The Evaluation of the Wines Antioxidant Activity

Abstract

A new method for measuring the antioxidant activity is the method which using N, N' -di ethyl-p-phenylendiamina (DMPD). In this paper, was verified of their effectiveness of the DMPD method on antioxidant foods. We used wine samples coming from different areas of Romania. Antioxidant action of wines is strictly related to the amount of polyphenols. To evaluate the sensitivity of the method, the system was tested by using of standard solution of TROLOX 1 mg/m L and DMPD: Fe Cl 3 molar ratio of 10:1. Spectrofotometric measurements were recorded by using an UV-VIS Jenway 6300 at 505 nm. Antioxidant action was expressed as TEAC (TROLOX equivalent antioxidant capacity), using the calibration curves plated with different amounts of TROLOX. These results show that the red wine samples have a high antioxidant action, in conformed to the amount of polyphones. The method ensures sensibility and reproducibility in the measurement of antioxidant action of hydrolytic compounds. Keyworks : antioxidant activity, polyphones, DMPD method, wines.