A Novel Elicitor (Pi PE) from Phytophthora Infestans Induces Active Oxygen Species and the Hypersensitive Response in Potato

Naotaka Furuichi; Kazutoshi Yokokawa; Hisakazu Okamura; Masahiro Ohta

Authors

Naotaka Furuichi
Kazutoshi Yokokawa
Hisakazu Okamura
Masahiro Ohta

Keywords:

hypersensitive cell death, PiPE elicitor, potato, Phytophthora infestans, signal transduction of HR, fructose 1, 6-bisphosphate aldolase

Abstract

A novel elicitor (Pi PE) from the oomycete Phytophthora infestans (Pi) stimulates the hypersensitive response (HR) in potato. The Pi PE, purified by anion-exchange chromatography from a water-soluble extract of Pi caused cell death, characteristic of HR, and enhanced active oxygen species (AOS) generation in tuber tissues. The partial amino acid sequence, and the sequence of the Pi PE c DNA derived by PCR had homologous domain to fructose 1,6 bisphosphate aldolase (FBA) genes. To demonstrate that the Pi PE c DNA encodes an active elicitor, we expressed Pi PE in Echerichia coli, high five insect cells and purified the recombinant protein. His-Pi PE induced HR, browning and generation of AOS in potato tissues. The Pi PE was produced in the germination fluid from Pi and was existing in the cell wall of Pi. The role of Pi PE peptides in the induction of HR in an incompatible interaction between Pi and potato cells is a prerequisite for the AOS and HR induction.

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A Novel Elicitor (Pi PE) from Phytophthora Infestans Induces Active Oxygen Species and the Hypersensitive Response in Potato. (2013). Global Journal of Medical Research, 13(16), 1-14. https://medicalresearchjournal.org/index.php/GJMR/article/view/

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References

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