Compositional Analysis, Antioxidant and Antimicrobial Potential of the Seed of Annona cinerea Dunal Grown in Nigeria

Abstract

Seed of Annona cinerea grown in Nigeria was investigated for its secondary metabolites and antioxidant potential using Gas Chromatography-Mass Spectrometry GC-MS 2 2 - dyphenyl-1-picrylhydrazyl DPPH and 2 6-ditert-butyl-4- 3 5-ditert-butyl-4- 1- oxidanylphenyl methylidene cyclohexa-2 5-dien-1-one Galvinoxyl respectively The antibacterial activity of the seed extract was evaluated on eleven 11 pathogenic bacteria using agar well diffusion method at different concentrations of the extract Twenty-seven 27 therapeutically active secondary metabolites were identified in the seed extract using GC-MS and the principal constituents identified were 3-O-methyl-d-glucose 52 14 -sitosterol 11 79 desulphosinigrin 6 16 and -tocopherol 5 84 The extract also displayed high DPPH and galvinoxyl radical scavenging activity with IC50 values of 5 0 and 100 gml-1 The zones of inhibition ranged from 10-30 mm against all tested bacteria The antibacterial index AI ranged between 0 5-25 This study demonstrated that the seed of A cinerea could be a potential source of natural antioxidants and antimicrobial agents