Oxidative Stress in Dogs in Different Stages of Chronic Kidney Disease

Abstract

The objective of this study to quantify oxidative stress in dogs with naturally acquied chronic kidney disease (CKD), considering the four stages of disease progression, using as markers reactive substances to thiobarbituric acid. Five groups of dogs, all aged between four to 18 years old. Were studied the control group was composed of healthy animals (CG, n = 17), CKD stage 1 group (CKD-1, n = 12), CKD stage 2 group (CKD-2, n = 10), CKD stage 3 group (CKD-3, n = 13) and CKD stage 4 group (CKD-4, n = 10). Oxidative stress was measured by antioxidante power, using as markers reactive substances to thiobarbituric acid. Data (means of duplicates) were submitted to analysis of variance (One-way ANOVA) nonparametric (Kruskal-Wallis) (α =0.05). The results were expressed as mean±standard error of the mean. The serumcreatinine values guided the classification of patients as CG, CKD-1, CKD-2, CKD-3 and CKD-4 andwere 1.02±0.02 mg/dL, 1.06±0.05 mg/dL, 1.80±0.03 mg/dL, 3.39±0.21 mg/dL and 6.00±0.28 mg/dL, respectively. The results related to antioxidant power (GC) 0.032±0.002 mmol/μL, (CKD-1) 0.030±0.002 nmol/μL, (CKD-2) 0.028±0.003 nmol/μL, (CKD-3) 0.027±0.003 nmol/μL and (CKD-4) 0.025 ± 0.002 nmol/μL.