Performing the Garcia score for accuracy and reliability

Abstract

Reliable sensitive and accurate tests are needed to assess animal models of brain injury The Garcia score is a neurobehavioral measure that has been used in many murine studies However despite its widespread use there are no detailed video descriptions of the steps to properly perform and grade the Garcia test on rats Consequently there has been significant variation in its performance and reliability calling for greater standardization The Garcia score is comprised of six measures spontaneous activity symmetry in the four limbs forepaw outstretching climbing body sensation and response to vibrissae touch Each component is scored with a minimum of zero or one and a maximum of three with the highest total score of 18 This report systematically and clearly describes how each component of the Garcia score is performed and graded with an accompanying video illustration