Objective: In this study our main goal is to evaluate the Knowledge, Attitude and Practice of taking Covid-19 vaccination among the rural and urban people in Patuakhali District. Method: This cross-sectional study was carried out at Tertiary medical College Hospital, Patuakhali District. Bangladesh. Where data were collected from July 2020 to May 2021. A total of 120 participants where in rural 60 participants and in urban 60 participants were included in the study. Results: During the study, in rural group majority of cases belong to 32-43 years age group, 38.3% where as in urban group 51.7% were belong to 44-54 years age group. In both group majority of the cases were HSC passed, 50% and 55%. In urban group most cases had proper knowledge about covid-19 vaccination.