Biochemical Changes of Mancozeb-Induced AlteBiochemical Changes of Mancozeb-Induced Alternations in Testes and Thyroid gland of Male Ratsrnations in Testes and Thyroid gland of Male Rats

Abstract

Mancozeb manganese ethylene bis dithiocarbamate polymeric complex with zinc salts is a very important protective non-systemic fungicide classified as an ethylene bis dithio-carbamate fungicide Mancozeb used for controlling fungal diseases for a wide variety of crops because of its broad-spectrum as fungicidal effects and high compatibility with agrochemicals The objective of this study was to investigate the results of the fungicide mancozeb at different doses on some biochemical parameters reproductive performance and histological changes in testes and thyroid gland The low amount equals 1 7 of LD50 manczeb-d1 and the high amount equals 1 3 5 of LD50 mancozeb-d1 Amounts of mancozeb mancozeb-d1 and mancozeb-d2 adjusted according to the rat s body weights The results showed that mancozeb decreased plasma testosterone level sperm count viability motility and significantly P 0 05 increased abnormal sperms altered acrosome and abnormal DNA Treatment of rats with manczeb-d1 and mancozeb-d2 significantly P 0 05 decreased T3 Also T4 significantly P 0 05 fell in the group treated with mancozeb-d2 Treatment of rats with mancozeb-d1 and mancozeb-d2 significantly P 0 05 increased TSH Furthermore the histological study showed that exposure to mancozeb reduced the number of mature spermatozoa necrosis and basal vacuoles observed in some tubules Also mancozeb reduces colloid in most follicles resulting in desquamation of the follicular epithelium into the lumen of the thyroid follicles In conclusion despite mancozeb exhibiting low acute toxicity it has been shown to cause detrimental effects on reproduction thyroid gland and its secretion For this reason it is necessary to be careful when using mancozeb in agricultural areas and should take precautions