Antibacterial Efficacy of Pap Slurry Liquor on Some Diarrhogenic Organisms

Abstract

The antibacterial potential of pap slurry liquor on four diarrheal associated organisms (Escherichia coli, Staphylococcus aureus, Salmonella tyhpi, and Shigella dysenteriae) was screened for. The pap slurry liquor was obtained in the laboratory from a pap (made from yellow Zeamays grains) which was allowed to ferment at ambient temperature of 28±1 o C for 72 hours at relative humidity 75±5% by the maize natural microflora. The in vitro screening of different concentrations (100, 90:10, 80:20, 70:30 and 60:40 v/v) of the pap slurry liquor on the test isolates that was carried out using disc diffusion method, revealed a linear relationship antibacterial activities on all the test isolates. The minimum inhibitory concentration was observed at 90:10 v/v for E. coli and S. aureus and 80:20 v/v for S. typhi and S. dysenteriae while the minimum bactericidal concentration was observed at100v/v for E. coli and S. aureus and 90:10 v/v for S. typhi and S. dysenteriae. All the data obtained were subjected to one way analysis of variance at 0.05 significant levels using the New Duncan's Multiple Range Test. The results from this study showed the antibacterial efficacy of pap slurry on the test isolates and therefore could be used in the treatment of diarrhea caused by these selected pathogens.