In Vitro Sperm Preparation by Progesterone, Pentoxifylline and Glycyrrhiza Glabra for Asthenozoospermic Men
Keywords:
glycyrrhiza glabra, in vitro sperm preparation, pentoxifylline, progeste- rone
Abstract
Results of artificial insemination (AI) still does not exceed 20-25% because of different factors one of them is male factor infertility. Therefore, the global companies concern on the culture media that used for activation of semen samples of infertile men. However, till now, there are many semen samples did not response to these media leading to failure in AI. The objective of this study is to improve certain sperm function parameters in vitro of asthenoteratospermic patients by using novel combination motility stimulants substances that improved medium to be used for assisted reproductive technologies (such as artificial insemination) in future. One hundred asthenozoospermic patients are involved in this study. They are divided randomly into equal four groups. Each semen sample had been divided into two parts. One part was considered as a control by using Ham's F 10 medium and the other part is considered as treated group by adding the following substances in combination; Pentoxifylline (PF) 1 mg/ml, Progesterone (P) 0.409 mg/ml and Glycyrrhiza glabra (Gg) 1 mg/ml in the following groups. Group 1:PF and Gg, Group 2: PF and P,Group 3: Gg and P, and Group 4:PF,Gg and P. Sperm function parameters are examined before and following in vitro preparation using wash and spin technique. The results revealed highly significant increment (P
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A. Agarwal, S. Prabakkaran (2005) Oxidative stress and antioxidative in male infertility: a difficult balance. Iranian J. Reprod. 3, 1-8.
S. Al-Dujaily, A. Al-Saadi (2009) Effect of Glycyrrhiza glabra extract on IVF outcome in Mice : Experimental model for mammals. (2), 80-89.
S. Al-Dujaily, R. AL-Shammary (2008) Effect of modified Tris solution with Glycyrrhiza glabra extract on cryostorage of infertile semen several days after ejaculation. 106-112.
S. Al-Dujaily, M. Al-Brazanchi, M. Fakhridin (1999) Use of mice oviductal flushing medium in vitro to activate the human and mice sperm function. 4, 508-514.
S. Al-Dujaily, A. Al-Janabi, M. Nori (2006) Effect of Glycyrrhiza extract on in vitro sperm activation of asthenospermic patients. 11(3), 477-483.
S. Al-Dujaily, A. Al-Nakash, S. Al-Biaty (2007) Effect of Pentoxifylline on the outcome of artificial inseminations. Iraqi Postgraduate Mid. 5(4), 377-383.
A. Allaw (1999) Treatment and in Vitro Sperm Activation for Immunologically Infertile Patients.
J. Bonde, E. Ernst, T. Jensen (1998) Relation between semen quality and fertility: a population-based study of 430 first-pregnancy planners. 10, 1172-1176.
H. Chakravaarty (1976) Plant Wealth of Iraq. A Dictionary of Economic Plants, Ministry of Agriculture and Agrarian Reform. 25-261.
J. Check, P. Zavos, D. Katsoff, D. Kiefer (1993) Effect of Percoll discontinuous density gradients Vs sperm prep II Vs saphedeax G-50 gel filtration on sperm parameters. JEXP. 169, 225-231.
J. Check, A. Bollendorf, M. Pres, T. Blue (1992) Standard Sperm Morphology as a Predictor of Male Fertility Potential. 28(1), 39-41. https://doi.org/10.3109/01485019208987678
H. Chemes, S. Olmedo, C. Carrere, R. Oses, C. Carizza, M. Leisner, J. Blaquier (1998) Ultrastructural pathology of the sperm flagellum: association between flagellar pathology and fertility prognosis in severely asthenozoospermic men. 13(9), 2521-2526. https://doi.org/10.1093/humrep/13.9.2521
K. Elder, S. Avery, C. Mills (1999) Laboratory Procedures.
N. Kaewnoonual, C. Chiamchanya, P. Visutakul, S. Manochantr, J. Chaiya, P. Tor-Udom (2008) Comparative study of semen quality between pre-washed and post-washed with 3 sperm preparation media Thammasat Med. 8(3), 293.
V. Karpuz, A. Gokturk, M. Koyuturk (2007) The Effect of Sperm Morphology and Motility on THE Outcome of Intracytoplasmic Sperm Injection .Marmara Med. 20(2), 92-97.
Patricia Katz, Robert Nachtigall, Jonathan Showstack (2002) The economic impact of the assisted reproductive technologies. 4, S29-S32. https://doi.org/10.1038/ncb-nm-fertilitys29
T. Mehrannia (2009) The Effect of Pentoxifylline in semen preparation for intrauterine insemination. Pak, J. Med. 25(3), 359-363.
S. Mesiano (2001) Roles of Estrogen and Progesterone in Human Parturition. 27, 86-104.
A. Nassar, M. Mahony, P. Blackmore, M. Morshedi, K. Ozgur, S. Oehninger (1998) Increase of intracellular calcium is not a cause of pentoxifyllineinduced hyperactivated motility or acrosome reaction in human sperm. Fertil Steril. 69(4), 748-754.
A. Pacey (2006) Is quality assurance in semen analysis still really necessary? A view from the andrology laboratory. 21, 1105-9.
Sjoerd Repping, Janne-Meije Van Weert, Ben Mol, Jan de Vries, Fulco van der Veen (2002) Use of the total motile sperm count to predict total fertilization failure in in vitro fertilization. 78(1), 22-28. https://doi.org/10.1016/s0015-0282(02)03178-3
E. Sheena, M. James, T. William (1993) Effect of pentoxifylline on human sperm motility in normospermic individuals using computer-assisted analysis.
D. Sorlie (1995) Medical Biostatistics and Epidemiology: examination and board review. 1st edition. Appleton and Lang, Norwalk. 47-88.
T. Stalf, C. Mehnert, A. Hajimohammad, K. Manolopoulos, Y. Shen, H-C Schuppe, T. Diemer, W-B Schill, W. Weidner, H-R Tinneberg (2005) Influence of motility and vitality in intracytoplasmic sperm injection with ejaculated and testicular sperm. 37(4), 125-130. https://doi.org/10.1111/j.1439-0272.2005.00665.x
L. Taylor (2004) Database File for Licorice. http://www.rain-tree.com./licorice.html
J. Tesarik, C. Mendoza, A. Carreras (1992) Effects of phosphodiesterase inhibitors caffeine and pentoxifylline on spontaneous and stimulus-induced acrosome reactions in human sperm. 58, 1185-1189.
J. Tesarik, A. Thebauld, J. Testart (1992) Effect of pentoxifylline on sperm movement characteristics in normozoospermic and asthenozoospermic specimens. 7, 1257-1262.
A. Ward, S. Clissold (1987) Pentoxifylline: A review of its pharmacodynamic and pharmacokinetic properties, and its therapeutic efficacy. 34, 50-97.
World Health Organization (WHO) (1999) World Health Organization Laboratory Manual for the Examination of Human Semen and Sperm‐Cervical Mucus Interaction, 4th ed.
S. Yalti, B. Gurbuz, H. Sezer, S. Celik (2004) EFFECTS OF SEMEN CHARACTERISTICS ON IUI COMBINED WITH MILD OVARIAN STIMULATION. 50(4), 239-246. https://doi.org/10.1080/01485010490448435
R. Yunes, G. Doncel, A. Acosta (2003) Incidence of sperm-tail tyrosine phosphorylation and hyperactivated motility in normozoospermic and asthenozoospermic human sperm samples. 27(1), 29-36. https://doi.org/10.32604/biocell.2003.27.029
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2013-11-04
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