# Introduction he main purpose of this study was to evaluatea new method for obtaining atrichromic staining faster and effective for it used the samedyesand proce-ededim plementing two different technical koplicone with and one with direct drops of reagent in the lamina. Author: Bacteriologa, Universidad Colegio Mayor de Cundinamarca. Colombia, Afiliation: CMD SIPLAS. e-mail: daissyjasbeydi@gmail.com There were 20 positive smearsall parasites and made several technical modifications in order to simplify and expedite the procedure equally maintaining the excellent staining qualities, the nimplemented the steps mentioned in the original technique and then the technique modified. ethanol should be as free of water as possible to avoid both the reactive evaporation of moisture absorption as that can preventeasy identification of the parasite.( 2) # Original Technical Steps # Wheatley's Modification of the Gomori Note: formalin fixed Fecal samples are suitable for this dyeing process # a) Important considerations The fund continues to see green and cytoplasm of protozoa is stained a blue green and purple. There dnuclei with inclusions purple and intracellular structures are easy to distinguish as glycogen vacuoles are the Iodamoeba butschlii. ( 6 Kappa: the agreement between observers for the identification of parasitic forms, leukocytes, yeasts, and negative for themis 1.0, which shows diagnostic accuracy and level of agreement between observers for the samples with the latest changes made by SIPLAS medical laboratory, concluding that the changes mentioned here allow adequate identification of both parasite forms leukocytes, yeast and other fungal forms structures that allow the definition diagnosed patients, ensuring diagnostic accuracy versus the clinical definition kappa Degree of agreement < 0 without agreement 0 -0. # Sensitivity and Specificity The sensitivity and specificity of the samples analyzed for fungal structures, yeast and parasiticleucoidesis 100%, which shows that the stain can classify patients according to the irpositive or negative real state against it sclinical definition # a) Parasitic forms identification with modified technique Micrographs of amoebae obtained modified tech-nique implemented 1![Figure 1 : Cysts Blastocystis hominis](image-2.png "Figure 1 :") 2![Figure 2 : Cysts Entamoeba coli](image-3.png "Figure 2 :") 3![Figure 3 : Cysts Iodamoeba butschlii](image-4.png "Figure 3 :") 4![Figure 4 : Cysts Endolimax nana](image-5.png "Figure 4 :") 5![Figure 5 : Cysts Giardia Duodenalis Note: The photomicrographs were taken by the bacteriologys Daissy Vargas Sepulveda in CMD SIPLAS IV.](image-6.png "Figure 5 :Conclusions?") 1Experimental developmentValidation of the art Stian Modified Trichrome in CmdSiplas DEb) Kappa index leukocytesTABLA DE 2*2ReferenceNo referenceReagentReagentOBSERVER 1 Reagent to validate VpFpconcordance No Reagent to validate FNVNin identification Vp+FNnegative VN+FpOBSERVADOR 2concordance in identifying parasitic forms Sensitivity Specificity negativef l k 1 Vp/(Vp+FN)=True positives t 0 VN/(VN+Fp)=True Negatives CRITERIO DE 0 D 16 ACEPTABILIDACRITERIO DE ACEPTABILIDAD217 %Sensitivity ÍNDICE KAPPA VPP (%)1100,016 1,00 100,0DIAGNOSTICACCURACYIS NOTED%Specificity VNP (%)100,0100,0Total Positives1Volume XIII Issue VII Version ITABLA DE 2*2 Reference Reagent Reagent to validate Vp Vp+FN Sensitivity Vp/(Vp+FN)=True positives No reference Reagent Fp VN+Fp Specificity VN/(VN+Fp)=True Negatives Total Negatives 16 ÍNDICE KAPPA Pe 0,003 No Reagent to validate FN VN Po 1,00( ) K2*2 Reference Reagent ÍNDICE KAPPA Reagent to validate Vp VPP (%)No reference Reagent 1,00 Fp 100,0Aceptability DIAGNOSTICACCURACYIS NOTEDNo Reagent to VNP (%) validate Total positives FN100,0 VN 1Vp+FN Total NegativesVN+Fp 16SensitivityVp/(Vp+FN)=True positivesSpecificityVN/(VN+Fp)=True Negatives ÍNDICE KAPPAPe0,003Po1,00Acceptabilityc) Kappa index yeastÍNDICE KAPPA1,00DIAGNOSTICACCURACYIS NOTEDOBSERVER 1VPP (%)100,0 concordance inVNP (%)100,0 identification innegativeBSERVADOR 2Total positives Total Negatives Pe ÍNDICE KAPPA yeast 16 1 0,886 concordance in identifying parasitic forms 1 negative 00 1617Po11,0016%Sensitivity100,0%Specificity100,0 © 2013 Global Journals Inc. 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