Comparative Studies on the Antioxidant enzymes in Bone Marrow Precursor cells and mature Rat Polymorphonuclear Leukocytes

Ms Swechha Mishra

Authors

Ms Swechha Mishra

Keywords:

bone marrow precursor cells, polymorphonuclear leukocytes, antioxidants, nitric oxide

Abstract

Continuous blood cell production throughout the lifetime of an individual is ensured by the hematopoietic stem cells (HSCs), which are bone marrow cells that possess extensive self renewal capacity and ability to differentiate to all the blood cell lineages. The redox status of the bone marrow stroma is an important factor determining whether the HSCs would differentiate or remain quiescent. Lower levels of ROS (reactive oxygen species) are required to maintain HSC quiescence, whereas higher ROS levels drive the HSCs out of quiescence and reduces their self renewal capacity. The ROS levels in the bone marrow should therefore be regulated to maintain the self renewal capacity of HSCs. Endogenous antioxidants play an important role in regulation of the ROS levels. The present study was therefore undertaken to examine the changes in the cellular detoxifying defences against superoxide and H2O2 i.e. superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx), in the precursor cells from the rat bone marrow and in the rat blood polymorphonuclear leukocytes. An increase in the Catalase and GPx activities in the precursor cells over the differentiated cells was seen. However, the SOD levels in the differentiated cells were greater than in the precursor cells. The levels of Nitric Oxide (NO)-an antioxidant and a regulator of superoxide generation-in the precursor cells were also found to be greater than those in the differentiated cells. The results of the present study thus indicate an up regulation of the endogenous antioxidants in hematopoietic precursor cells, which might be related to the maintenance of their self-renewal capacity.

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Comparative Studies on the Antioxidant enzymes in Bone Marrow Precursor cells and mature Rat Polymorphonuclear Leukocytes. (2018). Global Journal of Medical Research, 18(K3), 17-21. https://medicalresearchjournal.org/index.php/GJMR/article/view/1524

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References

J Karlsson (1997) Introduction to Nutraology and Radical Formation. 1-143.

Florian Muller (2000) The nature and mechanism of superoxide production by the electron transport chain: Its relevance to aging. 23(4), 227-253.

Carroll Cross, Barry Halliwell, Edward Borish, William Pryor, Bruce Ames, Robert Saul, Joe Mccord, Denham Harman (1987) Oxygen Radicals and Human Disease. 107(4), 526-545.

B Aims, M Shinegava, T Hagen (1993) Oxidants, antioxidants and the degenerative diseases. 90, 7915-7922.

Shugo Ueda, Hiroshi Masutani, Hajime Nakamura, Toru Tanaka, Masaya Ueno, Junji Yodoi (2002) Redox Control of Cell Death. 4(3), 405-414.

E Boon, A Downs, D Marcey (2007) Catalase Structural Tutorial Text. 5, 9-15.

Jack Cowland, Niels Borregaard (1999) Isolation of neutrophil precursors from bone marrow for biochemical and transcriptional analysis. 232(1-2), 191-200.

S Greenberg, J Xie, X Zhao, O Jie, T Giles (1996) An in vivo cytokine and endotoxinindependent pathway for induction of nitric oxide synthase II mRNA, enzyme, and nitrate/nitrite in alveolar macrophages. 227, 160-161.

Stephen Pember, Raymond Shapira, Joseph Kinkade (1983) Multiple forms of myeloperoxidase from human neutrophilic granulocytes: Evidence for differences in compartmentalization, enzymatic activity, and subunit structure. 221(2), 391-403.

P Kakkar, B Das, P Viswanathan (1984) A modified spectrophotometric assay of superoxide dismutase. 2, 130-132.

Hugo Aebi (1984) [13] Catalase in vitro. 105, 121-126.

Sunita Sheokand, Anita Kumari, Veena Sawhney (2008) Effect of nitric oxide and putrescine on antioxidative responses under NaCl stress in chickpea plants. 14(4), 355-362.

Hans-Georg Kopp, Scott Avecilla, Andrea Hooper, Shahin Rafii (2005) The Bone Marrow Vascular Niche: Home of HSC Differentiation and Mobilization. 20(5), 349-356.

Toshio Suda, Fumio Arai, Shigeto Shimmura (2005) Regulation of Stem Cells in the Niche. 24(8), S12-S17.

Zhixing Li, Linheng Li (2006) Understanding hematopoietic stem-cell microenvironments. 31(10), 589-595.

G Saretzki, L Armstrong, A Leake, M Lako, Von Zglinicki, T (2004) Stress defense in murine embryonic stem cells is superior to that of various differentiated murine cells. 22, 962-971.

Safak Yalcin, Xin Zhang, Julia Luciano, Sathish Mungamuri, Dragan Marinkovic, Cécile Vercherat, Abby Sarkar, Marcos Grisotto, Reshma Taneja, Saghi Ghaffari (2008) Foxo3 Is Essential for the Regulation of Ataxia Telangiectasia Mutated and Oxidative Stress-mediated Homeostasis of Hematopoietic Stem Cells. 283(37), 25692-25705.

J Breton-Gorius, J Guichard (1975) Fine structural and cytochemical identification of microperoxisomes in developing human erythrocytic cells. 79, 523.

A Marcus, D Zucker-Franklin, L Safier, H Ullman (1966) Studies on human platelet granules and membranes.. 45(1), 14-28.

M Denton, N Spencer, Hrv Arnstein (1975) Biochemical and enzymic changes during erythrocyte differentiation. The significance of the final cell division. 146(1), 205-211.

J Breton-Gorius, W Vainchenker (1986) Expression of platelet proteins during the in vitro and in vivo differentiation of megakaryocytes and morphological aspects of their maturation. 23, 43.

G Magrinat, S Mason, P Shami, J Weinberg (1992) Nitric oxide modulation of human leukemia cell differentiation and gene expression. 80(8), 1880-1884.

K Lin, G Austin (2002) Functional activity of three distinct myeloperoxidase (MPO) promoters in human myeloid cells. 16(6), 1143-1153.

M Yim, P Chock, E Stadtman (1993) Enzyme function of copper,zinc superoxide dismutase as a free radical generator. 268, 4099-4105.

M Peled-Kamar, J Lotem, I Wirguin, L Weiner, A Hermalin, Y Groner (1997) Oxidative stress mediates impairment of muscle function in transgenic mice with elevated level of wild-type Cu/Zn superoxide dismutase. 94(8), 3883-3887.

Katalin Nagy, Gabriella Pásti, László Bene, Imre Nagy (1993) Induction of Granulocytic Maturation in HL-60 Human Leukemia Cells by Free Radicals: A Hypothesis of Cell Differentiation Involving Hydroxyl Radicals. 19(1), 1-15.

Elevter Russanov, Margarita Kirkova, Milka Setchenska, Henry Arnstein (1981) Enzymes of oxygen metabolism during erythrocyte differentiation. 1(12), 927-931.