DNA Normality Following in Vitro Sperm Preparation with Pentoxifylline and L-Carnitine for Asthenozoospermic Infertile Men

Saad S. Al-Dujaily; Yahya K. Al-Sultani; Nawras N. Shams Alddin

Authors

Saad S. Al-Dujaily
Yahya K. Al-Sultani
Nawras N. Shams Alddin

Keywords:

pentoxifylline, l-carnitine, in vitro activation, layering preparation technique, male infertility

Abstract

Background: Sperm cells from infertile patients have poor motility and defected DNA. Pentoxifylline (PX) and L- carnitine (LC) are added in the medium for in vitro activation to increase sperm motility and improve the pregnancy outcome. However, there are few studies about the effect of PX with LC medium on sperm DNA normality. Objective: The goal of this study is to found out the optimum medium can increase the active motility percentage and reduce sperm DNA damage for asthenozoospermic men using the motility stimulant substances, PX and LC for this purpose. Methods: Semen was collected from 100 infertile men involved in the current study. Each semen sample was divided into four portions. One part was considered as a control group and in vitro activated by using culture medium only. The other portions were considered as treated groups and in vitro activated by adding PX (1mg) and/or LC (0.5mg) to the culture medium. Certain sperm function parameters were examined before and following in vitro activation using layering technique. Sperms DNA damage was detected by using acridine orange (AO) test. Results: The results revealed a highly significant (P

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DNA Normality Following in Vitro Sperm Preparation with Pentoxifylline and L-Carnitine for Asthenozoospermic Infertile Men. (2013). Global Journal of Medical Research, 13(26), 25-30. https://medicalresearchjournal.org/index.php/GJMR/article/view/

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Author Biography

Saad S. Al-Dujaily

Dr. Saad S. Al-Dujaily is a distinguished researcher and academic leader in the field of infertility and assisted reproductive technologies. He serves as Vice Dean for Scientific Affairs and Postgraduate Studies at the High Institute for Infertility Diagnosis and ART, Al-Nahrain University, Baghdad, Iraq. With 59 publications and 166 citations, his work focuses on sperm preparation techniques, including the use of progesterone, pentoxifylline, and herbal extracts for asthenozoospermic men. Dr. Al-Dujaily is also the General Secretary of the Iraqi Fertility Society. His research has contributed to understanding DNA normality and improving IUI outcomes. He is indexed in Scopus and OpenAlex, and holds an ORCID iD.

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