We developed a unique analytical technique for the evaluation of Entecavir monohydrate (ETV) in its pharmaceutical dosage form using derivative spectroscopy assisted FTIR. This approach requires the formation of solid pellets of Entecavir using potassium bromide (KBr) with the aid of geometrical mixing. The spectra were calculated by direct measurement technique using reduced path length in the absorbance mode, and the equipment was configured to secure it at 8cm-1 resolution. We scanned the spectra between the ranges of 4000 to 400 cm-1. FTIR spectra drug exhibited overlapped functional group peaks with baseline correction at 1631 cm-1 corresponding to C=O stretching. From these FTIR spectra, we detected intense, clear, and proportional second derivative peaks between 1639.38 and 1620.09 cm-1. These peaks, in the range of concentration 12.5-200 μg/mg, obeyed Beer-Lambert’s law. Therefore, we elected C=O stretching for the quantitative evaluation of Entecavir employing second-order derivative spectroscopy.

How to Cite
A KHANAM, Y PADMAVATHI, RAGHAVENDRA BABU, Ashraf. Development and Validation of Derivative FTIR Spectroscopy for Estimation of Entecavir Monohydrate in its Pure and Pharmaceutical Dosage Forms. Global Journal of Medical Research, [S.l.], july 2020. ISSN 2249-4618. Available at: <https://medicalresearchjournal.org/index.php/GJMR/article/view/2165>. Date accessed: 27 sep. 2020.

Organize Conference

We organize seminars /symposium /conferences/workshop/webinar worldwide on innovative topics and fields of Computer Science and research. We also collaborate, partner or sponsor with a view to provide our professional guidance and expertise to conduct in standard way.

Read More

Research Membership

Global Journals Inc., as described in our Corporate Statement, is an educational, research, research publishing, and professional membership organization. Elevation to the ranks of Fellow of the Association of the Research Society in Computer and Member of the Association of the Research Society in Computer is open.

Read More