Raphanus sativus Linn. (Radish) is an annual herb of family Cruciferae or Brassicaceae and grown as an edible root. Objectives : The aim of the study is to test the potentiality of different solvent extracts (Ethanol, Methanol, Ethyl Acetate, Chloroform, Benzene, Aqueous hot and Aqueous cold) against various pathogenic bacterial strains E.coli (ATCC-25922), Klebsiella pneumonia (ATCC-27736), Proteus vulgaris (ATCC-6380), Pseudomonas aeruginosa (ATCC-27853), Staphalococcus aureus (ATCC-25923), Shigella sonnie (ATCC-25931), Salmonella typhi (ATCC-25241) and Salmonella paratyphi (ATCC-9150). Methods : The antibacterial activity was performed in vitro using Agar well diffusion assay and diameter of zone of inhibition was measured. Results : Among all the extracts Ethanolic and Methanolic extracts showed maximum antibacterial activity against all the bacterial strain used with a zone of inhibition ranges from 12-21mm and the least activity was observed in Aqueous cold extract with zone of inhibition ranges from 7-9mm. The test results were compared with standard antibiotics chloramphenicol and Ciprofloxacine. Conclusions : The qualitative analysis of different extracts of Raphanus sativus seed reveals the presence of Alkaloids, Flavonoids, Glycosides, Phenols, Tannins, Saponin, Sterols and Protien which may be responsible for the observed antibacterial activity. The results suggest that ethnolic and methnolic extracts can be used in the treatment of infection caused by these bacterial strains used in this study.