Participation of T, B and NKT Lymphocytes and CD1 Molecule in the Infection by Entamoeba histolytica in Mice

Marcelo Vidigal Caliari; Fabricio Marcus Silva Oliveira; Bernardo Coelho Horta; Luana Oliveira Prata; Andrezza Fernanda Santiago

Authors

Marcelo Vidigal Caliari
Fabricio Marcus Silva Oliveira
Bernardo Coelho Horta
Luana Oliveira Prata
Andrezza Fernanda Santiago

Keywords:

amebic colitis, Entamoeba histolytica, natural killer T lymphocytes

Abstract

Studies have shown that CD1 double negative mice (CD1d -/-) develop larger liver abscesses due to their inability to present amebic antigens to NK T lymphocytes. Therefore, we conducted flow cytometry studies to determine the frequency of NK T, CD4 + T, CD8 + T and B lymphocytes in mice with amebic colitis. The frequency of NK T, CD8 + T and B lymphocytes was reduced in the MLN of mice in the CTRL-CD1 -/-and Eh-CD1 -/-groups compared to the CTRL-WT and Eh-WT groups. There was also a significant decrease in the frequency of B lymphocytes in the spleens of the animals in the Eh-WT group when compared with the CTRL-CD1 -/-, Eh-CD1 -/- and CTRL-WT groups. The results of the flow cytometry analysis highlight the importance of NK T lymphocytes in the immune response of mice to amebic intestinal infection and the importance of CD1 molecules in the activation of T and B lymphocytes.

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Participation of T, B and NKT Lymphocytes and CD1 Molecule in the Infection by Entamoeba histolytica in Mice. (2015). Global Journal of Medical Research, 15(F4), 7-13. https://medicalresearchjournal.org/index.php/GJMR/article/view/992

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References

(1997) WHO Expert Committee on Drug Dependence: Twenty-eighth report.. 72.

J Stanley, Sl (2003) Amoebiasis. 361(9362), 1025-1034.

M Brigl, L Bry, S Kent (2003) Mechanism of CD1drestricted natural killer T cell activation during microbial infection. 4, 1230-1237.

S Moody, S Becker, Y Nuchamowitz, D Mirelman (1998) Identification of significant variation in the composition of lipophosphoglycan-like molecules of E. histolytica and E. dispar. 45, 9-12.

Fms Oliveira, B Coelho, L Prata (2012) Susceptibility to intestinal infection is related to reduction in natural killer T-lymphocytes in C57BL/6 mice. 4, 22-29.

S Smiley, P Lanthier, K Couper (2005) Exacerbated Susceptibility to Infection-Stimulated Immunopathology in CD1d-Deficient Mice. 174, 7904-7911.

H Macdonald (1995) NK1.1+ T cell receptor-alpha/beta+ cells: new clues to their origin, specificity, and function.. 182(3), 633-638.

A Bendelac, M Rivera, S Park, J Roark (1997) Mouse CD1 specific NK1 T cells: development, specificity, an function. 15, 535-562.

M Kronenberg (2007) Toward an understanding of NKT cell biology: progress and paradoxes. 23, 877-900.

H Lotter, N González-Roldán, B Lindner (2009) Natural Killer T cells activated by a lipopeptidophosphoglycan from Entamoeba histolytica are critically important to control amebic liver abscess. 5, 1-11.

Emmanuel Tupin, Yuki Kinjo, Mitchell Kronenberg (2007) The unique role of natural killer T cells in the response to microorganisms. 5(6), 405-417.

Edward Nieuwenhuis, Tetsuya Matsumoto, Dicky Lindenbergh, Rob Willemsen, Arthur Kaser, Ytje Simons-Oosterhuis, Sylvia Brugman, Keizo Yamaguchi, Hiroki Ishikawa, Yuji Aiba, Yasuhiro Koga, Janneke Samsom, Kenshiro Oshima, Mami Kikuchi, Johanna Escher, Masahira Hattori, Andrew Onderdonk, Richard Blumberg (2009) Cd1d-dependent regulation of bacterial colonization in the intestine of mice. 119(5), 1241-1250.

H Arase, N Arase, Ogasawara (1992) An NK1.1 + CD4 + single-positive thymocyte subpopulation that expresses a highly skewed T cell antigen receptor family. 89, 6506-6510.

A Bendelac, R Schwartz CD4C and CD8C T cells acquire specific lymphokine secretion potentials during thymic maturation. 199, 68-71.

M Gomes, J Pesquero, C Furst (1999) An improved method to distinguish Entamoeba histolytica and Entamoeba dispar. 119, 359-362.

A Martinez, M Gomes, J Viana (1996) Isoenzyme profile as parameter to differentiate pathogenic strains of Entamoeba histolytica in Brazil. 38, 407-412.

N Ueda, H Kuki, D Kamimura (2006) CD1d-restricted NKT cell activation enhanced homeostatic proliferation of CD8+ T cells in a manner dependent on IL-4. 18(9), 1397-1404.

J Ventura‐juárez, L Barba‐gallardo, L Muñoz‐fernández, L Martínez‐medina, F Márquez‐díaz, S Sosa‐díaz, M Gerardo‐rodríguez, R González‐romo, R Campos‐rodríguez (2007) Immunohistochemical characterization of human fulminant amoebic colitis. 29(4), 201-209.

A Asgharpour, C Gilchirist, D Baba (2005) Resistance to Intestinal Entamoeba histolytica Infection is conferred by Innate Immunity and Gr-1 + Cells. 73, 4522-4529.

H Kitamura, A Otha, M Sekimoto, M Sato (2000) αgalactosylceramide induces early B-cell activation through IL-4 production by NKT cells. 199, 37-42.

G Galli, S Nuti, S Tavarini (2003) CD1d-restricted help to B cells by human invariant natural Killer T lymphocytes. 1997, 1051-1057.

J Carrero, C Cervantes‐rebolledo, H Aguilar‐díaz, M Díaz‐gallardo, J Laclette, J Morales‐montor (2007) The role of the secretory immune response in the infection by Entamoeba histolytica. 29(7), 331-338.