Trichrome Stain for Diagnosis of Amoebae in Parasitology Laboratory

Abstract

For many years the trichrome staining technique (Tricr#xF3;micWheatley) has been considered as the most important technique for the identification ofthe most common intestinal protozoa and popularin parasitology (1). Currently the mostsensiblidad procedure for detecting and identifying protozoa trophozoites stool sample as it helps to easily highlight the morphology of amoebic cysts and trop-hozoites however, the procedure is complicated and tediousto perform and require at seven different reagents which is probably the most critical especially in laboratories with limited staff, this makes it complicated the routine use of this technique in most of the clinical laboratory, using koplic additionally facilitates reagent contamination by repeated use.(4,5)