Efficacy of Antirabies IgG and IgY on Protection of Mice against Experimental Viral Infection as a Model for Emergency Intervention

Abstract

Aim: Preparation of anti-rabies IgY in chicken egg yolk in addition to preparation of antirabies IgG in rabbits and evaluate their efficacy in protection against rabies infection in emergency cases simulated in mice. Materials and Methods: Fifteen, 35week-old, Rhode Island Red (RIR) hens were used for preparation of anti-rabies IgY from egg yolk. It was found that the obtained IgY had a concentration of 2.32 g/dl. In addition, ten Bosket rabbits of about 3kg bodyweight were used for preparation of anti-rabies IgG which was found to have a concentration of 3.42g/dl. Both of chicken egg yolk IgY and rabbit IgG were found to be safe when they are inoculated intraperitoneally in mice. Results: Serum neutralization test revealed that IgY and IgG had rabies antibody titers of 64 and 128 respectively. The tow preparations were tested for determination of their potency in experimentally infected mice with rabies virus on daily intervals post infection.