Abstract

Background: Although there are many motility stimulants have been found to play an important role in improving the active sperm motility and morphologically normal sperm (MNS) percentages when added in vitro with the culture medium for semen ,the results of pregnancy rate following intra-uterine insemination (IUI)still not exceed 20% . Objective: This study is a trial to improve the results of pregnancy rate following IUI by adding the follicular fluid (FF),and pentoxifylline (PX) to the culture medium used for preparation of asthenozoospermic semen samples. Subjects, Material and methods: Ninety five infertile couples were involved in the current study (asthenozoospermic male partners with intact spouses) divided into three groups according to the sperm stimulants which were used for in vitro sperm activation followed by intrauterine insemination technique. Group one was FF-treated semen samples and consists of 40 couples, group two was FF+PX-treated semen samples and consists of 25 couples and lastly, group three was Ham's F-12 culture medium as a control group and consists of 30 couples. All the couples were followed up for determining outcome of pregnancy after IUI was done. Results: Out of the all 95 couples, 22 pregnancies (23.15%) were resulted following IUI. The best results were obtained by group one (FF treated group) in which out of 40 cases enrolled in this group, 12 pregnancies (30%) were resulted following IUI. Regarding group two (FF+PX treated group), out of 25 cases enrolled in this group, 5 women (20%) were pregnant following IUI. Regarding group three (Ham's F-12 treated group), out of 30 cases enrolled in this group, 5 women (16.67%) were pregnant following IUI. Conclusion: Adding FF to culture media for in vitro sperm activation was associated with a higher pregnancy rate following IUI than other treated culture media.