Investigation of the Presence of Different Animal Species within Processed Meat and Meat Products using PCR Procedures and Development of Risk Models Based on Consumer Health

Authors

  • Harun Cerit

  • Ayse Z. Aroguz

Keywords:

meat, meat products, adultery, fraud, pathogens, DNA typing, PCR

Abstract

Fraudulent imitation and adultery of meat and meat products are fooling the consumers, jeopardizing their health, economical situation and potentially causing harm to religious beliefs. The aim of this project was to search for the existence of such fraudulent imitations and adulteries within processed meat products across different sale points (them being markets) found within 11 municipalities of the Marmara Regionusing PCR procedures. According to the findings gathered during the study, 25 of the collected samples (4.54%) contained poultry DNA, 5 of them (0.90%) contained house fly DNA, 6 of them (1.09%) contained sheep DNA, 2 of them (0.36%) contained cockroach DNA, 2 of them (0.36%) contained horse DNA and 4 of them (in chicken sausages/ 0.72%) contained bovine DNA as foreign species. Again our findings showed that, for samples not suitable for human consumption in relation to their Escherichia coli parameter of total coliform bacteria quantity, highest value was found within beef salami and chicken sausage.

How to Cite

Investigation of the Presence of Different Animal Species within Processed Meat and Meat Products using PCR Procedures and Development of Risk Models Based on Consumer Health. (2017). Global Journal of Medical Research, 17(G1), 13-23. https://medicalresearchjournal.org/index.php/GJMR/article/view/1386

References

John Dooley, Kelly Paine, Stephen Garrett, Helen Brown (2004) Detection of meat species using TaqMan real-time PCR assays. 68(3), 431-438.

Kontrol Gıda, Müdürlüğü Genel (2014) Et ve Et Ürünleri Uygulama Talimatı.

Robert Pinner, A Schuchat, B Swaminathan, P Hayes, K Deaver, R Weaver, B Plikaytis, M Reeves, C Broome, J Wenger (1992) Role of Foods in Sporadic Listeriosis. 267(15), 2046.

J Farber, P Peterkin (1991) Listeria monocytogenes, a food-borne pathogen. 55(3), 476-511.

S Edberg, E Rice, R Karlin, M Allen (2000) Escherichia coli: the best biological drinking water indicator for public health protection. 88(S1), 106S-116S.

Georgia Sampson (2001) Assessing the Efficacy of Bacteriological Analytical Manual (BAM) Enrichment Broths for Detection of Salmonella spp. in Meat Analogs.

O İlhak, A Arslan (2006) Identification of meat species by polymerase chain reeaction (PCR) technique. 31(3), 159-163.

Z Kesmen, F Sahin, H Yetim (2007) PCR assay for the identification of animal species in cooked sausages. 77, 649-663.

Z Kesmen, F Sahin, H Yetim (2010) PCR assay for the identification of animal species in cooked sausages. 77(4), 649-653.

H Malkawi, R Gharaibeh (2004) Rapid and Simultaneous Identification of two Salmonella enterica Serotypes Enteritidis and Typhimurium from Chicken and Meat Products by Multiplex PCR. 3(1), 44-48.

M Miyazaki, K Ohyama, D Dunlap, F Matsumura (1996) Cloning and sequencing of the para -type sodium channel gene from susceptible and kdr -resistant German cockroaches (Blattella germanica) and house fly (Musca domestica). 252, 61-68.

M Burkhard, B Cornelia, H Reiner (2003) Evaluation of Salmonella spp. specific primer-sets for the validation within the Food PCR project. 1, 12277.

L Hyungkun, H Kyung, H Chong-Hae, B Gyung-Jin, S Weon (2005) Comparison of four molecular typing methods for the differentiation of Salmonella spp. 105, 411-418.

P Kuhnert, S Capaul, J Nicolet, J Frey (1996) Phylogenetic positions of Clostridium chauvoei and Clostridium septicum based on 16S rRNA gene sequences. 46, 1174-1176.

Miruka Onyango, Beniam Ghebremedhin, Eliud Waindi, Rose Kakai, Wolfgang Rabsch, Erhard Tietze, Wolfgang König, Brigette König (2009) Phenotypic and genotypic analysis of clinical isolates Salmonella serovar Typhimurium in western Kenya. 3(9), 685-694.

X Zhou, X Jiao (2005) Molecular grouping and pathogenic analysis of Listeria monocytogenes of clinical and food origin. 16, 867-872.

R Başkaya, T Karaca, O Çakmak, A Yıldız, M Yörük (2006) İstanbul'da Satışa Sunulan Hazır Kıymaların ve Köftelerin Histolojik, Mikrobiyolojik ve Serolojik Kalitesi. 9, 647-648.

Emek Dumen, Funda Sezgin (2009) Pastanelerde İstihdam Edilen Personelin Ellerinin Mikrobiyolojik Yükünün Kişisel ve Yaşam Biçimleri Özelliklerine Göre Modellenmesi. 15(4), 491-498.

Yhp Hsieh, M Johnson, C Wetzstein, N Gren (1996) Detection of species adulteration in pork products using agar -gel immunodiffusion and enzyme linked immunosorbent assay. 19, 1-13.

Ö Türkyılmaz, B Kafa, Y İzan, Ş Sava (2009) Çiğ et ve et ürünlerinde AGID yöntemi ile türlerin tespiti. 31(45), 15-20.

N Türk, B Kafa, Y Izan (2005) Et ve et ürünlerinde tür tayini. 5, 19-21.

U Günşen, A Aydın, B Ovalı, Y Coşkun (2006) Çiğ et ve ısıl işlem görmüş et ürünlerinde ELISA tekniği ile farklı et türlerinin tespiti. 32(2), 45-52.

Abdul-Rauf Um Ammar, M Beuchat, L (1996) Isolation of Escherichia coli O157:H7 from Egyptian foods. 29, 423-426.

M Doyle, J Schoeni (1987) Isolation of Escherichia coli O157:H7 from retail fresh meats and poultry. 53(10), 2394-2396.

M Doyle, D Cliver (1990) Escherichia coli, Chapter 13. 209-215.

Investigation of the Presence of Different Animal Species within Processed Meat and Meat Products using PCR Procedures and Development of Risk Models  Based on Consumer Health

Published

2017-08-31

How to Cite

Investigation of the Presence of Different Animal Species within Processed Meat and Meat Products using PCR Procedures and Development of Risk Models Based on Consumer Health. (2017). Global Journal of Medical Research, 17(G1), 13-23. https://medicalresearchjournal.org/index.php/GJMR/article/view/1386