Glutathione S-Transferase Activity of Three Erythrocyte Genotypes of Human Participants Treated with Pyrimethamine/Sulphadoxine and Quinine

Paul Chidoka Chikezie

Authors

Paul Chidoka Chikezie

Keywords:

glutathione S-transferase activity, erythrocytes, pyrimethamine/sulphadoxine, quinine, 1-chloro-2, 4- dinitrobenzene (CDNB)

Abstract

Studies to ascertain levels of erythrocyte glutathione S-transferase (Ery-GST) activity of non-malarious and malarious male participants of HbAA, HbAS and HbSS erythrocyte genotypes treated with pyrimethamine/sulpha- doxine mixture and quinine were carried out. Incubation of erythrocytes with 1-chloro-2, 4-dinitrobenzene (CDNB) caused quantitative conjugation of reduced glutathione (GSH) to produce S-(2, 4-dinitrophenyl) glutathione, which formed the bases for the measurement of Ery-GST activity using a spectrophotometer. Blood samples were drawn from treated non-malarious and malarious participants at time intervals of 0, 3, 6 and 18 h and measured for Ery-GST activity. The control values of Ery-GST activity of non-malarious and malarious participants were within the ranges of 3.27 ± 0.13 - 12.50 ± 1.58 IU/gHb and 2.75 ± 0.16 - 12.21 ± 1.20 IU/gHb respectively. Ery-GST activity of malarious participants was significantly (p < 0.05) lower than that of the malarious participants, except that of parasitized HbSS erythrocytes. Generally, Ery-GST activities in the presence of the two antimalarials exhibited a biphasic profile. The first phase showed decreasing levels of Ery-GST activity at t < 6 h following the administration of pyrimethamine/sulphadoxine mixture and quinine to the non-malarious and malarious participants. In the second phase, Ery-GST activity increased when experimental t > 6 h. The overall pattern of Ery-GST activity within the experimental time (0 < t < 18 h) showed evidence of antimalarial induced disturbance of erythrocyte homeostasis, which could be of relevance from toxicological standpoints and for monitoring therapeutic events in malarial disease.

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Glutathione S-Transferase Activity of Three Erythrocyte Genotypes of Human Participants Treated with Pyrimethamine/Sulphadoxine and Quinine. (2014). Global Journal of Medical Research, 14(45), 5-12. https://medicalresearchjournal.org/index.php/GJMR/article/view/

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Author Biography

Paul Chidoka Chikezie

Industry: Education & Instruction, Scientific Research & Development: Senior Teaching Fellow in Biomedical Science at Coventry University, United Kingdom , Fellow of the Institute of Biomedical Science (FIBMS) and Fellow of the Higher Education Academy (FHEA); 2+ decades of teaching, research and academic leadership with substantial UK Higher Education teaching experience; Professor of Medical Biochemistry, Head of Department of Biochemistry, and Associate Dean of the Faculty of Biological Sciences at Imo State University, Owerri, Nigeria; Lecturer in Chemistry (FE); Endorsed by The Royal Society, UK, as a Global Scholar of exceptional talent in biochemistry and academic leadership; Authored 140+ publications and recipient of multiple research grants-TETFund awards; Expertise spans basic/applied biomedical science/biochemistry (metabolomics/physiological chemistry):- intermediary metabolism & bioenergetics, enzymology, molecular pathophysiology/diagnostic biochemistry, biochemical pharmacology, visceral organ toxicity assessment, cell & molecular biology, blood disorders (haemoglobinopathies, haemostasis & coagulation cascade), and related topics; Skilled in inclusive pedagogy, assessment innovation, student engagement, reflective teaching, and curriculum alignment with QAA, TEF, NSS, retention, progression, and student success. https://www.researchgate.net/profile/Paul-Chikezie

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