Performance of various TaqMan and SYBR Green rRT-PCR methods in detecting the Egyptian foot-and-mouth disease viruses

Sonia A Rizk; Hany I Abu-Elnaga

Authors

Sonia A Rizk
Hany I Abu-Elnaga

Keywords:

foot-and-mouth disease virus, rRT-PCR, taqman

Abstract

Proficient application of the advanced technology for foot-and-mouth disease virus (FMDV) detection, still a target challenge in Egypt, not only for rapid, identification of the transboundary continuously evolving virus, but also support accurate control strategy involving manufacturing of a highquality protective vaccine. The article discussed both two real-time RT-PCR (rRT-PCR) methods depending on either fluorophore or fluorescent dye for the precise detection of FMDV few copies. TaqMan based probe/primer overcame SYBR Green-based primer rRT-PCR by 10-fold for detecting less FMDV nucleic acid. In contrary, lower threshold cycles values with fewer nucleic acid copies biased to the fluorescent RRT-PCR with 3-4 cycles earlier. TaqMan format had better sensitivity and specificity, while SYBR Green method displayed less detection limit and precision.

Downloads

How to Cite

Performance of various TaqMan and SYBR Green rRT-PCR methods in detecting the Egyptian foot-and-mouth disease viruses. (2018). Global Journal of Medical Research, 18(G2), 1-5. https://medicalresearchjournal.org/index.php/GJMR/article/view/1602

Download Citation

References

Abu-Elnaga Hi (2011) Single tube RT-PCR for simultaneous differentiation of foot-and-mouth disease virus O and An isolated in 2009 in Egypt. 8, 30-35.

A Azab, H Abu-Elnaga, A Zakria (2012) Detection of foot-and-mouth disease virus (FMDV) by RRT-PCR in Egypt. 15(1), 61-70.

Johnny Callahan, Fred Brown, Fernando Osorio, Jung Sur, Ed Kramer, Gary Long, Juan Lubroth, Stefanie Ellis, Katina Shoulars, Kristin Gaffney, Daniel Rock, William Nelson (2002) Use of a portable real-time reverse transcriptasepolymerase chain reaction assay for rapid detection of foot-and-mouth disease virus. 220(11), 1636-1642.

Laila El-Shehawy, Hany Abu-Elnaga, Sonia Rizk, Ahmed Abd El-Kreem, A Mohamed, Hossam Fawzy (2014) Molecular differentiation and phylogenetic analysis of the Egyptian foot-and-mouth disease virus SAT2. 159(3), 437-443.

M Greenwood (1927) Some Epidemiological Observations on Foot-and-Mouth Disease, with Special Reference to the Recent Experience of Holland. 26(4), 465-489.

Laila El-Shehawy, H Abu-Elnaga, A Talat, E El-Garf, A Zakria, A Azab (2011) A nucleotide sequencing of foot-and-mouth disease virus Egyptian strains. 7(7), 430-435.

Oie (2017) International Harmonization of Standards for Diagnostic Tests and Vaccines: Role of the Office International des Epizooties (OIE). 669-679.

O Othman, M Khodary, A El-Deeb, H Hussein (2018) Five BoLA-DRB3 genotypes detected in Egyptian buffalo infected with Foot and Mouth disease virus serotype O.

Scott Reid, Nigel Ferris, Geoffrey Hutchings, Zhidong Zhang, Graham Belsham, Soren Alexandersen (2002) Detection of all seven serotypes of foot-and-mouth disease virus by real-time, fluorogenic reverse transcription polymerase chain reaction assay. 105(1), 67-80.

Jin-Ho Shin, Hyun-Joo Sohn, Kang-Seuk Choi, Byung-Jun Kwon, Cheong-Up Choi, Jae-Hoon Kim, Eui-Kyung Hwang, Jong-Hyeon Park, Jong-Yeom Kim, Sang-Ho Choi, Ok-Kyung Kim (2003) Identification and Isolation of Foot-and-Mouth Disease Virus from Primary Suspect Cases in Korea in 2000.. 65(1), 1-7.

Stanley Tam, Alfonso Clavijo, Eric Engelhard, Mark Thurmond (2009) Fluorescence-based multiplex real-time RT-PCR arrays for the detection and serotype determination of foot-and-mouth disease virus. 161(2), 183-191.