The Average of HBA among Selected Group of Sudanese Pateints with Fertility Disorders
Keywords:
fertility rate, urban, rural, hyaluronan binding assay
Abstract
Background: Sperm hyaluronan binding may be an indicator of which sperm are most likely to produce a viable pregnancy. For example, mature, hyaluronan-binding sperm are essentially free of cytoplasmic inclusions. This is of great value since cytoplasmic inclusions are extremely difficult to see when selecting sperm to inject into eggs in an in vitro fertilization cycle. Rationale: There is no known data about the average of HBA among Sudanese patients with infertility problems. Objectives: To know the average of HBA among selected group of Sudanese patients with fertility disorders. Method: Men who are preparing to do in vitro fertilization with ICSI will be asked to collect a semen specimen in the same manner that they would for a conventional semen analysis. The semen is mixed with some media and placed on a special slide that has been coated with hyaluronan. Mature sperm will bind the hyaluronan (bound). These sperm will appear to have their heads stuck but with tails that show vigorous tail motion. Immature sperm will move freely (unbound Result: Average of HBA was 74.82% Discussion: The average of HBA among the participants was higher than normal and that means the reproduction disorder may probably occur in their females partners. Conclusion: We conclude that the average of HBA among the participants was normal. Recommendations: Further studies must be done with large sample size and the females partners should be diagnosed for infertility disorders. Acknowledgement: To all participants in the study for their collaboration and cooperation with the research team.
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2018-03-15
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