Modifications and Optimization of an Autologous Intracerebral Hemorrhage Rat Model

Abstract

Objective To date no novel drug therapies have proven effective in improving outcomes after intracerebral hemorrhage ICH This has been slowed in part by a lack of reliable preclinical models that recapitulate the pathophysiological consequences of ICH Limited descriptions of the methodology of preclinical models may play a role in this failed translation The current study aimed to improve the autologous blood injection model of ICH and provide a comprehensive methodology to facilitate a reliable preclinical model that bridges the translational research gap Methods A modified single-injection protocol for an autologous-blood ICH model was developed and tested This rodent ICH model produces injury in the basal ganglia of adult rats via a stereotaxically-assisted right-sided single-injection of fresh autologous whole blood