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\setlength{\parsep}{2pt}% \def\makelabel##1{\upshape ##1}}% } {\end{list}\end{raggedright}} \newenvironment{specHead}[2]% {\vspace{20pt}\hrule\vspace{10pt}% \phantomsection\label{#1}\markright{#2}% \pdfbookmark[2]{#2}{#1}% \hspace{-0.75in}{\bfseries\fontsize{16pt}{18pt}\selectfont#2}% }{} \def\TheFullDate{2014-01-15 (revised: 15 January 2014)} \def\TheID{\makeatother } \def\TheDate{2014-01-15} \title{Determination of the Causative Agents of Bacteremia in Children under 5 Years and their Susceptibility Pattern to the Antibiotics} \author{}\makeatletter \makeatletter \newcommand*{\cleartoleftpage}{% \clearpage \if@twoside \ifodd\c@page \hbox{}\newpage \if@twocolumn \hbox{}\newpage \fi \fi \fi } \makeatother \makeatletter \thispagestyle{empty} \markright{\@title}\markboth{\@title}{\@author} \renewcommand\small{\@setfontsize\small{9pt}{11pt}\abovedisplayskip 8.5\p@ plus3\p@ minus4\p@ \belowdisplayskip \abovedisplayskip \abovedisplayshortskip \z@ plus2\p@ \belowdisplayshortskip 4\p@ 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\setlength{\parindent}{0pt}\raggedright \textcolor{GJMediumGrey}{\rule{\textwidth}{2pt}} \vskip16pt \textcolor{GJBlue}{\large\bfseries\abstractname\space} }{% \vskip8pt \textcolor{GJMediumGrey}{\rule{\textwidth}{2pt}} \vskip16pt } \usepackage[absolute,overlay]{textpos} \makeatother \usepackage{lineno} \linenumbers \begin{document} \author[1]{Dr. Abhineet Mehrotra} \author[2]{Dr. Shailendra Mishra} \affil[1]{ Career Institute of Medical Sciences, Lucknow, UP, India} \renewcommand\Authands{ and } \date{\small \em Received: 14 December 2013 Accepted: 5 January 2014 Published: 15 January 2014} \maketitle \begin{abstract} Objective: To determine the causative agents of bacteremia in children under 5 years and their susceptibility to the commonly used antibiotics. Methods: This was a cross-sectional study on children (aged from 1 day to 5 years) admitted to the paediatric ward. The patients included all newborn babies and children admitted with fever and suspected of having sepsis. All the included children were clinically diagnosed for septicemia following strict aseptic precautions and the blood sample was taken. Blood culture were done by standard method. Results: The overall incidence of bacteremia was 23.1%. The incidence of bacteremia was higher among the chidren of age group 13-60 month (38.1%) than <1 month (23.4%) and 1-12 month (12.5%). The male (25.8%) children were affected than females (19.6%). \end{abstract} \keywords{bacteremia, incidence, children, bacterial isolates.} \begin{textblock*}{18cm}(1cm,1cm) % {block width} (coords) \textcolor{GJBlue}{\LARGE Global Journals \LaTeX\ JournalKaleidoscope\texttrademark} \end{textblock*} \begin{textblock*}{18cm}(1.4cm,1.5cm) % {block width} (coords) \textcolor{GJBlue}{\footnotesize \\ Artificial Intelligence formulated this projection for compatibility purposes from the original article published at Global Journals. However, this technology is currently in beta. \emph{Therefore, kindly ignore odd layouts, missed formulae, text, tables, or figures.}} \end{textblock*} \let\tabcellsep& \section[{Introduction}]{Introduction}\par acteremia is the presence of viable bacteria in the circulating blood. Bacteria may enter the blood stream giving rise to bacteremia from an existing focus of infection from a site with the commensally flora or by direct inoculation of contaminated materials into the vascular system. These organisms are often cleared from the blood within minutes, so the bacteremia is silent and transient, but if the immune system is overwhelmed or evaded, organisms persist in the blood and bacterimic symptoms would arise. Bacteremia should be distinguished from septicemia in which signs and symptoms of severe diseases are present \hyperref[b0]{1} . Neonates are particularly vulnerable to infections because of their weak immune barrier. Several risk factors have been identified both in the neonates and children which makes them susceptible to infections \hyperref[b1]{2} . Children with septicaemia present with fever, difficulty in breathing, tachycardia, malaise, refusal of feeds or lethargy \hyperref[b2]{3} . Studies of bloodstream infections in children admitted to African hospitals suggest that the prevalence of bacterial bloodstream infections among inpatients with fever or clinical sepsis exceeds that described in wealthier regions \hyperref[b3]{4,}\hyperref[b4]{5} . Bloodstream infections continue to be the major cause of morbidity and mortality despite advance in antimicrobial therapy and supportive care \hyperref[b5]{6} . Fever in infants younger than 1 year old, especially those younger than 3 months, can signal a serious infection \hyperref[b6]{7} .\par The aim of this study was to determine the causative agents of bacteremia in children under 5 years and their susceptibility to the commonly used antibiotics. \section[{II.}]{II.} \section[{Material and Methods}]{Material and Methods}\par This was a cross-sectional study approved by the ethical committee of the institute. In this study, 117 blood samples were collected from children (aged from 1 day to 5 years) admitted to the paediatric ward of a teaching hospital in north India. The patients included all newborn babies and children admitted with fever and suspected of having sepsis. Children with fever less than 5 days and with known clinical condition such as malignancies, tuberculosis etc. were excluded from the study. The consent was taken from parent/guardian of each children before enrolling in the study.\par A total of 128 children were included in this study. All the included children were clinically diagnosed for septicemia following strict aseptic precautions and the blood sample was taken. One milliliter (neonates) and 5 ml (children) blood were collected, inoculated into 10 ml and 50 ml, respectively of brain heart infusion broth. The culture bottles were incubated at 37°C aerobically and periodic subcultures were done onto Mac Conkey's agar, blood agar and chocolate agar after overnight incubation on day three, day four and finally on day seven \hyperref[b7]{8} . The growth obtained was identified by conventional biochemical tests and the antibiotic sensitivity testing was performed on Mueller-Hinton agar plates by Kirby-Bauer disc diffusion method. Zone Methods: This was a cross-sectional study on children (aged from 1 day to 5 years) admitted to the paediatric ward. The patients included all newborn babies and children admitted with fever and suspected of having sepsis. All the included children were clinically diagnosed for septicemia following strict aseptic precautions and the blood sample was taken. Blood culture were done by standard method.\par Results: The overall incidence of bacteremia was 23.1\%. The incidence of bacteremia was higher among the chidren of age group 13-60 month (38.1\%) than <1 month (23.4\%) and 1-12 month (12.5\%). The male (25.8\%) children were affected than females (19.6\%). The Klebsiella species organism was the most common organism which was 44.4\% followed by Staphylococcus. Aureus (14.8\%) and Coagulase Negative staphylococci (CONS) (11.1\%). The percentage of other organism was less than 10\%. The percentage of gram negative bacterial isolate was among 70.4\% of the samples and gram negative was 29.6\%. \section[{Conclusion:}]{Conclusion:}\par The resistance of the recovered Klebsiella spp.\par isolates to a number of antimicrobial agents was determined, and a pattern of multi-resistance was observed which may explain the prevalence of these isolates in pediatric bacteremia.\par diameter was measured and interpreted as per the Clinical and Laboratory Standards Institute (CLSI) guidelines as used by Tiwari et al \hyperref[b7]{8} . Bacterial sensitivity was tested for the following antimicrobials: amikacin, amoxicillin clavulanic acid, ampicillin, aztreonam, cefotaxime, ceftazidime, ceftriaxone, cephalexin, cefoxitin, ciprofloxacin, gentamicin, imipenem, meropenem, piperacillin-tazobactam, tobramycin, linezolid and vancomycin.\par Methicillin resistance in Staphylococcus aureus (MRSA) was tested using Mueller-Hinton agar with 4\% NaCl with cefoxitin disc (30 micrograms) by Kirby-Bauer disc diffusion method. A zone size of >22 mm was considered sensitive and < 21 was considered as resistant.\par Suspected extended-spectrum beta lactamases (ESBLs) producing organisms were confirmed by double disk synergy test \hyperref[b7]{8} . Detection of plasmid-mediated AmpC was done by the AmpC disk test and the isolates showing reduced susceptibility to carbapenems (imipenem and meropenem) were selected for detection of metallo-beta lactamases (MBLs) enzymes by imipenem-EDTA disk method. For quality control of disc diffusion tests ATCC control strains of E. coli ATCC 25922, S. aureus ATCC 25923 and P. aeruginosa ATCC 27853 strains were used. The results were expressed as percentages. Microsoft excel was used for the interpretation of these results. \section[{III.}]{III.} \section[{Results}]{Results}\par Table-1 presents the percentage of children of Bacteremia according to demographic profile. The overall incidence of bacteremia was 23.1\%. The incidence of bacteremia was higher among the chidren of age group 13-60 month (38.1\%) than <1 month (23.4\%) and 1-12 month (12.5\%). The male (25.8\%) children were affected than females (19.6\%).\par The Klebsiella species organism was the most common organism which was 44.4\% followed by Staphylococcus. Aureus (14.8\%) and Coagulase Negative staphylococci (CONS) (11.1\%). The percentage of other organism was less than 10\% (Fig. {\ref 1}).\par The percentage of gram negative bacterial isolate was among 70.4\% of the samples and gram negative was 29.6\% (Fig. {\ref 2}).\par Table-2 depicts antibacterial resistance pattern of the gram negative blood stream isolates. Klebsiella spp organism was resistance to ampicillin (3), amoxyclav (5), amikacin \& gentamycin (2) and one each tocotrimoxazole, ciprofloxacin, cefotaxime\& aztreonam. The frequency of other organism resistance to most of drugs was one.The frequency gram positive isolates resistance to most of the drugs was one (Table-3). \section[{IV.}]{IV.} \section[{Discussion}]{Discussion}\par Who guidelines for management of acute illness in children (Integrated Management of Childhood Illness) recommend use of an appropriate antibacterial drug in addition to an antimalarial drug in children with certain signs of severe illness \hyperref[b8]{9} . Despite considerable progress in hygiene, antimicrobial therapy, and supportive treatment, blood stream infections remain important causes of morbidity and mortality which, may reaches to 20\%-30\% \hyperref[b9]{10} . Microbiologic culturing of blood is the only available means for diagnosis of these infections and allows for successful recovery of bacteria in 99\% in patients with bacteremia of septicemia \hyperref[b10]{11} . An American review covering a 50-years period has show major changes in the etiology of neonatal septicemia \hyperref[b12]{12} .\par In this study, the incidence of bacteremia was higher among male children. Similar findings had been reported in southern state of India \hyperref[b7]{8} . Some other studies had also reported higher incidence of bacteremia in male children \hyperref[b13]{13,}\hyperref[b1]{2} . The incidence of bacteremia was higher among 13-60 month (38.1\%) in this study which was contradictory to the studies by Tsering et al \hyperref[b14]{14} and Meremkwer et al. \hyperref[b1]{2} in which incidence of bacteremia was more common among newborns. In this study, the culture positivity rate was found to be 23.1\% (23/117). Almost similar rate had been reported in a Indian study in which blood culture positivity rate of 25\% \hyperref[b7]{8} . Other studies had also reported similar positivity rate \hyperref[b15]{15,}\hyperref[b14]{14} .\par The percentage of gram negative organism found in this study was similar to other studies also \hyperref[b16]{16,}\hyperref[b15]{15} .\par In the present study, the Klebsiella species was the commonest isolate associated with bacteremia which was similar to study by Al-Charrakh et al \hyperref[b17]{17} . Many studies have been shown that Gram positive organisms were the mainly Staphylococcus aureus as the most frequently isolated bacteria causing bacteremia \hyperref[b18]{18,}\hyperref[b19]{19} .\par There was varying number of the gram negative and positive organisms resistance to different drugs in this study. Prabhu et al \hyperref[b20]{20} reported that the gram negative organisms showed maximum resistance to ampicillin. However, Tiwari et al \hyperref[b7]{8} reported that the gram positive organisms showed 77.78\% resistance to penicillin but were 100\% sensitive to linezolid and vancomycin. Among the 6 Staphylococcus aureus, 2(33.33\%) were detected as Methicillin resistant Staphylococcus aureus (MRSA).\par One of the limitation of this study is lesser sample size. Studies on larger sample size is recommended for better interpretation of the results. The studies on the community acquired blood stream infections is also needed at present. \section[{V.}]{V.} \section[{Conclusion}]{Conclusion}\par The resistance of the recovered Klebsiella spp. isolates to a number of antimicrobial agents was determined, and a pattern of multiresistance was observed which may explain the prevalence of these isolates in pediatric bacteremia. \section[{Source of funding: None}]{Source of funding: None}\begin{figure}[htbp] \noindent\textbf{}\includegraphics[]{image-2.png} \caption{\label{fig_0}B}\end{figure} \begin{figure}[htbp] \noindent\textbf{1} \par \begin{longtable}{P{0.3471830985915493\textwidth}P{0.20950704225352113\textwidth}P{0.13767605633802818\textwidth}P{0.15563380281690142\textwidth}} \tabcellsep No. of\tabcellsep \multicolumn{2}{l}{Bacteremia}\\ \tabcellsep children\tabcellsep No.\tabcellsep \%\\ \tabcellsep assessed\tabcellsep \tabcellsep \\ Age group\tabcellsep \tabcellsep \tabcellsep \\ <1 month\tabcellsep 64\tabcellsep 15\tabcellsep 23.4\\ 1-12\tabcellsep 32\tabcellsep 4\tabcellsep \\ months\tabcellsep \tabcellsep \tabcellsep 12.5\\ 13-60\tabcellsep 21\tabcellsep 8\tabcellsep \\ month\tabcellsep \tabcellsep \tabcellsep 38.1\\ Gender\tabcellsep \tabcellsep \tabcellsep \\ Male\tabcellsep 66\tabcellsep 17\tabcellsep 25.8\\ Female\tabcellsep 51\tabcellsep 10\tabcellsep 19.6\\ Total\tabcellsep 117\tabcellsep 27\tabcellsep 23.1\end{longtable} \par \caption{\label{tab_0}Table 1 :}\end{figure} \begin{figure}[htbp] \noindent\textbf{2} \par \begin{longtable}{P{0.13399014778325122\textwidth}P{0.05862068965517241\textwidth}P{0.025123152709359605\textwidth}P{0.11724137931034483\textwidth}P{0.037684729064039406\textwidth}P{0.07536945812807881\textwidth}P{0.012561576354679803\textwidth}P{0.13399014778325122\textwidth}P{0.012561576354679803\textwidth}P{0.06699507389162561\textwidth}P{0.029310344827586206\textwidth}P{0.11724137931034483\textwidth}P{0.029310344827586206\textwidth}} \multicolumn{3}{l}{Antibiotics Klebsiella}\tabcellsep \multicolumn{4}{l}{E.coli (n=2) Pseudomonas}\tabcellsep \multicolumn{2}{l}{Acinetobacter}\tabcellsep S.typhi\tabcellsep \tabcellsep \multicolumn{2}{l}{Citrobacter}\\ \tabcellsep \multicolumn{2}{l}{spp (n=12)}\tabcellsep \tabcellsep \tabcellsep \multicolumn{2}{l}{aeruginosa}\tabcellsep \multicolumn{2}{l}{baumanii (n=1)}\tabcellsep (n=2)\tabcellsep \tabcellsep \multicolumn{2}{l}{freundii}\\ \tabcellsep \tabcellsep \tabcellsep \tabcellsep \tabcellsep (n=2)\tabcellsep \tabcellsep \tabcellsep \tabcellsep \tabcellsep \tabcellsep (n=1)\\ \tabcellsep No.\tabcellsep \%\tabcellsep No.\tabcellsep \%\tabcellsep No.\tabcellsep \%\tabcellsep No.\tabcellsep \%\tabcellsep No.\tabcellsep \%\tabcellsep No.\tabcellsep \%\\ Ampicillin\tabcellsep 3\tabcellsep 25.0\tabcellsep 1\tabcellsep \multicolumn{2}{l}{50.0 NT}\tabcellsep -\tabcellsep NT\tabcellsep -\tabcellsep 2\tabcellsep 100.\tabcellsep 1\tabcellsep 100.0\\ \tabcellsep \tabcellsep \tabcellsep \tabcellsep \tabcellsep \tabcellsep \tabcellsep \tabcellsep \tabcellsep \tabcellsep 0\tabcellsep \end{longtable} \par \caption{\label{tab_1}Table 2 :}\end{figure} \begin{figure}[htbp] \noindent\textbf{3} \par \begin{longtable}{P{0.3439306358381503\textwidth}P{0.17196531791907516\textwidth}P{0.12283236994219654\textwidth}P{0.0884393063583815\textwidth}P{0.12283236994219654\textwidth}} Antibiotics\tabcellsep Staphylococcus\tabcellsep \tabcellsep CONS\tabcellsep \\ \tabcellsep aureus (n=4)\tabcellsep \tabcellsep (n=3)\tabcellsep \\ \tabcellsep No.\tabcellsep \%\tabcellsep No.\tabcellsep \%\\ Penicillin\tabcellsep 2\tabcellsep 50.0\tabcellsep 1\tabcellsep 33.3\\ Amoxyclav\tabcellsep 1\tabcellsep 25.0\tabcellsep 1\tabcellsep 33.3\\ Cefoxitin\tabcellsep 1\tabcellsep 25.0\tabcellsep 2\tabcellsep 66.7\\ Erythromycin\tabcellsep 2\tabcellsep 50.0\tabcellsep 1\tabcellsep 33.3\\ Linezolid\tabcellsep 1\tabcellsep 25.0\tabcellsep 0\tabcellsep 0.0\\ Vancomycin\tabcellsep 0\tabcellsep 0.0\tabcellsep 1\tabcellsep 33.3\end{longtable} \par \caption{\label{tab_2}Table 3 :}\end{figure} \footnote{© 2014 Global Journals Inc. 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