Performance of various TaqMan and SYBR Green rRT-PCR methods in detecting the Egyptian foot-and-mouth disease viruses

Authors

  • Sonia A Rizk

  • Hany I Abu-Elnaga

Keywords:

foot-and-mouth disease virus, rRT-PCR, taqman

Abstract

Proficient application of the advanced technology for foot-and-mouth disease virus (FMDV) detection, still a target challenge in Egypt, not only for rapid, identification of the transboundary continuously evolving virus, but also support accurate control strategy involving manufacturing of a high-quality protective vaccine. The article discussed both two real-time RT-PCR (rRT-PCR) methods depending on either fluorophore or fluorescent dye for the precise detection of FMDV few copies. TaqMan based probe/primer overcame SYBR Green-based primer rRT-PCR by 10-fold for detecting less FMDV nucleic acid. In contrary, lower threshold cycles values with fewer nucleic acid copies biased to the fluorescent RRT-PCR with 3-4 cycles earlier. TaqMan format had better sensitivity and specificity, while SYBR Green method displayed less detection limit and precision.

How to Cite

Sonia A Rizk, & Hany I Abu-Elnaga. (2018). Performance of various TaqMan and SYBR Green rRT-PCR methods in detecting the Egyptian foot-and-mouth disease viruses. Global Journal of Medical Research, 18(G2), 1–5. Retrieved from https://medicalresearchjournal.org/index.php/GJMR/article/view/1602

Performance of various TaqMan and SYBR Green rRT-PCR methods in detecting the Egyptian foot-and-mouth disease viruses

Published

2018-05-15