Development and Validation of Derivative FTIR Spectroscopy for Estimation of Entecavir Monohydrate in its Pure and Pharmaceutical Dosage Forms
Keywords:
entecavir monohydrate, FTIR, second derivative FTIR, sandell#x2019;s sensitivity, statistical analysis
Abstract
We developed a unique analytical technique for the evaluation of Entecavir monohydrate (ETV) in its pharmaceutical dosage form using derivative spectroscopy assisted FTIR. This approach requires the formation of solid pellets of Entecavir using potassium bromide (KBr) with the aid of geometrical mixing. The spectra were calculated by direct measurement technique using reduced path length in the absorbance mode, and the equipment was configured to secure it at 8cm-1 resolution. We scanned the spectra between the ranges of 4000 to 400 cm-1. FTIR spectra drug exhibited overlapped functional group peaks with baseline correction at 1631 cm-1 corresponding to C=O stretching. From these FTIR spectra, we detected intense, clear, and proportional second derivative peaks between 1639.38 and 1620.09 cm-1. These peaks, in the range of concentration 12.5-200 #x3BC;g/mg, obeyed Beer-Lambert#x2019;s law. Therefore, we elected C=O stretching for the quantitative evaluation of Entecavir employing second-order derivative spectroscopy.
Downloads
- Article PDF
- TEI XML Kaleidoscope (download in zip)* (Beta by AI)
- Lens* NISO JATS XML (Beta by AI)
- HTML Kaleidoscope* (Beta by AI)
- DBK XML Kaleidoscope (download in zip)* (Beta by AI)
- LaTeX pdf Kaleidoscope* (Beta by AI)
- EPUB Kaleidoscope* (Beta by AI)
- MD Kaleidoscope* (Beta by AI)
- FO Kaleidoscope* (Beta by AI)
- BIB Kaleidoscope* (Beta by AI)
- LaTeX Kaleidoscope* (Beta by AI)
How to Cite
Published
2020-03-15
Issue
Section
License
Copyright (c) 2020 Authors and Global Journals Private Limited
This work is licensed under a Creative Commons Attribution 4.0 International License.