# I. Introduction espite many years of research, mastitis subclinical remains the most economically damaging and zoonotic potential disease for dairy industry and consumers worldwide irrespective of species of animal (Ojo et al., 2009). Economic losses caused by mastitis include value of discarded milk (Radostits et al., 2007). Bacterial contamination of milk from affected cows may render unsuitable for human consumption by causing food poisoning or interference with manufacturing process or in rare cases provides mechanism of spread of disease to humans. Zoonotic diseases potentially transmitted by raw cow milk include brucellosis, caseous lymphadenitis, leptospirosis, listeriosis, melioidosis, Q-Fever, Staphylococcal food poisoning, toxoplasmosis and tuberculosis (Mungube et al., 2005;Radostits et al., 2007). The prevalence of subclinical mastitis in dairy herds is often surprising to producers, moreover, sub-clinically infected udder quarters can develop clinical mastitis and the rate of new infections can be high (Zdunczyk et al., 2003). Previous studies conducted in different countries indicate the distribution and economic importance of the disease. Contreras et al. (1997) Subclinical mastitis can be recognized indirectly by several diagnostic method including the California mastitis test (CMT), the Modified White Side test, Somatic cell count, pH, and catalase tests. These tests are preferred to be screening tests for subclinical mastitis as they can be used easily, yielding rapid as well as satisfied results (Joshi and Gokhale, 2006). In some parts of Ethiopia, the disease is insufficiently investigated and information relating to its magnitude, distribution and risk factors is scant. Such information is important to envisage when designing appropriate strategies that would help to reduce its prevalence and effects (Mekebib et al., 2009;Megersa et al., 2010). This study aimed (i) to evaluate the prevalence of subclinical mastitis in apparently healthy dairy cows in Holeta district, (ii) to determine the most frequency of intramammary infection, causative agents, and (iii) to evaluate associated risk factors affecting on subclinical mastitis. # II. Materials and Methods # a) Study area The study was conducted in Addis Ababa city administration, the capital of the Federal Democratic Republic of Ethiopia. The city covers an area of 530.14 km 2 and is sub divided into ten sub-cites namely, Arada, Bole, Addis Ketema, Nefas Silk Lafto, Kolfe Keranio, Akaki Kality, Yeka, Lideta, Kirkos and Gulele sub-cites. Addis Ababa lies at an altitude of 2000-3000 meters above sea level and is a grass land biome located between 9.03 North latitude and 38.74 East longitudes. The city has alternating dry and rainy seasons with the long rainy season that extends from June to September and short rainy season that lasts from March to May. The mean annual minimum and maximum temperatures range between 14 o C and 21 o C respectively with an overall overage of 17 o C. The mean relative humidity is 61.3% (CSA, 2003). # b) Study Animals and Sample Size Determination The study was conducted on 444 lactating cows (local, Holstein-Friesian, Jersey and cross breeds) from 37 dairy farms in Addis Ababa. The farms were purposively selected based on the availability of lactating cows within the farm and the owners' willingness. Systematic random sampling method was applied for the selection of individual animals (lactating cows) in the farms. The sample size was determined by the formula given by Thrusfield (2007) considering an expected prevalence of 71% (Mekibib et al., 2009), 95% confidence level and 5% desired precision. Adding a few more samples to improve on the accuracy, a total of 444 lactating cows were considered for the study. # c) Study Design A Cross sectional study was conducted. Three dairy farms were purposively selected for their ease accessibility. Simple random sampling technique was followed to select the study animal and the desire sample size was calculated according to the formula given by Thrusfield (2007). The study was carried out from November 2011 to April 2012 by collection of events associated with mastitis in lactating cows from 37 small holder's dairy farms in Addis Ababa. # d) Study Methodology i. Clinical inspection of udder The udder was first examined visually and then by palpation to detect possible fibrosis, inflammatory swellings, visible injury, tick infestation, atrophy of the tissue and swellings of supra mammary lymph nodes. The teat condition (color changes, swelling at or near the teat base, swelling or firmness at or near the teat end, openness of the teat orifice, teat skin condition, signs of vascular damage like petechial hemorrhage, etc.) was evaluated during clinical examination (More, 1989). Upon palpation, one can feel hot, painful swelling on udder and ventral abdomen and was manifested by loss of appetite, depression, recumbence and blood mixed milk in acute mastitis. In chronic mastitis, continuous or intermittent discharge of pus, clots, flakes or watery secretion will be seen from the udder (Chauhan and Agarwal, 2006). # e) California mastitis test (CMT) The California Mastitis Test (CMT) was performed according to the manufacturer's instruction. In brief, a small sample of milk (approximately ½ teaspoon) was collected from each quarter into a plastic paddle that has 4 shallow cups marked A, B, C and D. An equal amount of CMT reagent was added to the milk and the paddle rotated to mix the contents. After approximately 10 seconds, the score was read while continuing to rotate the paddle. Results were recorded as T (trace), 1, 2 or 3 based on the level of precipitation (coagulation) (Mellenberger and Carol, 2000). i. Risk factor assessment Information on animal and farm-based risk factors was collected in two separate pre-designed questionnaires, by observation, and by interviewing of the different farm attendants and owners. A check-list was used to record such information as the cows' age, breed, parity, lactation stage, and body condition, problems of leaking milk and previous history of mastitis. Farm-based risk factors considered were teat drying, teat cleaning, floor types, teat dipping, milkers, bedding and treatment history. ii. Assessment of public health risks This was done by asking respondents weather they adapt the behavior of boiling milk before consumption, stripping of the foremilk at the start of milking, and by asking them the time duration of time they withheld milk before distribution to the public if the animals were treated for mastitis. # f) Statistical analysis The data was compiled and analyzed with SPSS statistical package version 17. Prevalence estimation of commonly isolated pathogens in Holeta town dairy farms was determined using standard formulae (i.e., the number of positive animals/samples divided by the total number of animals/samples examined). Descriptive statistics such as percentages and frequency distributions was used to describe/present the nature and the characteristics of the data. # III. Results # a) Prevalence of Mastitis at Individual Cow and Quarter Level Three hundred forty three Holstein-Friesian (HF), 20 Jersey, 15 local and 32 cross (HF X Local) breeds were included in the study. Of the total 444 lactating cows, 302 (68%) were found to be affected with clinical or sub clinical mastitis based on clinical examination of the udder and CMT results. From these, 94 (21.2%) was clinical and 208 (46.8%) was sub-clinical mastitis (Table 1 Breed, age, parity and lactation stages have significant influence (P<0.05) on the prevalence of bovine mastitis. There was a significant difference in prevalence between animals of different age categories (P<0.05). The highest prevalence (86.5%) was found in lactating cows of ages 7-10 years, followed by cows of ages 11-13 years (81.8%), and the lowest prevalence (59.1%) was recorded in cows of ages 3-6 years. Higher prevalence (90.8%) was recorded in cows which gave birth to 4-7 calves and the lower prevalence (61.6%) was recorded in cows that gave birth to 1-3 calves. The difference was statistically significant (P<0.05) (Table 3). The effect of lactation stage on the current prevalence of mastitis was studied and analyzed and the result revealed that lactation stage had significant effect (P<0.05) on the prevalence of mastitis. Higher prevalence (89.3%) of mastitis was observed and recorded in cows of late lactation stage (9-14 month) followed by cows in mid (83.65%) lactation (5-8 month) and early lactation stage (3 week-4 month) that had a prevalence of 50.7%. The effect of breed on the prevalence of mastitis was also studied and analyzed and the result revealed that breed had significant effect (P<0.05). Among the different breeds studied, the highest mastitis prevalence was observed in Holstein-Friesian breeds (71.8%) followed by Jersey (70.0%), local (66.7%), and cross (48.5%) breeds (Table 3). Questionnaires were distributed to 24 farms among the 37 farms included in the study. One questionnaire per farm owner/attendant was distributed. The entire farms included in the study followed manual milking (hand milking) system and most (80%) of the milkers were males. No specific sequence is followed during milking in many (87.5%) of the farms. Rather, it depends on the placement of the animal in the shed. Fifty four percent of the farm owners were educated to high school level while 12.5% were educated up to university level. The remaining (33.5%) attended elementary schools. Overall, educated people had better know how about the zoonotic implications of consuming raw milk, predisposing factors for mastitis and drug residue effect post treatment of mastitic animals. A few (12.5%) farmers emphasized the need to milk healthy cows first and the diseased cows later to prevent transmission of disease. Most (66.7%) of the milkers used disinfectant before milking only while 8 (33.3%) milkers said that they use disinfectant both before and after milking. Tap water is the primary source of water to clean teats and hands in many (91.7%) of the farms while few (8.3%) milkers use river water for teats and hands cleansing. Eighteen (75%) farms strip the foremilk first while few undertake direct milking to the material used for milking. Among the 24 farms, 8 (33.3%) used individual towels, 10 (41.7%) communal towel and 6 (25%) did not use towel for drying of teats before or after milking. Among the 24 farms, 14 (58.3%) milkers disinfect their hands before proceeding to milk the next cow while 10 (41.4%) milkers disinfect their hands only at the beginning of milking. Most (75%) of the farmers boil milk before consumption while few (25%) milkers consume raw milk. In almost all of the farms, `animals were previously treated for mastitis while few animals (heifers that gave the first and second calf) were not treated for mastitis cases. Few (20.8%) farms distribute the milk for public consumption starting from the same day the animals were treated while most (79.2%) withhold the milk depending on the withdrawal period of the drug as prescribed by veterinarians. The management (housing, bedding, feeding, etc.) and the degree of sanitation were also observed. Among the 24 farms, there were leakage of urine, feces and milk during milking in 7 (29.2%) while in the remaining (14 farms), the bedding, housing and other degree of sanitary measures like milking procedures, use of disinfectant etc. were good. # IV. Discussion A total of 444 dairy cows, from which 343 HF, 20 Jersey, 66 cross (HF x local), and 15 local breeds from Addis Ababa were investigated in a cross sectional study conducted between November 2011 and April 2012. The current prevalence of mastitis was 68.0%. The finding in this study is greater than that of , who reported 44.1% and Nibret et al. (2011), who reported 32.6% in different parts of Ethiopia. The high prevalence of sub-clinical mastitis may be attributed to improper milking hygiene, lack of post milking teat dipping and contact labors used, absence of order in milking cows of different ages and milking of mastitic animals before the healthy ones all of which might have increased the prevalence (Radostits et al., 2007). The quarter level prevalence was 41.9% (744/1776). This finding was greater than that of Benta et al. (2011), who reported 31.4% (349/1112). This difference in the observed prevalence of mastitis among studies may be attributed to various factors like management, environmental, animal risk factors and causative agents (Radostits et al., 2007). This study revealed a higher prevalence of subclinical mastitis (46.8%) (Abdelrahim et al., 1989). This may be attributed to the difficulty of detecting sub-clinical mastitis by the owners compared to the easily detectable clinical cases which prompt owners seek treatment for their animals (Radostits et al., 2007). Increasing age, lactation stage, parity and poor management increased the risk of mastitis. This is line with previous reports on mastitis in Ethiopia (Kerro Dego and Tareke, 2003) and industrialized countries (Schukken et al., 1989). Stage of lactation was a risk factor for mastitis (Mungube et al., 2004). In late lactation the risk of mastitis increased. Two reports on Ethiopian conditions found higher prevalence of mastitis during early lactation than late lactation (Hussien, 1999). The reason may be due to excluding of lactating cows below 3 weeks to avoid false positive result since SCC increases during early lactation (Tesfu et al., 1999). Manual milking methods in the entire farms that included in this study was the major predisposing factors to increase the prevalence of mastitis. Most of employed milkers have little educational background and have limited knowledge about the mechanism/s of disease transmission. Often, they do not disinfect their hands and teats during and between milking of different cows, use of communal towel for drying of teats and also, they have no special preference between tape and river water. This study also noted a high prevalence of mastitis in farms that use river water for sanitation. Sequence of milking cows also seemed to have a role on the prevalence of mastitis. For example, in farm A which employ a specific sequence (first milk healthy heifers, healthy cows and last diseased cows), the prevalence was lower as compared to the other farms in which they apply random milking procedures in the placement of cows in the shed. In this study, 33.3% of the farm attendants reported to consume raw milk. This practice can be said as risky as raw milk can contain a variety of diseasecausing pathogens, as demonstrated by numerous scientific studies. These studies, along with numerous milk borne out breaks, clearly demonstrated the risk associated with drinking raw milk. For instance, in the US alone, there were 85 reported outbreaks of human infections over the years 1998-2008 due to the consumption of contaminated milk, 1614 illness, 187 hospitalizations and two deaths (Thorne, 2011). There is also concern that small amounts of certain antimicrobial agents (residue) may significantly shift the resistance patterns in the microbial population in human intestinal tract, allergy from residue of penicillin etc. (Jones, 1999). The present study also found reluctance in 79.2% of the farm owner's to withhold milk from mastitic cows after treatment. Pasteurization effectively kills raw milk pathogens without any significant impact on milk nutritional quality. Stripping of the foremilk is also necessary as it contains many microbes that affect human health negatively. # V. Conclusion and Recommendations In a spite of a large research efforts aimed to gain prevalence and to develop a new control tools for mastitis, the subclinical occurrence of the mastitis remains a substantial problem for dairy producers. The result of the present study indicated a relatively high prevalence of subclinical mastitis in dairy cattle of the study area. The relatively high prevalence reported in this study was clearly indicated lack of strategic control measures against the disease as well as poor surveillance measures. Lack of maintenance of strict hygiene and good sanitary environment may be contributory factors in the cause of subclinical mastitis. It is therefore important that farmers should ensure strict personal hygiene and that of animals and general sanitary condition of the farms should be improved and maintained. Furthermore, all dairy producers know that early detection of intramammary infection is important for selecting and implementing proper therapy. Unfortunately, most infections are not detected until they become clinical, and by then extensive and costly damage can result. Routine milk cultures should be an ongoing part of any mastitis control program. The sampling strategies for any ongoing program require the input of the herd veterinarian as well as herd management. 1Types of mastitisTotal number examinedPositive (%)? 2P-ValueClinical44494 (21.20)Subclinical444208 (46.80)52.0780.000Total444302 (68.00)b) Prevalence of Bovine Mastitis across DifferentCategories of Cows 3VariablesNo. examinedPositive (%)?2P-ValueBreedHolstein-Friesian (HF)343246 (71.7)Jersey2014 (70.0)13.786.003Cross (local x HF)6632 (48.5)Local1510 (66.7)Total444302 (68)Age3-6 years296175 (59.1)7-10 years126109 (86.5)32.497.00011-13 years2218 (81.8)Total444302 (68)Parity1-3346213 (61.6)30.048.0004-79889 (90.8)Total444302 (68)Lactation stage3 week-4 month223113 (50.7)5-8 month146122 (83.65)62.722.0009-14 month7567 (89.3)Total444302 (68)c) Questionnaires Survey, Observation and Interviewing ## Conflict of Interest The authors have no declared any conflict of interest ## VI. Acknowledgements Authors would like to thank Aklilu Lemma Institute of Patho-biology for their material and technical supports. * Prevalence of mastitis in imported Friesian cows in Sudan AIAbdelrahim AMShommein HBSuliman SA IShaddad Revue Elev. Med. Vet. Pays Trop 42 1989 * Observation on the prevalence of caprine mastitis in relation to predisposing factors in Maiduguri JAAmeh LSTari Small Ruminant Res 35 2000 * Prevalence and distribution of mastitis pathogens and their resistance against antimicrobial agents in dairy cows Jordan DAzmi FHawari AIDabbas American Journal of Animal and veterinary sciences 3 1 2008 * Dairy management practices associated with incidence rate of clinical mastitis in low somatic cell score herds in France JBarnouin SBord SBazin MChassagne Journal of Dairy Sci 88 2005 * Mastitis in lactating camels (Camels dromedarus) in Afar Region TBekele BMolla Northeast Ethiopia. Berl. Munch Tieriaztl Wochenschr 114 2001 * Study on Prevalence of Mastitis and its Associated Risk factors in Lactating Dairy cows in Batu and its Environs DBBenta TMHabtamu Ethiopia. Global veterinarian 7 6 2011 * Prevalence and Risk factors of mastitis in lactating dry cows in southern Ethiopia DBiffa EDebela FBeyene Inter. J. of Applied Research in Veterinary Medicine 3 3 2005 * Study on bovine mastitis in dairy farms of Bahir Dar town and its environ MBitew ATafere TTolosa J. Anim. Vet. Adv 9 2010 * Central statistical Authority (CSA) Ethiopia Addis Ababa 2003 1 Ethiopia Livestock Estimate * Textbook of Veterinary Clinical and Laboratory Diagnosis. 2 n ed RSChauhan DKAgarwal 2006 Jaypee Brothers Medical Publishers 103 New Delhi * Persistence of Caprine intramammary pathogens throughout lactation AContreras JCCorrales ASanchez DSierra J. of Dairy Sci 80 1997 * Effect of mastitis and related germ yield and composition during naturally occurring udder infections in dairy cows JBCoulon PGasqui JBarnoun AOllier PPardel DominiqueP Anim. Res 51 2002 * Ethiopian Agricultural Sample Enumeration (EASE) CSA 2010 * Livestock Sector, Brief Livestock Information, Sector Analysis and Policy Branch Fao 2003 Rome, Italy * Survey on the prevalence of bovine mastitis and the predominant causative agents in Chaffa valley KFekadu Proceedings of the 9 th Conference of Ethiopian Veterinary Association the 9 th Conference of Ethiopian Veterinary AssociationAddis Ababa, Ethiopia 1995 * Milk yield and associated economic losses in quarters with subclinical mastitis due to Staphylococcus aureus in Ethiopian crossbred dairy cows YTGebreyohannes FGRegassa BKelay Trop. Anim. Health Prod 42 2010 * Study on prevalence of mastitis on cross breed Dairy cow around Holeta areas. Global veterinaria DDGirma 2010 5 * Study on Prevalence of Bovine Mastitis on cross Breed Dairy cows Aroun Holeta Areas, West Shewa Zone of Oromia DDGirma Ethiopia. Global Veterinaria 5 6 2010 * Prevalence of mastitis at Alemaya University dairy farm TGirma Journal Ethiopian Veterinary 2001 * Physiology of mastitis and factors affecting somatic cell counts RJHarmon J. Dairy Sci 77 2103 1994 * Laboratory and field handbook on bovine mastitis JSHogan RNGonzalez RJHarmon SCNickerson SPOliver JWPankey KLSmith National Mastitis Council 12 1999 * SJHogan RNGonzalez JRHarmon SCNickerson SPOliver JWPankey LKSmith 1999 * Laboratory Handbook on Bovine Mastitis. Published by National Mastitis Council, Inc W D Hoard * Cross-sectional and longitudinal study of bovine mastitis in urban peri-urban dairy systems in the Addis Ababa region NHussein 1999 Ethiopia; Ethiopia Free University of Berlin, Germany and Addis Ababa University MSc thesis Joint programme * Bacteriology of mastitic milk and in vitro antibiogram of the isolates. Pak MIqbal MAKhan BDaraz USaddique Vet. J 24 2004 * Professor and Extension Dairy Scientist, Milk Quality and Milking Management, Department of Dairy Science, Virginia Tech GMJones 1999 * On-farm Tests for Drug Residues in Milk. The cattle site Ltd * Status of mastitis as an emerging disease in improved and periurban dairy farms in India SJoshi SGokhale Ann. New York Acad. Sci 1081 2006 * The Merck Veterinary Manual. 9 th edition CMKahn 2005 Merck and Co., Inc. Whithhouse Station, N. J U.S.A * Clinical and subclinical mastitis in smallholder dairy farms in Tanzania. Risk intervention and knowledge transfer EDKarimuribo JLFitzpatrick CEBell ESSwai DMKambarage NHOgden MJBryant NPFrench Prev. Vet. Med 74 2006 * Bovine mastitis in selected areas of southern Ethiopia. Tropical Animal Health and Production OKerro Dego FTareke 2003 35 * Relationships between the results of mastitis tests, somatic cell counts and the detection of mastitis agents in milk LMKozacinski TIladziosmanovi IKMajic Jole ZCvrtila Paraxis Vet 57 2002 * Prevalence and major bacterial causes of bovine mastitis in Asella, South Eastern Ethiopia MLakew TTolosa WTigre Trop. Anim. Hlth. Production 41 2009 * Occurrence of mastitis and associated risk factors in lactating goats under pastoral management in Borana, Southern Ethiopia BMegersa TChala FAbunna ARegassa MBerhanu DEtana Trop. Anim. Hlth. Production 42 2010 * Bovine mastitis prevalence, risk factors and major pathogens in dairy farms of Holeta Town, central Ethiopia BMekebib MFurgasa FAbunna BMegersa AFurgasa Veterinary World 13 9 2009 * Bovine Mastitis: Prevalence, Risk Factors and Major Pathogens in Dairy Farms of Holeta Town, central BMekibib MFurgasa FAbunna BMegersa ARegassa Ethiopia. Veterinary World 3 9 2009 * RMellenberger Dept Animal Sciences April, 2000 Michigan State University and Carol, J. Roth, Dept. of Dairy Science, University of Wisconsin-Madison * Bacterial causes of mastitis in south Ethiopia, annual research report AMohammed DEtana 1996 Hawasa college of agriculture * Tick borne Diseases of Livestock in Africa PCMore Manual of Tropical Veterinary Parasitology. English version. C. A. B. International: Wallingford 331 1989 * Prevalence of mastitis in dairy goats in some selected farms in Morgoro and Arusha NGMoshi GCKitaro UMMinga Tanzania. Tanzania J. Agric. Sci 1 1998 * Reduced milk production in udder quarters with subclinical mastitis and associated economic losses in crossbred dairy cows in Ethiopia EDMungube BATenghagen FRegassa MNKyule YShiferaw TKassa MpoBaumann Trop. Anim. Hlth. Produ 37 5 2005 * Risk Factors for Dairy Cow Mastitis in the Central Highlands of Ethiopia. Tropical Animal Health and Production EOMungube BATenhagen MNKyule MGreiner MP OBaumann TKassa FRegassa 2004 36 * Microbiological procedures for the diagnosis of udder infection. 3 rd ed 2004 National Mastitis Council Inc Arlington, VA National Mastitis Council * The prevalence of subclinical mastitis in dairy goats in Kenya ENNdegwa CMMulei SJMunyna J. South Afr. Vet. Assoc 71 2000 * Escherichi coli, O157:H7 in Food animals in part of south-western Nigeria. Prevalence and invitro antimicrobial susceptibility OEOjo MAOyekunle AOOgunleye EBOtesile Trop. Vet 26 3 2009 * Antimicrobial resistance in developing countries. Part I: recent trends and current status The Lancet Infectious Diseases INOkeke RLaxminarayan ZABhutta AGDuse PJenkins TFO'brien APablos-Mendez KPKlugman 2005 5 * Clinical Veterinary Microbiology PJQuinn MECarter BMarkey GRCarter 2004 London Wild life Publisher * Mastitis. In Clinical Veterinary Microbiology PJQuinn MECarter BKMarkey GRCarter 1999 Mosby International Limited London * PJQuinn M ECarter BKMarky G RCarter Clinical Veterinary Microbiology 1994 1 ed. London: Wolfe publishing * Veterinary Medicine. A Textbook of the Diseases of Cattle, Sheep, Pigs, Goats and Horses OMRadostits CCGay KWHinchcliff PDConstable 2007 Saunders Elsevier, Spain th edition * A text book of the diseases of cattle, horses, sheep, pigs, and goats OMRadostits 2007 * Incidence rate of clinical mastitis on Canadian dairy farms RgmoRiekkerink HWBarkema DFKelton TScholl J. of Dairy Sci 91 2008 * Detection of inflammatory changes in milk MSandholom The bovine udder and mastitis Finland MSandholom THonkanen_Buzalski LKaartinan Pyorala 1995 * Incidence of clinical mastitis on farms with low somatic cell counts in bulk milk YHSchukken FJGrommers DVan De Geer ABrand Veterinary record 125 1989 * Microbiological results from milk samples obtained pre-milking and post-milking PWSears DJWilson RNGonzalez DDHancock J. of Dairy Sci 74 1991 * Preparation and evaluation of Staphylococcus aureus vaccines for the control of mastitis in dairy buffaloes (Bubalus bubalis). PhD Dissertation, Department of Veterinary Clinical Medicine and Surgery, College of Agriculture AShakoor 2005 Faisalabad, Pakistan * Mastitis control in dairy production ASharif MUmer GMuhammad J. Agri. Soc. Sci 5 2009 * Dairy Cattle Mastitis in and around Sebeta HSori AZerihun SAbdicho Ethiopia. Inter. J. Applied Research in Veterinary Medicine 3 2005 * Prevalence of mastitis at Alemaya University Dairy Farm GTefera J. Eth. Vet. Ass 3 2001 * Cross Border Livestock Trade and Food Security in the Southern and Southeastern Ethiopia Borderlands. OSSREA (Organization for Social Science Research in Eastern and Southern Africa TTegegne AAlemayehu GMAyele Development Research Report Series. No.1. Commercial Printing Enterprise: Addis Ababa 1999 * Survey of Mastitis in Dairy Herds in the Ethiopian Central Highlands. SINET: Ethiop KTesfu WGemechu TAzage J. Sci 22 2 1999 * Side effects of Dairy Raw milk. Proud Partner of the Lance Armstrong Foundation RThorne 2011 8 * Veterinary epidemiology MThrusfield 2007 Blackwell Publishing 3 rd edition * Veterinary Epidemiology. 2 n ed. Black well science Ltd MThrusfield 1995 UK. * Patterns of clinical mastitis in Danish organic dairy herd MVaarst CEnvoldsen J. Dairy Sci 64 23 1997 * Dairy Development in Sub-Saharan Africa: A study of Issues and option MTWalshe TGrindle ANell MBaschaman Africa Technical Development Series 135 1995 World Bank Technical Paper * Macroeconomic, international trade and sectoral policies in livestock development: an analysis with particular reference to low income countries TOWilliams DADerosa OBadiane Livestock development strategies for low income countries. Proceeding of the Joint FAO/IL Roundtable on livestock development strategies for low income countries RTWilson SEtui SMack ILRI, Addis Ababa, Ethiopia 1995. 27 February 02 March * Prevalence and etiology of mastitis in cows from two major Ethiopian dairies SMWorkineh MBayleyegne HMekonnen LndPotgieter J. Trop. Anim. Hlth. Prod 34 2002 * Reproductive management and reproductive performance of dairy herds in urban and peri-urban dairy production systems in Addis Ababa milk shed AYohannes JordanAl-Salt MYoseph TAzage YAlemu NNUmunna 2003. 1998 Ethiopia Al-Salt University College MSc Thesis Clinical and sub-clinical mastitis in primiparous dairy heifers in Jordan. In: proceeding of the 6 th annual conference of ESAP Addis Ababa * Importance of oestrogen and oestrogen active compounds for udder health in cattle. A review SZdunczyk HZerbe MHoedemaker Dtsch Tierarztl Wochenschr 110 461 2003