Plasma Total Amino Acids, Plasma Glutamate and Alanine Levels in Diabetic Subjects

Dr. Kashinath R.T.; Dr. nagendra subbaiah MD; Dr. Rudrappa. G.; Dr.

Authors

Dr. Kashinath R.T.
Dr. nagendra subbaiah MD
Dr. Rudrappa. G.
Dr.

Keywords:

amino acids, gluconeogenesis, glutamate, alanine

Abstract

Generally, in fasting state, gluconeogenesis accounts for about 30% of overall hepatic glucose output, the increase in type 2 diabetic subjects may be much more than this level. There is a close relationship between glucose metabolism and amino acid metabolism which is established through transamination reactions. The key amino acid -keto acid pairs involved in transamination reactions are glutamateα ketoglutarate, aspartate -oxaloacetate and pyruvate -alanine. A study was undertaken to assess the plasma levels of total amino acids, glutamate and alanine in type 2 diabetic subjects. A blood sample (5ml) with heparin as an anticoagulant was collected in the fasting state, from each of the selected normal as well as diabetic subjects. The separated plasma samples were employed for the estimation of total amino acid nitrogen levels, as well as for the estimation of plasma alanineand glutamate levels. The results indicate a significant rise in the plasma levels of total amino acids, alanine & glutamate in type-2 diabetic subjects as well as a significant increase in plasma alanine levels in diabetics of 30-40 yrs age as compared to diabetics of 41-60yrs of age. These findings suggest an increased availability of glucogenic amino acid precursors for glucose formation may be due to lack of proteolytic suppression of insulin.

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Plasma Total Amino Acids, Plasma Glutamate and Alanine Levels in Diabetic Subjects. (2014). Global Journal of Medical Research, 14(2), 23-27. https://medicalresearchjournal.org/index.php/GJMR/article/view/

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Author Biographies

Dr. Kashinath R.T.

Dr. Kashinath R.T. is a biochemist with an MSc in Biochemistry, a PhD in Biochemistry (Medical Faculty), and is a Fellow of the International College of Surgeons (FICS). His research focuses on diabetes, cardiovascular risks, and lipoproteins. He has served as Professor and Head of the Department of Biochemistry at Subbaiah Institute of Medical Sciences & Research Center, and as Director of R&D at SUIMS. He has published 14 papers and has an h-index of 3.

Dr. nagendra subbaiah MD

Dr. R. T. Kashinath is a Professor of Biochemistry at Subbaiah Institute of Medical Sciences. He holds an MSc and PhD in Biochemistry, and is a Fellow of the International College of Surgeons (FICS). His research interests include membrane phospholipid/cholesterol ratio, thiol-disulfide balance, and endocrinology, diabetes and metabolism.

Dr. Rudrappa. G.

Dr. R. T. Kashinath is a researcher in medical biochemistry with a focus on Type 2 Diabetes Mellitus, membrane lipid alterations, thiol-disulfide balance, and the effects of organosulfur compounds. He has held positions at Basaveshwara Medical College & Hospital, BLDE University, Vijayanagara Institute of Medical Sciences, and Subbaiah Institute of Medical Sciences & Research Center, all in India. He holds an MSc in Biochemistry, a PhD in Biochemistry (Medical Faculty), and is a Fellow of the Indian College of Surgeons (FICS).

Dr.

Dr. R. T. Kashinath is a Professor and Head of the Department of Biochemistry at Subbaiah Institute of Medical Sciences, Shimoga, Karnataka, India. He holds an MSc in Biochemistry, a PhD in Biochemistry (Medical Faculty), and is a Fellow of the Indian College of Surgeons (FICS).

References

Agostino consoli, Nurjahan Nurjahan, J. James, Reilly, M. Dennis, John Beir, Gerich (1990) Mechanism of increased gluconeogenesis in noninsulin dependent diabetes mellitus. J .Clin. 86.

Best J, Judezze R, Pfeifer. M, Beard J, Halter J, Porte. D (1982) The Effect of Chronic Sulfonylurea Therapy on Hepatic Glucose Production in Non-insulin-dependent Diabetes. 31(4), 333-338. https://doi.org/10.2337/diab.31.4.333

Campbell P, Mandarino L, Gerich J (1988) Quantification of the relative impairment in actions of insulin on hepatic glucose production and peripheral glucose uptake in non-insulin-dependent diabetes mellitus. 37, 15-22.

Consoli A, Nurjahan N, Capani F, Gerich J (1989) Predominant role of gluconeogenesis in increased hepatic glucose production in NIDDM. 38(5), 550-557. https://doi.org/10.2337/diabetes.38.5.550

DeFronzo R, Gunnarsson R, Bjorkman O, Olsson M, Wahren J (1985) Effects of insulin on peripheral and splanchnic glucose metabolism in noninsulin-dependent (type II) diabetes mellitus. 76(1), 149-155. https://doi.org/10.1172/jci111938

DeFronzo R, Simansson D, Ferrannini E (1982) Hepatic and peripheral insulin resistance: A common feature of Type 2 (non-insulin-dependent) and Type 1 (insulin-dependent) diabetes mellitus. 23(4), 313-319. https://doi.org/10.1007/bf00253736

R. DeFranzo, A. Golay, J. Felbar (1985) Glucose and lipid metabolism in obesity and diabetes mellitus in "Substrate and energy metabolism. 70-81.

Firth R.G, Bell P.M, Hanson Marsh H.M, Rizza R.A (1986) Post-Prandial hyperglycemia in patients with non insulin dependent diabetes mellitus. Role of hepatic and extra hepatic tissues. J. Clin. 77(5), 1525-1532. https://doi.org/10.1172/jci112467

Folin (1965) cited in "Hawk's physiological chemistry. 1048-1050.

P. Fao, G. Galasino, Costa (1955) Am. j. 182, 493-496.

R. Fulks, J. Li, A. Goldberg (1975) Effect of insulin, glucose and amino acids on protein turnover in rat diaphragm. 250(1), 290-298. https://doi.org/10.1016/s0021-9258(19)42013-9

Mirakou Gerich J, Mandarino Kelley D, Reilly Nurjahan N, Veneman Jenssen T, Consoli A (1990) Contribution of impaired muscle glucose clearance to reduced postabsorptive systemic glucose clearance in NIDDM. 39(2), 211-216. https://doi.org/10.2337/diabetes.39.2.211

Goldstein J.L, Brown M.S, etal Anderson R.G (1995) Receptor-Mediated Endocytosis: Concepts Emerging from the LDL Receptor System. 1(1), 1-39. https://doi.org/10.1146/annurev.cb.01.110185.000245

R. Garcia, V. Gazola, H. Barrena, E. Hartmann, J. Berti, M. Toyama, A. Boschero, E. Carneiro, F. Manso, R. Bazotte (2007) Blood amino acids concentration during insulin induced hypoglycemia in rats: the role of alanine and glutamine in glucose recovery. 33(1), 151-155. https://doi.org/10.1007/s00726-006-0397-8

L. Jefferson, D. Rannels, B. Munger, Morgan (1974) Insulin in the regulation of protein turnover in the heart and skeletal muscle. 33, 1098-1104.

R. Kries berg (1972) Glucose - Lactate interrelations in man. N. Eng.J. 287, 132-137.

Loundholm K, Edstrom S, Ekman L, Karlerg I, Walker P, Schersten T (1981) Protein Degradation in Human Skeletal Muscle Tissue: The Effect of Insulin, Leucine, Amino Acids and Ions. 60(3), 319-326. https://doi.org/10.1042/cs0600319

Richard Block, L. Emmett Durram & Guntar Zweig cited in "A Manual of paper Chromatography and paper electrophoresis. Page, 143-151.

Sudharland E.W, Cori C.F (1951) J. Biol. 188, 531-534.

P. Vishweshwaran, K. Subramanyan (1970) Proc. Soc.Biol. Chemistry. 29, 31.