\documentclass[11pt,twoside]{article}\makeatletter

\IfFileExists{xcolor.sty}%
  {\RequirePackage{xcolor}}%
  {\RequirePackage{color}}
\usepackage{colortbl}
\usepackage{wrapfig}
\usepackage{ifxetex}
\ifxetex
  \usepackage{fontspec}
  \usepackage{xunicode}
  \catcode`⃥=\active \def⃥{\textbackslash}
  \catcode`❴=\active \def❴{\{}
  \catcode`❵=\active \def❵{\}}
  \def\textJapanese{\fontspec{Noto Sans CJK JP}}
  \def\textChinese{\fontspec{Noto Sans CJK SC}}
  \def\textKorean{\fontspec{Noto Sans CJK KR}}
  \setmonofont{DejaVu Sans Mono}
  
\else
  \IfFileExists{utf8x.def}%
   {\usepackage[utf8x]{inputenc}
      \PrerenderUnicode{–}
    }%
   {\usepackage[utf8]{inputenc}}
  \usepackage[english]{babel}
  \usepackage[T1]{fontenc}
  \usepackage{float}
  \usepackage[]{ucs}
  \uc@dclc{8421}{default}{\textbackslash }
  \uc@dclc{10100}{default}{\{}
  \uc@dclc{10101}{default}{\}}
  \uc@dclc{8491}{default}{\AA{}}
  \uc@dclc{8239}{default}{\,}
  \uc@dclc{20154}{default}{ }
  \uc@dclc{10148}{default}{>}
  \def\textschwa{\rotatebox{-90}{e}}
  \def\textJapanese{}
  \def\textChinese{}
  \IfFileExists{tipa.sty}{\usepackage{tipa}}{}
\fi
\def\exampleFont{\ttfamily\small}
\DeclareTextSymbol{\textpi}{OML}{25}
\usepackage{relsize}
\RequirePackage{array}
\def\@testpach{\@chclass
 \ifnum \@lastchclass=6 \@ne \@chnum \@ne \else
  \ifnum \@lastchclass=7 5 \else
   \ifnum \@lastchclass=8 \tw@ \else
    \ifnum \@lastchclass=9 \thr@@
   \else \z@
   \ifnum \@lastchclass = 10 \else
   \edef\@nextchar{\expandafter\string\@nextchar}%
   \@chnum
   \if \@nextchar c\z@ \else
    \if \@nextchar l\@ne \else
     \if \@nextchar r\tw@ \else
   \z@ \@chclass
   \if\@nextchar |\@ne \else
    \if \@nextchar !6 \else
     \if \@nextchar @7 \else
      \if \@nextchar (8 \else
       \if \@nextchar )9 \else
  10
  \@chnum
  \if \@nextchar m\thr@@\else
   \if \@nextchar p4 \else
    \if \@nextchar b5 \else
   \z@ \@chclass \z@ \@preamerr \z@ \fi \fi \fi \fi
   \fi \fi  \fi  \fi  \fi  \fi  \fi \fi \fi \fi \fi \fi}
\gdef\arraybackslash{\let\\=\@arraycr}
\def\@textsubscript#1{{\m@th\ensuremath{_{\mbox{\fontsize\sf@size\z@#1}}}}}
\def\Panel#1#2#3#4{\multicolumn{#3}{){\columncolor{#2}}#4}{#1}}
\def\abbr{}
\def\corr{}
\def\expan{}
\def\gap{}
\def\orig{}
\def\reg{}
\def\ref{}
\def\sic{}
\def\persName{}\def\name{}
\def\placeName{}
\def\orgName{}
\def\textcal#1{{\fontspec{Lucida Calligraphy}#1}}
\def\textgothic#1{{\fontspec{Lucida Blackletter}#1}}
\def\textlarge#1{{\large #1}}
\def\textoverbar#1{\ensuremath{\overline{#1}}}
\def\textquoted#1{‘#1’}
\def\textsmall#1{{\small #1}}
\def\textsubscript#1{\@textsubscript{\selectfont#1}}
\def\textxi{\ensuremath{\xi}}
\def\titlem{\itshape}
\newenvironment{biblfree}{}{\ifvmode\par\fi }
\newenvironment{bibl}{}{}
\newenvironment{byline}{\vskip6pt\itshape\fontsize{16pt}{18pt}\selectfont}{\par }
\newenvironment{citbibl}{}{\ifvmode\par\fi }
\newenvironment{docAuthor}{\ifvmode\vskip4pt\fontsize{16pt}{18pt}\selectfont\fi\itshape}{\ifvmode\par\fi }
\newenvironment{docDate}{}{\ifvmode\par\fi }
\newenvironment{docImprint}{\vskip 6pt}{\ifvmode\par\fi }
\newenvironment{docTitle}{\vskip6pt\bfseries\fontsize{22pt}{25pt}\selectfont}{\par }
\newenvironment{msHead}{\vskip 6pt}{\par}
\newenvironment{msItem}{\vskip 6pt}{\par}
\newenvironment{rubric}{}{}
\newenvironment{titlePart}{}{\par }

\newcolumntype{L}[1]{){\raggedright\arraybackslash}p{#1}}
\newcolumntype{C}[1]{){\centering\arraybackslash}p{#1}}
\newcolumntype{R}[1]{){\raggedleft\arraybackslash}p{#1}}
\newcolumntype{P}[1]{){\arraybackslash}p{#1}}
\newcolumntype{B}[1]{){\arraybackslash}b{#1}}
\newcolumntype{M}[1]{){\arraybackslash}m{#1}}
\definecolor{label}{gray}{0.75}
\def\unusedattribute#1{\sout{\textcolor{label}{#1}}}
\DeclareRobustCommand*{\xref}{\hyper@normalise\xref@}
\def\xref@#1#2{\hyper@linkurl{#2}{#1}}
\begingroup
\catcode`\_=\active
\gdef_#1{\ensuremath{\sb{\mathrm{#1}}}}
\endgroup
\mathcode`\_=\string"8000
\catcode`\_=12\relax

\usepackage[a4paper,twoside,lmargin=1in,rmargin=1in,tmargin=1in,bmargin=1in,marginparwidth=0.75in]{geometry}
\usepackage{framed}

\definecolor{shadecolor}{gray}{0.95}
\usepackage{longtable}
\usepackage[normalem]{ulem}
\usepackage{fancyvrb}
\usepackage{fancyhdr}
\usepackage{graphicx}
\usepackage{marginnote}

\renewcommand{\@cite}[1]{#1}


\renewcommand*{\marginfont}{\itshape\footnotesize}

\def\Gin@extensions{.pdf,.png,.jpg,.mps,.tif}

  \pagestyle{fancy}

\usepackage[pdftitle={Seroprevalence of Toxoplasma Gondii and Neospora Caninum Infection in Cattle in Grenada, West Indies},
 pdfauthor={}]{hyperref}
\hyperbaseurl{}

	 \paperwidth210mm
	 \paperheight297mm
              
\def\@pnumwidth{1.55em}
\def\@tocrmarg {2.55em}
\def\@dotsep{4.5}
\setcounter{tocdepth}{3}
\clubpenalty=8000
\emergencystretch 3em
\hbadness=4000
\hyphenpenalty=400
\pretolerance=750
\tolerance=2000
\vbadness=4000
\widowpenalty=10000

\renewcommand\section{\@startsection {section}{1}{\z@}%
     {-1.75ex \@plus -0.5ex \@minus -.2ex}%
     {0.5ex \@plus .2ex}%
     {\reset@font\Large\bfseries}}
\renewcommand\subsection{\@startsection{subsection}{2}{\z@}%
     {-1.75ex\@plus -0.5ex \@minus- .2ex}%
     {0.5ex \@plus .2ex}%
     {\reset@font\Large}}
\renewcommand\subsubsection{\@startsection{subsubsection}{3}{\z@}%
     {-1.5ex\@plus -0.35ex \@minus -.2ex}%
     {0.5ex \@plus .2ex}%
     {\reset@font\large}}
\renewcommand\paragraph{\@startsection{paragraph}{4}{\z@}%
     {-1ex \@plus-0.35ex \@minus -0.2ex}%
     {0.5ex \@plus .2ex}%
     {\reset@font\normalsize}}
\renewcommand\subparagraph{\@startsection{subparagraph}{5}{\parindent}%
     {1.5ex \@plus1ex \@minus .2ex}%
     {-1em}%
     {\reset@font\normalsize\bfseries}}


\def\l@section#1#2{\addpenalty{\@secpenalty} \addvspace{1.0em plus 1pt}
 \@tempdima 1.5em \begingroup
 \parindent \z@ \rightskip \@pnumwidth 
 \parfillskip -\@pnumwidth 
 \bfseries \leavevmode #1\hfil \hbox to\@pnumwidth{\hss #2}\par
 \endgroup}
\def\l@subsection{\@dottedtocline{2}{1.5em}{2.3em}}
\def\l@subsubsection{\@dottedtocline{3}{3.8em}{3.2em}}
\def\l@paragraph{\@dottedtocline{4}{7.0em}{4.1em}}
\def\l@subparagraph{\@dottedtocline{5}{10em}{5em}}
\@ifundefined{c@section}{\newcounter{section}}{}
\@ifundefined{c@chapter}{\newcounter{chapter}}{}
\newif\if@mainmatter 
\@mainmattertrue
\def\chaptername{Chapter}
\def\frontmatter{%
  \pagenumbering{roman}
  \def\thechapter{\@roman\c@chapter}
  \def\theHchapter{\roman{chapter}}
  \def\thesection{\@roman\c@section}
  \def\theHsection{\roman{section}}
  \def\@chapapp{}%
}
\def\mainmatter{%
  \cleardoublepage
  \def\thechapter{\@arabic\c@chapter}
  \setcounter{chapter}{0}
  \setcounter{section}{0}
  \pagenumbering{arabic}
  \setcounter{secnumdepth}{6}
  \def\@chapapp{\chaptername}%
  \def\theHchapter{\arabic{chapter}}
  \def\thesection{\@arabic\c@section}
  \def\theHsection{\arabic{section}}
}
\def\backmatter{%
  \cleardoublepage
  \setcounter{chapter}{0}
  \setcounter{section}{0}
  \setcounter{secnumdepth}{2}
  \def\@chapapp{\appendixname}%
  \def\thechapter{\@Alph\c@chapter}
  \def\theHchapter{\Alph{chapter}}
  \appendix
}
\newenvironment{bibitemlist}[1]{%
   \list{\@biblabel{\@arabic\c@enumiv}}%
       {\settowidth\labelwidth{\@biblabel{#1}}%
        \leftmargin\labelwidth
        \advance\leftmargin\labelsep
        \@openbib@code
        \usecounter{enumiv}%
        \let\p@enumiv\@empty
        \renewcommand\theenumiv{\@arabic\c@enumiv}%
	}%
  \sloppy
  \clubpenalty4000
  \@clubpenalty \clubpenalty
  \widowpenalty4000%
  \sfcode`\.\@m}%
  {\def\@noitemerr
    {\@latex@warning{Empty `bibitemlist' environment}}%
    \endlist}

\def\tableofcontents{\section*{\contentsname}\@starttoc{toc}}
\parskip0pt
\parindent1em
\def\Panel#1#2#3#4{\multicolumn{#3}{){\columncolor{#2}}#4}{#1}}
\newenvironment{reflist}{%
  \begin{raggedright}\begin{list}{}
  {%
   \setlength{\topsep}{0pt}%
   \setlength{\rightmargin}{0.25in}%
   \setlength{\itemsep}{0pt}%
   \setlength{\itemindent}{0pt}%
   \setlength{\parskip}{0pt}%
   \setlength{\parsep}{2pt}%
   \def\makelabel##1{\itshape ##1}}%
  }
  {\end{list}\end{raggedright}}
\newenvironment{sansreflist}{%
  \begin{raggedright}\begin{list}{}
  {%
   \setlength{\topsep}{0pt}%
   \setlength{\rightmargin}{0.25in}%
   \setlength{\itemindent}{0pt}%
   \setlength{\parskip}{0pt}%
   \setlength{\itemsep}{0pt}%
   \setlength{\parsep}{2pt}%
   \def\makelabel##1{\upshape ##1}}%
  }
  {\end{list}\end{raggedright}}
\newenvironment{specHead}[2]%
 {\vspace{20pt}\hrule\vspace{10pt}%
  \phantomsection\label{#1}\markright{#2}%

  \pdfbookmark[2]{#2}{#1}%
  \hspace{-0.75in}{\bfseries\fontsize{16pt}{18pt}\selectfont#2}%
  }{}
      \def\TheFullDate{2014-01-15 (revised: 15 January 2014)}
\def\TheID{\makeatother }
\def\TheDate{2014-01-15}
\title{Seroprevalence of Toxoplasma Gondii and Neospora Caninum Infection in Cattle in Grenada, West Indies}
\author{}\makeatletter 
\makeatletter
\newcommand*{\cleartoleftpage}{%
  \clearpage
    \if@twoside
    \ifodd\c@page
      \hbox{}\newpage
      \if@twocolumn
        \hbox{}\newpage
      \fi
    \fi
  \fi
}
\makeatother
\makeatletter
\thispagestyle{empty}
\markright{\@title}\markboth{\@title}{\@author}
\renewcommand\small{\@setfontsize\small{9pt}{11pt}\abovedisplayskip 8.5\p@ plus3\p@ minus4\p@
\belowdisplayskip \abovedisplayskip
\abovedisplayshortskip \z@ plus2\p@
\belowdisplayshortskip 4\p@ plus2\p@ minus2\p@
\def\@listi{\leftmargin\leftmargini
               \topsep 2\p@ plus1\p@ minus1\p@
               \parsep 2\p@ plus\p@ minus\p@
               \itemsep 1pt}
}
\makeatother
\fvset{frame=single,numberblanklines=false,xleftmargin=5mm,xrightmargin=5mm}
\fancyhf{} 
\setlength{\headheight}{14pt}
\fancyhead[LE]{\bfseries\leftmark} 
\fancyhead[RO]{\bfseries\rightmark} 
\fancyfoot[RO]{}
\fancyfoot[CO]{\thepage}
\fancyfoot[LO]{\TheID}
\fancyfoot[LE]{}
\fancyfoot[CE]{\thepage}
\fancyfoot[RE]{\TheID}
\hypersetup{citebordercolor=0.75 0.75 0.75,linkbordercolor=0.75 0.75 0.75,urlbordercolor=0.75 0.75 0.75,bookmarksnumbered=true}
\fancypagestyle{plain}{\fancyhead{}\renewcommand{\headrulewidth}{0pt}}

\date{}
\usepackage{authblk}

\providecommand{\keywords}[1]
{
\footnotesize
  \textbf{\textit{Index terms---}} #1
}

\usepackage{graphicx,xcolor}
\definecolor{GJBlue}{HTML}{273B81}
\definecolor{GJLightBlue}{HTML}{0A9DD9}
\definecolor{GJMediumGrey}{HTML}{6D6E70}
\definecolor{GJLightGrey}{HTML}{929497} 

\renewenvironment{abstract}{%
   \setlength{\parindent}{0pt}\raggedright
   \textcolor{GJMediumGrey}{\rule{\textwidth}{2pt}}
   \vskip16pt
   \textcolor{GJBlue}{\large\bfseries\abstractname\space}
}{%   
   \vskip8pt
   \textcolor{GJMediumGrey}{\rule{\textwidth}{2pt}}
   \vskip16pt
}

\usepackage[absolute,overlay]{textpos}

\makeatother 
      \usepackage{lineno}
      \linenumbers
      
\begin{document}

             \author[1]{Ravindra  Sharma}

             \affil[1]{  St Georgeas University}

\renewcommand\Authands{ and }

\date{\small \em Received: 6 December 2013 Accepted: 1 January 2014 Published: 15 January 2014}

\maketitle


\begin{abstract}
        


In view of the limited data on the seroprevalence of Toxoplasma gondii and Neospora caninum in the Caribbean region, this study aimed to estimate the seroprevalence of these parasites in cattle in Grenada, West Indies. In Total 148 serum samples were collected from the jugular veins of cattle from the six parishes in the country. The samples were surveyed for T. gondii and N. caninum antibody by an enzyme-linked immunosorbant assay (ELISA). The overall seroprevalence of T. gondii was 2.7% (4/148) and the seropositivity of N. caninum was 6.8% (10/148). The present results indicate exposure of cattle to T. gondii and N. caninum in Grenada.

\end{abstract}


\keywords{}

\begin{textblock*}{18cm}(1cm,1cm) % {block width} (coords) 
\textcolor{GJBlue}{\LARGE Global Journals \LaTeX\ JournalKaleidoscope\texttrademark}
\end{textblock*}

\begin{textblock*}{18cm}(1.4cm,1.5cm) % {block width} (coords) 
\textcolor{GJBlue}{\footnotesize \\ Artificial Intelligence formulated this projection for compatibility purposes from the original article published at Global Journals. However, this technology is currently in beta. \emph{Therefore, kindly ignore odd layouts, missed formulae, text, tables, or figures.}}
\end{textblock*}


\let\tabcellsep& 	 	 		 
\section[{Introduction}]{Introduction}\par
oxoplasma gondii (T. gondii) is a zoonotic protozoan parasite causing infection in most warm blooded species of animals including humans \hyperref[b8]{(Dubey 2010)}. T. gondii causes major economic losses in livestock through abortions, stillbirths and neonatal losses \hyperref[b7]{(Dubey et al. 2007}). Infection in humans and animals can occur by ingestion of T. gondii oocysts from cats which are the definitive hosts or by consuming raw uncooked meat containing T. gondii tissue cysts, which develop in infected animals \hyperref[b8]{(Dubey 2010)}. Cattle and other herbivorous animals contact the infection from grass and pastures contaminated with cats' feces \hyperref[b11]{(Jacek et al. 2007}). In humans, T. gondii constitutes an important health problem in pregnant women because of the threat of fetal infection and in immunocompromized patients, aggravates existing pathological conditions (Jacek et al. 2007  The objective of this study was to estimate the seroprevalence for T. gondii and N. caninum in cattle in Grenada.\par
II. 
\section[{Materials and Methods}]{Materials and Methods}\par
For the present survey a 2 step (multistage cluster sampling) sampling procedure was adopted. Generally cattle herds in Grenada are small comprising 1-4 animals. In the first step, 35 herds consisting of 6\% of (2500) estimated cattle population in Grenada, were selected randomly from all the six parishes in the country. All herds having less than 10 cattle were sampled. For a herd size greater than 10, 80\% of the animals were sampled. A total of 148 cattle were sampled randomly. Two milliliter of blood from the jugular vein of each animal was obtained. Blood samples were centrifuged at 1500g for 15 minutes, and the serum was collected and stored at -20 0 C until tested for antibodies to T. gondii and N. caninum using commercial ELISA kits (IDvet France). ELISA was performed following the instruction of the manufacturer. 
\section[{III.}]{III.} 
\section[{Results}]{Results}\par
Out of 148 cattle tested 4 animals [2.7\%, 95\% confidence Interval (CI), 0.09\% to 5.31\%] were positive for T. gondii and 10 [6.8\% 95\% confidence interval (CI), 2.74\% to 10.86\%] for C. caninum.\par
Results are presented in table 1. The variation in seroprevalence of T. gondii between various countries may be attributed to the difference in the rate of contamination of the environment with oocysts from cat, the definitive host and differences in management methods (Pet Gondim et al. 1999, Jacek et al. 2007). The low prevalence of T. gondii (2.7\%) could be related to cattle production in Grenada. Cattle herds in Grenada are small compared to more intensely managed herds elsewhere in the world. Small herds get better hygienic conditions and are in less contact with infected cats. Further studies regarding the differences between management practices for cattle in Grenada could potentially shed more light on this topic. This is the first report of N. caninum surveillance in cattle in Grenada. Antibodies to N. caninum was low [6.8\%; 95\% CI, 2.74\% to 10.86\%] in the tested cattle. Low prevalence has also been reported in Germany 4.1\%  {\ref (Conraths et al.1996)}, Canada 8.3\% \hyperref[b23]{(Vanleeuwen et al. 2006)}, and Serbia 15\% \hyperref[b15]{(Kuruca et al. 2013)}. 
\section[{T Global Journal of}]{T Global Journal of}\par
Variations in the seroprevalence of Neosporosis depending on the region, climate and type of serological tests have been reported \hyperref[b7]{(Dubey et al. 2007}). This variation amongst the different countries and regions of the world may be attributed to risk factors like dog density, climatic factors and management practices on the farm. Climatic factors influence the sporulation and survival of oocysts \hyperref[b19]{(Rinaldi et al. 2005}).\par
Variation in seroprevalence with respect to management practices could be attributed to the size of farm (Guimaraes et al. 2004; Rinaldi et al. 2005).\par
A positive relation between seropositivity of farm dogs and bovine neosporosis has been reported by previous researchers  {\ref (Wouda et al.1999;}\hyperref[b12]{Kacar et al. 2012)}). Seroprevalence in cattle is lower where dogs are not present on the farm  {\ref (Basso et al. 2001, Antony and}\hyperref[b0]{Williamson 2003)}. In Grenada seropositivity for N. caninum in dogs has been demonstrated to be low varying from 1.2\% in owned dog to1.6\% in stray dogs (unpublished data). This low seropositivity in dogs correlates well with the lower seropositivity in cattle (6.8\%).\begin{figure}[htbp]
\noindent\textbf{}\includegraphics[]{image-2.png}
\caption{\label{fig_0}}\end{figure}
 \begin{figure}[htbp]
\noindent\textbf{1} \par 
\begin{longtable}{P{0.7565065840433772\textwidth}P{0.027652982184353213\textwidth}P{0.02501936483346243\textwidth}P{0.01646010844306739\textwidth}P{0.01053446940356313\textwidth}P{0.013826491092176606\textwidth}}
\tabcellsep \multicolumn{2}{l}{Toxoplasma gondii}\tabcellsep \multicolumn{3}{l}{Neospora caninum}\\
Number of\tabcellsep Number\tabcellsep \multicolumn{2}{l}{Percent positivity Number of cattle}\tabcellsep Number\tabcellsep Percent positivity\\
cattle tested\tabcellsep positive\tabcellsep \tabcellsep tested\tabcellsep positive\tabcellsep \\
148\tabcellsep 4\tabcellsep 2.7\tabcellsep 148\tabcellsep 10\tabcellsep 6.8\\
IV.\tabcellsep \multicolumn{2}{l}{Discussion}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{In the present survey antibodies to T. gondii}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{were detected in only 4 [2.7\%, 95\% CI, 0.09\% to 5.31\%]}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{of the tested cattle. Chikweto et al. (2011) reported a}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{seroprevalence of T. gondii (8.5\%) in cattle using MAT.}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{In both surveys antibodies to T. gondii in cattle was low.}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{Cattle are considered to be a poor host for T. gondii}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{because of its relative natural resistance to this parasite}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{(Dubey and Thulliez 1994, Pita Gondim et al, 1999,}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{Dubey 2010). Similar to our observations in Grenada,}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{low seroprevalence has been reported from Iran1.6\%}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{(Raeghi et al. 2011 and 0\% by Sharif et al. 2007) India}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{2.4\% (Sharma et al 2008) Brazil 1.03\% (Pita Gondim et}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{al. 1999), USA 3.2\% (Dubey 1985) and Malaysia 6.3\%}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{(Chandrawathani et al 2008). However, a high}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{seroprevalence of T. gondii in cattle has been reported}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{from many countries of the world; 32\% in Sudan (Khalil}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{and Elrayach, 2011); Tenter et al. (2000) in their paper}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{reported 22\% in the Czech Republic, 40\% in Greece,}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{13-43\% in Netherlands, 43\% in Portugal, 40\% in Spain,}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{69\% in France, 92\% in Italy and 66\% in Turkey. Jacek et}\tabcellsep \tabcellsep \tabcellsep \\
\multicolumn{3}{l}{al. 2007 found 53\% seroprevalence in Poland.}\tabcellsep \tabcellsep \tabcellsep \end{longtable} \par
 
\caption{\label{tab_1}Table 1 :}\end{figure}
 			\footnote{© 2014 Global Journals Inc. (US)} 		 		\backmatter  			  				\begin{bibitemlist}{1}
\bibitem[Khalil et al. ()]{b13}\label{b13} 	 		\textit{},  		 			M Khalil 		,  		 			Khali 		,  		 			Elrayah 		,  		 			E Intisar 		.  		2011.  	 
\bibitem[Dubey et al. ()]{b7}\label{b7} 	 		‘Epidemiology and control of neosporosis and Neospora caninum’.  		 			J P Dubey 		,  		 			G Schares 		,  		 			L M Ortega-Mora 		.  	 	 		\textit{Clin Microbiol Rev}  		2007. 20 p. .  	 
\bibitem[Conraths et al. ()]{b4}\label{b4} 	 		\textit{Nachweis von antikorpern gegn Neospora caninum bei kuhen in hessischen betrieben mit abort und fruchtbarkeitsproblemen. Dtsch Tierarztl Wochen scher},  		 			F J Conraths 		,  		 			C Bauer 		,  		 			W Becker 		.  		1996. 103 p. .  	 
\bibitem[Kuruca et al. ()]{b15}\label{b15} 	 		‘Neospora caninum antibodies in dairy cows and domestic dogs from Vojvodina, Serbia’.  		 			L Kuruca 		,  		 			L Spasojevic-Kosic 		,  		 			S Simin 		,  		 			M Savovic 		,  		 			S Laus 		,  		 			V Lalosevic 		.  	 	 		\textit{Parasite}  		2013. 20  (40)  p. .  	 
\bibitem[Rinaldi et al. ()]{b19}\label{b19} 	 		‘Neospora caninum in pastured cattle: determination of climatic, environmental farm management and individual risk factors using remote sensing and geographic information system’.  		 			L Rinaldi 		,  		 			G Fusco 		,  		 			V Musella 		,  		 			V Veneziano 		,  		 			A Guarino 		,  		 			R Taddei 		,  		 			G Cringoli 		.  	 	 		\textit{Vet Parasitol}  		2005. 128 p. .  	 
\bibitem[Gavrea et al. ()]{b9}\label{b9} 	 		‘Neosporosis and infection with N. caninum in animals from’.  		 			R Gavrea 		,  		 			M Leokaditis 		,  		 			B Losson 		,  		 			V Cozma 		.  	 	 		\textit{Romania. Sc Parait. 1}  		2009. 2 p. .  	 
\bibitem[Moore ()]{b16}\label{b16} 	 		‘Neosporosis in South America’.  		 			D P Moore 		.  	 	 		\textit{Vet Parasitol}  		2005. 127 p. .  	 
\bibitem[Dubey and Thulliez ()]{b6}\label{b6} 	 		‘Persistence of tissue cysts in edible tissues of cattle fed Toxoplasma gondii oocysts’.  		 			J P Dubey 		,  		 			P Thulliez 		.  	 	 		\textit{Am J Vet Res}  		1994. 54 p. .  	 
\bibitem[Antony and Williamson ()]{b0}\label{b0} 	 		‘Prevalence of antibodies to Neospora caninum in dogs of rural and urban origin in central New Zealand’.  		 			A Antony 		,  		 			N B Williamson 		.  	 	 		\textit{N.Z.Vet J}  		2003. 51 p. .  	 
\bibitem[Guimaraes et al. ()]{b10}\label{b10} 	 		‘Prevalence of Neospora caninum antibodies and factors associated with their presence in dairy cattle of the north of Parana state’.  		 			J Guimaraes 		,  		 			S Souza 		,  		 			S L P Bergamaschi 		,  		 			D P Gennari 		,  		 			SM 		.  	 	 		\textit{Brazil. Vet Parasitol}  		2004. 124 p. .  	 
\bibitem[Basso et al. ()]{b1}\label{b1} 	 		‘Prevalence of Neospora caninum infection in dogs from beef -cattle farms, dairy farms and from urban areas of Argentina’.  		 			W Basso 		,  		 			M C Venturini 		,  		 			P Moore 		,  		 			M Ranbeau 		,  		 			J M Unzaga 		,  		 			C Campero 		,  		 			D Bacigalupe 		,  		 			J P Dubey 		.  	 	 		\textit{J Parasitol}  		2001. 87  (4)  p. .  	 
\bibitem[Wouda et al. ()]{b25}\label{b25} 	 		‘Seroepidmio logical evidence for a relationship between Neospora caninum infection in dogs and cattle’.  		 			W Wouda 		,  		 			T Dijkstra 		,  		 			A M H Kramer 		,  		 			C Van Mannen 		,  		 			J M A Brinkhop 		.  	 	 		\textit{Int. J Parasitol}  		1999. 29 p. .  	 
\bibitem[Dubey ()]{b5}\label{b5} 	 		‘Serological prevalence of toxoplasmosis in cattle, sheep, pigs, goats, bison and elk in Montana’.  		 			G Dubey 		,  		 			JP 		.  	 	 		\textit{J Am Vet Med Assoc}  		1985. 186 p. .  	 
\bibitem[Pita Gondim et al. ()]{b17}\label{b17} 	 		‘Serological survey of antibodies to Toxoplasma gondii in goats, sheep, cattle and water buffaloes in Bahia State, Brazil’.  		 			L F Pita Gondim 		,  		 			H V BarbosaJr 		,  		 			C H A Ribeiro Filho 		,  		 			H Saeki 		.  	 	 		\textit{Vet Parasitol}  		1999. 82 p. .  	 
\bibitem[Sharma et al. ()]{b20}\label{b20} 	 		‘Serological survey of antibodies to Toxoplasma gondii in sheep’.  		 			S Sharma 		,  		 			K S Sandhu 		,  		 			M S Bal 		,  		 			H Kumar 		,  		 			S Verma 		,  		 			J P Dubey 		.  	 	 		\textit{J Parasitol}  		2008. 94  (5)  p. .  	 
\bibitem[Vanleeuwen et al. ()]{b23}\label{b23} 	 		‘Seroprevalence of antibodies against bovine leukemia virus, bovine viral diarrhea virus, Mycobacterium avian subspecies paratuberculosis and Neospora caninum in beef and dairy cattle in Manitoba’.  		 			J A Vanleeuwen 		,  		 			A Tiwari 		,  		 			J C Plaizier 		,  		 			T L Whiting 		.  	 	 		\textit{Can Vet J}  		2006. 47 p. .  	 
\bibitem[Vural et al. ()]{b24}\label{b24} 	 		\textit{Seroprevalence of N. caninum in daity cattle herd in central Avatolia, Turkey. Vet archive},  		 			G Vural 		,  		 			E Aksoy 		,  		 			M Bozkir 		,  		 			U Kucukayan 		,  		 			A Erturk 		.  		2006. 76 p. .  	 
\bibitem[Kacar et al. ()]{b12}\label{b12} 	 		‘Seroprevalence of Neospora caninum in cows with abortion history and in dogs sharing the same area in the Kars region’.  		 			C Kacar 		,  		 			H I Gokce 		,  		 			A Akca 		,  		 			O Gungor 		,  		 			S Kaya 		.  	 	 		\textit{Turkey. Revue Med Vet}  		2012. 163  (7)  p. .  	 
\bibitem[Raeghi et al. ()]{b18}\label{b18} 	 		‘Seroprevalence of T .gondii in sheep, cattle and horses in Urmia North-West of Iran’.  		 			S Raeghi 		,  		 			A Akaberi 		,  		 			S Sedeghi 		.  	 	 		\textit{Iranian J Parasitol}  		2011. 694 p. .  	 
\bibitem[Chandrawathani et al. ()]{b2}\label{b2} 	 		‘Seroprevalence of Toxoplasma gondii antibodies in pigs, goats, cattle, dogs and cats in peninsular Malaysia’.  		 			P Chandrawathani 		,  		 			R Nurulaini 		,  		 			C M Zanin 		,  		 			B Premaalatha 		,  		 			M Adnan 		,  		 			Jamnah 		,  		 			S K Khor 		,  		 			S Khadijah 		,  		 			S Z Lai 		,  		 			M A B Shaik 		,  		 			T C Seah 		,  		 			SA 		.  	 	 		\textit{Trop. Biomed}  		2008. 25  (3)  p. .  	 
\bibitem[Jacek et al. ()]{b11}\label{b11} 	 		‘Seroprevalence of Toxoplasma gondii in farm and wild animals from the area of Lublin province’.  		 			S Jacek 		,  		 			Z Jacek 		,  		 			D Jacek 		.  	 	 		\textit{Bull Vet Inst Pulawy}  		2007. 51 p. .  	 
\bibitem[Chikweto et al. ()]{b3}\label{b3} 	 		‘Seroprevalence of Toxoplasma gondii in pigs, sheep, goats and cattle from Grenada and Carriacou, West Indies’.  		 			A Chikweto 		,  		 			S Kumthekar 		,  		 			K Tiwari 		,  		 			B Nyack 		,  		 			M S Deokar 		,  		 			G Stratton 		,  		 			C N L Macpherson 		,  		 			R N Sharma 		,  		 			J P Dubey 		.  	 	 		\textit{J. Parasitol}  		2011. 97  (5)  p. .  	 
\bibitem[Seroprevalence of Toxoplasma gongii antibodies in farm animals (camels, cattle and sheep) in Sudan J medicine and Animal Health]{b14}\label{b14} 	 		‘Seroprevalence of Toxoplasma gongii antibodies in farm animals (camels, cattle and sheep) in Sudan’.  	 	 		\textit{J medicine and Animal Health}  		3  (3)  p. .  	 
\bibitem[Sharif et al. ()]{b21}\label{b21} 	 		‘Serprevalence of Toxoplasma gondii in cattle, sheep and goats slaughtered for food in Mazandaran province, Iran during’.  		 			M Sharif 		,  		 			Sh Gholami 		,  		 			H Ziaei 		,  		 			A Daryani 		,  		 			B Laktarashi 		,  		 			S P Ziapour 		,  		 			A Rafiei 		,  		 			M Vahedi 		.  	 	 		\textit{The Vet Journal}  		2007. 2005. 174 p. .  	 
\bibitem[Tenter et al. ()]{b22}\label{b22} 	 		‘Toxoplama gondii from animals to humans’.  		 			A M Tenter 		,  		 			A R Heckeroth 		,  		 			L M Weiss 		.  	 	 		\textit{Int J Parasitol}  		2000. 30 p. .  	 
\bibitem[Dubey ()]{b8}\label{b8} 	 		\textit{Toxoplasmosis of animals and humans},  		 			J P Dubey 		.  		2010. Boca Raton, Florida: CRC Press. 2.  		 			Taylor and Francis group 		 	 
\end{bibitemlist}
 			 		 	 
\end{document}
