Characterization and Antibiotics SensitivityPatternof Enterobacteriaceae in Obafemi Awolowo University Sewage Oxidation Pond

Sokan-Adeaga Micheal Ayodeji; Sokan-Adeaga Adewale Allen; Sokan-Adeaga Eniola Deborah

Authors

Sokan-Adeaga Micheal Ayodeji
Sokan-Adeaga Adewale Allen
Sokan-Adeaga Eniola Deborah

Keywords:

enterobacteriaceae, sewage oxidation pond, total bacteria count, antibiotic sensitivity, biochemical characterization

Abstract

Some members of the family Enterobacteriaceae were isolated from Obafemi Awolowo University sewage oxidation pond (target site) and characterize by various biochemical test (citrate test, Gram stain, catalase test, idole production, fermentation of sugar, starch hydrolysis, Nitrate reduction, oxidative-fermentative test, gelatin hydrolysis and Methyl Red Voges-Proskauer test); the antibiotic sensitivity pattern of the isolate were also carried out. All was done under an aseptic condition. The total bacterial count (TBC) obtained from the target site was higher than those obtained from the relative site (1: before the sewage enter) and (2: after leaving) the oxidation pond. The biochemical tests identified the following: Citrobacter diversus, Salmonella arizonae, Typical Salmonella, Escherichia coli and Providencia alcalifaciens, all belonging to the family Enterobacteriaceae. Investigation of the antibiotic sensitivity test revealed that Cefuroxime and Ampicillin were not effective against all the bacteria isolates.

Downloads

How to Cite

Characterization and Antibiotics SensitivityPatternof Enterobacteriaceae in Obafemi Awolowo University Sewage Oxidation Pond. (2018). Global Journal of Medical Research, 18(C1), 7-17. https://medicalresearchjournal.org/index.php/GJMR/article/view/1549

Download Citation

References

S Al-Bahry, A Elshafie, R Victor, I Mahmoud, J Al-Hinai (2011) Opportunistic pathogens relative to physicochemical factors in water storage tanks. 9, 382-393.

M Al-Hasan, B Lahr, J Eckel-Passow, L Baddour (2011) Temporal trends in Enterobacter species bloodstream infection: a population-based study from 1998-2007. 17(4), 539-545.

R Atlas, R Bartha (1997) Microbial ecology fundamentals and applications.

Alexander Auch, Mathias Von Jan, Hans-Peter Klenk, Markus Göker (2010) Digital DNA-DNA hybridization for microbial species delineation by means of genome-to-genome sequence comparison. 2(1), 117-134.

G Balows (1991) Manual of Clinical Microbiology. 367-370.

Diana Borenshtein, David Schauer (2006) The Genus Citrobacter. 6, 90-98.

T Brock, M Madigan (1998) Biology of micro organisms. 550-659.

G Brooks, J Butel, S Morse (2003) Jawetz, Mel nick and Adeberg. 23, 248-264.

S Brisse, F Grimont, P Grimont (2006) The prokaryotes: proteobacteria: gamma subclass. 6, 159-196.

Cheng-Hsun Chiu, Lin-Hui Su, Chishih Chu (2004) Salmonella entericaSerotype Choleraesuis: Epidemiology, Pathogenesis, Clinical Disease, and Treatment. 17(2), 311-322.

M Croxen, B Finlay (2010) Molecular mechanisms of Escherichia coli pathogenicity. 8, 26-38.

M Denton (2007) Enterobacteriaceae. 29(Suppl 3), 9-S22.

J Farmer, M Kelly (1991) Enterobacteriaceae. 360-383.

T Felfoldi, Z Heeger, M Vargha, K Marialigeti (2010) Detection of potentially pathogenic bacteria in the drinking water distribution system of a hospital in Hungary. 16, 89-92.

Joseph Gillespie, Alice Wattam, Stephen Cammer, Joseph Gabbard, Maulik Shukla, Oral Dalay, Timothy Driscoll, Deborah Hix, Shrinivasrao Mane, Chunhong Mao, Eric Nordberg, Mark Scott, Julie Schulman, Eric Snyder, Daniel Sullivan, Chunxia Wang, Andrew Warren, Kelly Williams, Tian Xue, Hyun Seung Yoo, Chengdong Zhang, Yan Zhang, Rebecca Will, Ronald Kenyon, Bruno Sobral (2011) PATRIC: the Comprehensive Bacterial Bioinformatics Resource with a Focus on Human Pathogenic Species. 79(11), 4286-4298.

A Ghrabi, M Ferchichi, C Drakidès (1993) Treatment of Wastewater by Stabilization Ponds - Application to Tunisian Conditions. 28(10), 193-199.

Francine Grimont, Patrick Grimont (2006) The Genus Enterobacter. 6, 197-214.

J Hedegaard, S Steffensen, N Norskov-Lauritsen, K Mortensen, H Sperling-Petersen (1999) Identification of Enterobacteriaceae by partial sequencing of the gene encoding translation initiation factor 2. 49(Pt 4), 1531-1538.

Tara Houndt, Howard Ochman (2000) Long-Term Shifts in Patterns of Antibiotic Resistance in Enteric Bacteria. 66(12), 5406-5409.

B Hosetti, S Frost (1995) A review of the sustainable value of effluents and sludges from wastewater stabilization ponds. 28, 421-431.

J Janda (2006) New members of the family Enterobacteriaceae. 6, 5-40.

Konstantinos Konstantinidis, James Tiedje (2005) Genomic insights that advance the species definition for prokaryotes. 102(7), 2567-2572.

M Madigan, J Martinko, J Parker (1997) Brock biology of microorganism. 466-467.

M Madigan, J Martinko, P Dunlap, D Clark (2008) Brock Biology of Microorganisms. 12.

C Mahajan, S Narkhade, V Khatik, R Jadhav, S Attarde (2010) Wastewater treatment at winery industry. 4(2), 258-265.

G Morrison, O Fatoki, Persson, A Ekberg (2001) Assessment of the impact of point source pollution from the Kieskanmahoek sewage treatment plant on the Kieskanmahoek river -ph, electrical conductivity, oxygen demanding substance and nutrients. 27, 475-480.

G Nair (1997) Role of organisms in sewage treatment I: Types of organisms. 6(1), 19-26.

E Nester, D Anderson, C Roberts, Jr, N Pearshall, M Nester (2001) Unknown Title. 787-798.

A Olajire, T Impekperia (2000) A study of water quality of the Osun River; metal monitoring and geochemistry. 14, 1-18.

Sonia Paradis, Maurice Boissinot, Nancy Paquette, Simon Bélanger, Eric Martel, Dominique Boudreau, François Picard, Marc Ouellette, Paul Roy, Michel Bergeron (2005) Phylogeny of the Enterobacteriaceae based on genes encoding elongation factor Tu and F-ATPase β-subunit. 55(5), 2013-2025.

D Paterson (2006) Resistance in gram-negative bacteria: Enterobacteriaceae. 34, 64-S73.

H Pham, K Ohkusu, N Mishima, M Noda, M Monir Shah, X Sun, M Hayashi, T Ezaki (2007) Phylogeny and species identification of the family Enterobacteriaceae based on dnaJ sequences. 58, 153-161.

B Picot, A Bablaoui, B Moersidik, Baleux, J Bontoux (1992) Comparison of the purifying efficiency of high rate algal pond with stabilization pond. 25, 12.

J Pitout (2008) Multiresistant Enterobacteriaceae: new threat of an old problem. 6, 657-669.

L Prescott, J Harley, D Klein (2005) Unknown Title. 926-932.

F Reinthaler, J Posch, G Feierl, G Wüst, D Haas, G Ruckenbauer, F Mascher, E Marth (2003) Antibiotic resistance of E. coli in sewage and sludge. 37(8), 1685-1690.

M Von Sperling, C De Lemos Chernicharo (2005) Biological Wastewater Treatment in Warm Climate Regions Volume II. 4(0), 9781780402703-9781780402703.

T Tennstedt, R Szczepanowski, S Braun, A Pühler, A Schlüter (2003) Occurrence of integronassociated resistance gene cassettes located on antibiotic resistance plasmids isolated from a wastewater treatment plant. 45, 239-252.

N Tharavathy, B Hosetti (2003) Biodiversity of algae and protozoa in a natural waste stabilization pond: A field study. 24(2), 193-199.