 	 		\textit{European commission 25. WHO, Geneva, 2013, safe blood and blood products-introductory, guidelines and principles for safe blood transfusion practice},  		 			C Gerard 		.  		 \url{WHO/GPA/CNP/93.2A}  		1995.  	 	 (safe blood in developing countries, principles and organization Brussels) 
\bibitem[Republic Of Sudan ()]{b2}\label{b2} 	 		\textit{Federal Ministry of Health, directorate of national public laboratory: Annual services reports in the period of},  		 			Republic Of Sudan 		.  		2013/2014, 2015, and 2016.  	 
\bibitem[Global Database on Blood safety in summary report ()]{b19}\label{b19} 	 		\textit{Global Database on Blood safety in summary report},  		 \url{http://www.who.int/bloodsafety}  		2009.  		 			WHO 		 	 	 (blood safety indicators (GDBS) 
\bibitem[laboratory quality management system WHO ()]{b23}\label{b23} 	 		‘laboratory quality management system’.  		 \url{Availablefromwww.who.int}  	 	 		\textit{WHO}  		2011.  	 
\bibitem[Who and Neelam (2006)]{b9}\label{b9} 	 		\textit{Making safe blood available in Africa, a committee on international relations subcommittee non Africa, Global human rights and international operations U.S. -Martin Hensher and Emma Jeffery's, 2011, Financing blood transfusion services in sub-Saharan Africa: A role for user fees},  		 			Who 		,  		 			Neelam 		.  		 \url{http://www.who.int/bloodsafety/makingsafebloodavailableinAfricastatement.pdf}  		June 2006. oxford university press. 15 p. .  	 
\bibitem[Who (ed.)]{b8}\label{b8} 	 		\textit{Office for Africa 2009: status of blood safety in the WHO Africa region, report of the 2006 servey},  		 			Who 		.  		J.B.Toko, Paul Mainuka and A.J.Diaarra-Nama (ed.)  		 	 
\bibitem[Roger and Dodd ()]{b3}\label{b3} 	 		‘Overview of infectious disease in blood safety and surveillance edited by Jeanne V’.  		 			Y Roger 		,  		 			Dodd 		.  	 	 		\textit{Linde center new york copyright by marcel Dekker, Chapter},  				2001. p. .  	 	 (III, A -8) 
\bibitem[Das and Taswell]{b5}\label{b5} 	 		\textit{Rees ink, 2000, transmittable Diseases and blood transfusion in: quality assurance in blood banking and its clinical impact, editor's .Smith Sibinga Ct},  		 			HW 		,  		 			P 		,  		 			C Das 		,  		 			H F Taswell 		.  		 	 
\bibitem[Who ()]{b15}\label{b15} 	 		\textit{safe blood and blood products-introductory (Guidelines and principles for safe blood transfusion practice)},  		 			Geneva Who 		.  		 \url{WHO/GPA/CNP/93.2A}  		2012.  	 
\bibitem[Who ()]{b12}\label{b12} 	 		\textit{safe blood and blood products-module 2, screening for HIV and other infectious agent, WHO/G86},  		 			Geneva Who 		.  		 \url{http://www.who.int/bloodsafety}  		2011.  	 
\bibitem[Sawke ()]{b1}\label{b1} 	 		\textit{Seroprevalence of common transfusion-transmitted infections among blood donors, people's journal of scientific research},  		 			Nilima Sawke 		.  		2013.  	 
\bibitem[Widman ()]{b21}\label{b21} 	 		\textit{Technical manual, American association of blood banks},  		 			F K Widman 		.  		1985. Arlington. p. .  	 
\bibitem[the clinical use of blood hand book. blood transfusion safety World Health Organization (2002)]{b10}\label{b10} 	 		‘the clinical use of blood hand book. blood transfusion safety’.  		 \url{www.who.int/bloodsafety/clinicaluse/en/Handbook\textunderscore EN.pdf}  	 	 		\textit{World Health Organization}  		2002. August 2009.  	 
\bibitem[Universal access to safe blood WHO ()]{b20}\label{b20} 	 		‘Universal access to safe blood’.  		 \url{www.WHO.int/bloodsafety}  	 	 		\textit{WHO}  		2012.  	 
\bibitem[Universal access to safe blood transfusion, global strategic plan ()]{b24}\label{b24} 	 		\textit{Universal access to safe blood transfusion, global strategic plan},  		 \url{http://www.who.int/bloodsafety/global\textunderscore database/en}  		2008-2015.  		 			WHO 		 	 
\bibitem[WHO, blood transfusion safety ()]{b25}\label{b25} 	 		 \url{http://www.who.int/bloodsafety/global\textunderscore database/en}  	 	 		\textit{WHO, blood transfusion safety},  				1998 -1999.  	 
\bibitem[WHO, Global Database on Blood Safety in Summary Report ()]{b18}\label{b18} 	 		 \url{http://www.who.int/bloodsafety}  		\textit{WHO, Global Database on Blood Safety in Summary Report},  				2011.  	 	 (blood safety indicators (GDBS) 
\bibitem[WHO, Global Database on Blood Safety Reports ()]{b14}\label{b14} 	 		 \url{http://www.who.int/bloodsafety}  		\textit{WHO, Global Database on Blood Safety Reports},  				2001-2002.  	 
\bibitem[WHO, Global Database on Blood Safety, 2011, and blood safety indicators (GDBS) available from]{b13}\label{b13} 	 		 \url{http://www.who.int/bloodsafety}  		\textit{WHO, Global Database on Blood Safety, 2011, and blood safety indicators (GDBS) available from},  				 	 
\bibitem[WHO/world-blooddonor-day ()]{b11}\label{b11} 	 		\textit{WHO/world-blooddonor-day},  		 \url{http://www.who.int/campaigns/world-blood-donor-day/2013/en/index.html}  		2013.  	 
\end{bibitemlist}
 			 		 	 
\end{document}
