Effects of Adding Human Follicular Fluid and Pentoxifylline on IUI Outcome of Asthenozoospermic Patients

Saad S. Al-Dujaily

Authors

Saad S. Al-Dujaily

Keywords:

Abstract

Background: Although there are many motility stimulants have been found to play an important role in improving the active sperm motility and morphologically normal sperm (MNS) percentages when added in vitro with the culture medium for semen ,the results of pregnancy rate following intra-uterine insemination (IUI)still not exceed 20% . Objective: This study is a trial to improve the results of pregnancy rate following IUI by adding the follicular fluid (FF),and pentoxifylline (PX) to the culture medium used for preparation of asthenozoospermic semen samples. Subjects, Material and methods: Ninety five infertile couples were involved in the current study (asthenozoospermic male partners with intact spouses) divided into three groups according to the sperm stimulants which were used for in vitro sperm activation followed by intrauterine insemination technique. Group one was FF-treated semen samples and consists of 40 couples, group two was FF+PX-treated semen samples and consists of 25 couples and lastly, group three was Ham's F-12 culture medium as a control group and consists of 30 couples. All the couples were followed up for determining outcome of pregnancy after IUI was done. Results: Out of the all 95 couples, 22 pregnancies (23.15%) were resulted following IUI. The best results were obtained by group one (FF treated group) in which out of 40 cases enrolled in this group, 12 pregnancies (30%) were resulted following IUI. Regarding group two (FF+PX treated group), out of 25 cases enrolled in this group, 5 women (20%) were pregnant following IUI. Regarding group three (Ham's F-12 treated group), out of 30 cases enrolled in this group, 5 women (16.67%) were pregnant following IUI. Conclusion: Adding FF to culture media for in vitro sperm activation was associated with a higher pregnancy rate following IUI than other treated culture media.

Downloads

How to Cite

Effects of Adding Human Follicular Fluid and Pentoxifylline on IUI Outcome of Asthenozoospermic Patients. (2014). Global Journal of Medical Research, 14(33), 13-17. https://medicalresearchjournal.org/index.php/GJMR/article/view/

Download Citation

Author Biography

Saad S. Al-Dujaily

Dr. Saad S. Al-Dujaily is a distinguished researcher and academic leader in the field of infertility and assisted reproductive technologies. He serves as Vice Dean for Scientific Affairs and Postgraduate Studies at the High Institute for Infertility Diagnosis and ART, Al-Nahrain University, Baghdad, Iraq. With 59 publications and 166 citations, his work focuses on sperm preparation techniques, including the use of progesterone, pentoxifylline, and herbal extracts for asthenozoospermic men. Dr. Al-Dujaily is also the General Secretary of the Iraqi Fertility Society. His research has contributed to understanding DNA normality and improving IUI outcomes. He is indexed in Scopus and OpenAlex, and holds an ORCID iD.

References

(2010) Laboratory manual for the examination and processing of human semen.

Gert Dohle (2010) Male infertility in cancer patients: Review of the literature.Int.J.Urol.2010. 17(4), 327-331. https://doi.org/10.1111/j.1442-2042.2010.02484.x

Aitken RJ, Baker MA, Nixon B (2010) New insights into sperm physiology and pathology. 99-115.

A. Steiber, J. Kemer, C. Hoppelm (2004) Carnitine: a nutritional, biosynthetic and functional perspective. 25(5-6), 455-73.

Revelli LD, Casano S, Molinaril E, Massobrio M, Rinaudo P (2009) Follicular fluid content and oocyte quality: from single biochemical markers to metabolomics. 7(1), 7-40. https://doi.org/10.1186/1477-7827-7-40

Leroy JL, Vanholder T, Delanghe JR (2004) Metabolic changes in follicular fluid of the dominant follicle in high-yielding dairy cows early post partum. 62(6), 1131-1143. https://doi.org/10.1016/j.theriogenology.2003.12.017

Wen X, Li D, Tozer AJ (2010) Estradiol, progesterone, testosterone profiles in human follicular fluid and cultured granulosa cells from luteinized preovulatory follicles. 8(1), 117. https://doi.org/10.1186/1477-7827-8-117

Guidobaldi HA, Teves ME, Unates DR (2008) Progesterone from the Cumulus Cells Is the Sperm Chemoattractant Secreted by the Rabbit Oocyte Cumulus Complex. 3, 30-40.

H. Abdelrazik, A. Agrawal (2009) L-carnitine and assisted reproduction. 5, 43-47.

Bjorndahl L, Mohammadieh M, Pourian M (2005) Contamination by Seminal Plasma Factors During Sperm Selection. 2, 170-173.

S. Verhulst, B. Cohlen, E. Hughes, M. Heineman, E. Te Velde (2006) Intra-uterine insemination for unexplained subfertility.

Casadei L, Zamaro V, Calcagni M (2006) Homologous intrauterine insemination in controlled ovarian hyperstimulation cycles: A comparison among three different regimens European J. 129, 155-161.

Andersen CY, Humaidan P, Bredkjær H (2006) Hormonal characteristics of follicular fluid from women receiving either GnRH agonist or hCG for ovulation induction Hum Reprod. 21(8), 2126-2130. https://doi.org/10.1093/humrep/del119

Bedaiwy M (2012) Effect of follicular fluid oxidative stress parameters on intracytoplasmic sperm injection outcome Gyne Endocr. 28(1), 51-55. https://doi.org/10.3109/09513590.2011.579652

Allen VM, R. Wilson (2006) Pregnancy outcomes after assisted reproductive technology J Obst Gyne. 28(3), 220-233.

Merviel P, Heraud M, Grenier N (2008) Predictive factors for pregnancy after intrauterine insemination. 10, 120-129.

Dickey RP, Brinsden PR, Pyrzak R (2010) Insemination technique and insemination complications. 109-118.

T. Glover, K. Mitchel (2008) Introduction to Biostatistic. 4.

Cooper TG (2010) Physiology of Sperm Maturation and Fertilization. 61-86.

Bahat A, Eisenbach M (2006) Sperm thermotaxis. 252(1-2), 115-119.

Michael Eisenbach, Laura Giojalas (2006) Sperm guidance in mammals - an unpaved road to the egg Nat Rev Mol Cell Biol. 7(4), 276-285. https://doi.org/10.1038/nrm1893

G. Machado, L. Lefievre, C. Ford (2008) Mobilisation of Ca2+ stores and flagellar regulation in human sperm by S-nitrosylation: a role for NO synthesised in the female reproductive tract Dev J. 135(22), 3677-3686.

Gregory Michael A, Piercy E (1995) Ovarian 11 beta hydroxysteroid dehydrogenase activity is inversely related to the outcome of in vitro fertilization-embryo transfer treatment cycles. 64(3), 590-598.

Koli P, Anil M, Patil K (2013) Intrauterine insemination: a retrospective review on determinants of success Int J Reprod Contracept Obstet Gynecol. 2(3), 311-314. https://doi.org/10.5455/2320-1770.ijrcog20130910

Al-Dujaily SS, Alnakash AH, Al-Bayati S (2006) Effect of pentoxifylline on the outcome of artificial inseminations Iraqi Post Med J. 5(3), 80-86.