Background: Variation of microalgae cultivation conditions affects biosynthetic processes, which leads to a change in the content and qualitative composition of lipids - the raw material for the biodiesel production. The cultivation of microalgae biomass requires mineral nutrition, to provide it natural sources of salts and chemically synthesized compounds are used. The use of agricultural waste will reduce the anthropogenic burden on the environment, reduce the biofuels cost and will help to solve the problems of alternative energy from renewable raw materials production. In this case, in addition to microalgae, it is possible to obtain biologically active substances that can be used as a feedstock for animals and poultry. Objective: The purpose was to study the Chlorella vulgaris microalgae cultivation while using the extract from poultry droppings as a nutrient medium and its effect on lipid fraction content in the cells. The subject of the study is Chlorella vulgaris development and lipid accumulation in the cells.