Background: Uterine prolapse (UP) is a common reproductive health problem in lowmid- income countries such as Nepal. The current study aimed to explore the factors associated with knowledge regarding UP among women attending the gynecology outpatient department of tertiary care teaching hospital. Methods: A cross-sectional study was conducted among patients visiting the gynecology outpatient department of tertiary care teaching hospital in Nepal from July to September, 2016 using a purposive sampling technique. A suitably designed and validated questionnaire of knowledge consisting of 20 questions was used to determine the knowledge scores. Descriptive statistics and bivariate analysis were used; a p-value <0.05 was taken as significant in multivariate analysis.