Abstract

Chromatographic System Chromatography was performed with a high performance liquid chromatography. The HPLC system was consisted of Shimadzu SCL-10A system controller, UV visible SPD-10AV detector and LC-10AT pump with FUC-10AL VP flow controller wall. Separation was achieved at ambient temperature with Hypersil C18 BDS 250x4.6 column pore size of 5 micron. Chromatographic data was collected and analyzed using CSW32 software. Chromatographic conditions Quantitative analysis of allopurinol was achieved by using an isocratic mode. UV detector was use for the detection of allopurinol. Hypercil C18 BDS 250*4.6 column was used. Flow rate was maintain at 1ml/min with 20 min run time. Preparation of mobile phase The mobile phase was prepared by dissolving 2.72 g NaCH3COO#xB7;3H2O in 3000mL distilled water and correcting the pH to 4.5 with acetic acid 30%. The mobile phase was filtered and degassed before use. The mobile phase was filtered in vacuum filtration assembly having cellulose filter which have pore size 0. 45um (Sartorius company ). Filterd mobile phase was solicited for the removal of bubbles for 10 minutes. (eyela sonicator )improve the community knowledge, attitude and practice towards malaria prevention and control options.